Effects of aqueous extract of polyherbal formulation on acetaminophen-induced hepatotoxicity in albino rats

Abstract

Abstract. Abu MS, Mashi RL, Lawal JY, Nathan F. 2023. Effects of aqueous extract of polyherbal formulation on acetaminophen-induced hepatotoxicity in albino rats. Asian J Trop Biotechnol 20: 37-41. The present study investigated the hepatoprotective activity of aqueous extract of the polyherbal mixture (Carica papaya L., Allium sativum L., Curcuma longa L., and Azadirachta indica A.Juss.) against acetaminophen-induced liver damage in rats. The Wistar albino rats of either sex were divided into every five animals into six groups. They were given the following seven-day treatment, i.e., paracetamol 500 g/kg BW p.o. to induce hepatotoxicity; Silymarin (140 mg/kg BW p.o.) as a reference standard; and three doses of polyherbal extract (100 mg/kg BW p.o., 300 mg/kg BW p.o., and 500 mg/kg BW p.o.). Blood collection by cardiac puncture was carried out after 24 h of the last administration and analyzed for various serum parameters (Serum Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP), Total Bilirubin (TB), Albumin (ALB), Total Protein (TP), Triglycerides (TGL) and Total Cholesterol (TCHOL). Treatment of the aqueous extract of the polyherbal mixture reduced the elevated levels of AST, ALT, ALP, TB, TGL, and TCHOL. It increased ALB and TP levels to indicate the repair of hepatic damage and demonstrated the aqueous extract hepatoprotective activity of the polyherbal mixture. The aqueous extract of the polyherbal mixture at doses of 100 mg/kg BW, 300 mg/kg BW and 500 mg/kg BW have significant (p<.005) effects on the liver of the paracetamol-induced hepatotoxicity rat model.