Kidney function status of Wistar rat treated with ethanolic extract of Phyllanthus amarus leaves owing to diethylnitrosamine intoxication

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Vol. 19 No. 1 (2022)
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Articles

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OJOCHENEMI EJEH YAKUBU ♥
Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari. PMB 1020 Katsina-Ala Road, Wukari, Taraba State, Nigeria
DAVID CHRISTOPHER BANDO
1. Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari. PMB 1020 Katsina-Ala Road, Wukari, Taraba State, Nigeria; 2. Bioresources Development Centre, National Biotechnology Development Agency. Jalingo, Taraba State, Nigeria
MOSES ZEPHANIAH
Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari. PMB 1020 Katsina-Ala Road, Wukari, Taraba State, Nigeria
ISAAC JOHN UMARU
Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari. PMB 1020 Katsina-Ala Road, Wukari, Taraba State, Nigeria
MICHAEL SUNDAY ABU
Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari. PMB 1020 Katsina-Ala Road, Wukari, Taraba State, Nigeria

Abstract

Abstract. Yakubu OE, Bando DC, Zephaniah M, Umaru IJ, Abu MS. 2021. Kidney function status of Wistar rat treated with ethanolic extract of Phyllanthus amarus leaves owing to diethylnitrosamine intoxication. Asian J Trop Biotechnol 19: 6-9. The kidney plays a central role in detoxification and excretion of harmful metabolites and therefore is susceptible to toxicity by xenobiotics. This research investigated the possible modulatory effect of Phyllanthus amarus Schumach & Thonn on diethylnitrosamine (DEN) induced nephrotoxicity in rats. Wistar rats received a single dose intraperitoneal injection of DEN. Twenty (20) rats were randomly allocated into four groups of five (5) rats each, and toxicity was induced using a single dose of DEN at 200 mg/kg intraperitoneally. Treatment was carried out using P. amarus extract at 200 mg/kg for 14 days, while silymarin was used as a standard drug orally at 5mg/kg. Kidney function parameters (Urea, Creatinine, sodium (Na+)), Potassium (K+), and Chloride (Cl-) were determined. After 2 weeks, DEN-induced rats showed renal injury evidenced by the significant increase in circulating kidney function markers. Results showed that concurrent supplementation of P. amarus significantly (p?0.05) modulated kidney function markers and prevented renal tissue damage induced by DEN. In conclusion, P. amarus prevents DEN-induced nephrotoxicity via attenuation of oxidative stress and alleviation of the antioxidant defense system.

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