Hydrolases secreting, heavy metal-resistant halophilic bacteria isolated from metal dumpsites

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Vol. 19 No. 1 (2022)
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CHIOMA FLORENCE MGBODILE
Department of Microbiology, Faculty of Biological Sciences, University of Nigeria. Nsukka, Enugu State, Nigeria
UZOAMAKA OTUTU
Department of Microbiology, Faculty of Biological Sciences, University of Nigeria. Nsukka, Enugu State, Nigeria
STEPHANIE ONUOHA
Department of Microbiology, Faculty of Biological Sciences, University of Nigeria. Nsukka, Enugu State, Nigeria
UCHENNA EZE
Department of Microbiology, Faculty of Biological Sciences, University of Nigeria. Nsukka, Enugu State, Nigeria
TOBECHUKWU UGWUOJI
Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University. PMB 5025, Awka, Anambra State, Nigeria
OBIANUJU NNABUIFE
Department of Microbiology, Faculty of Biological Sciences, University of Nigeria. Nsukka, Enugu State, Nigeria
TOCHUKWU NWAMAKA T. NWAGU ♥
Department of Microbiology, Faculty of Biological Sciences, University of Nigeria. Nsukka, Enugu State, Nigeria

Abstract

Abstract. Mgbodile CF, Otutu U, Onuoha S, Eze U, Ugwuoji T, Nnabuife O, Nwagu TNT. 2022. Hydrolases secreting, heavy metal-resistant halophilic bacteria isolated from metal dumpsites. Asian J Trop Biotechnol 19: 11-19. Microorganisms with the potential to accumulate heavy metals are currently under investigation for application in environmental detoxification. The current study aimed to isolate heavy metal tolerant bacteria from metal dump sites. The soil samples were sourced from two metal dumpsites in Eastern Nigeria. The pH and metal content of the samples were evaluated. Microorganisms were screened for tolerance of lead, copper, silver and chromium. Isolated strains were characterized and identified by molecular techniques. All isolates grew in 1000 ppm Pb, Cu and Cr. The optimal temperature for their growth was 37oC. While isolates B and C grew optimally at 12.5% NaCl, the growth rate increased for isolate A (i.e., 7.5%>12.5%>15%). The three isolates produced extracellular protease, and inulinase isolates B and C produced amylase, while isolating A produced xylanase. Isolate A, B, and C were identified as Pseudomonas asiatica, Sphingobacterium caeni, and Burkholderia cenocepacia. The bacteria were resistant to a wide range of antibiotics, including ampicillin (30 mcg/disc), ceporex (10 mcg/disc), and streptomycin (30 mcg/disc). These results indicate that the bacterial strains have the potential as sources of inoculants for the bioremediation of heavy metal contaminated environments and application in various industrial processes where metal-resistant organisms and their hydrolases are required.

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