Immunostimulator effect of propolis on T lymphocyte proliferation and the mammary tumor cells’ viability in mice in vitro

Abstract

The development of new medicines as immunostimulator agent has being increased according to the development of lethal diseases, for example tumor that suppress the immune responses. Propolis, which is resinous substance produced by honeybees known to have antimicrobial, antiinflammatory, antioxidant, immunostimulator, and anti-tumor activities. The aims of this experiment were to study the effect of propolis as immunostimulator agent in proliferation of mice-suffered with mammary tumor T lymphocyte and the mammary-tumor cells’ viability after been treated with stimulated T lymphocyte. Three different dozes of propolis used in this experiment were 0.5 μg/mL, 1.5 μg/mL, 4.5 μg/mL, and added in cell culture to measure the proliferation of T lymphocyte. Proliferation activity of T lymphocyte was examined with 3-(4.5-dimethyl-2-thiazolyl)- 2.5-diphenyl-2H-tetrazolium bromide (MTT) assay. Data was analyzed by using Analysis of Variance (ANOVA) then followed by Duncan Multiple Ranges Test (DMRT) 5%. The result showed that the proliferation of T lymphocyte was increased after addition of propolis. The highest OD of T lymphocyte was reached in addition of propolis 4.5 μg/mL (2.363). In the examination of mice-mammary tumor cells’ viability, stimulated T lymphocyte was added to tumor cells culture and the results showed that the viability of tumor cells was decreased according to the increasing of T lymphocyte proliferation. The lowest tumor cells’ viability was reached in addition of stimulated T lymphocyte with propolis 4.5 μg/mL (4.667).