Keragaman genetik simbion alga Zooxanthellae pada anemone laut Stichodactyla gigantea (Forsskal 1775) hasil reproduksi aseksual

Abstract

Rifa’i MA. 2012. Genetic diversity of alga Zooxanthellae symbiont in the giant carpet anemone (Stichodactyla gigantea, Forsskål 1775) from asexual reproduction. Bioteknologi 9: 49-56. The aim of the study was to discover the genetic diversity of the algae symbiont of zooxantellae that have the symbiosis with the giant carpet anemone (Stichodactyla gigantea, Forskal 1775) which is resulted from asexual reproduction with the technique of fragmentation. The study was conducted for ten months, from October 2011 to July 2012 in The Hatchery of University of Hasanuddin’s Marine Station in Barrang Lompo Island and coral reefs area of Barrang Lompo Island. The series of study was started with parental collection and acclimatization of anemones, fragmentation of the body, culturing anemones in the coral reefs area and collection of algae zooxanthellae for PCR-ISSR analysis. Genetic diversity was analyzed using cluster analysis and making a family tree dendrogram using UPGMA method through NTSYS program. The result showed that all primer produce polymorphic bands with a range of 16.67%- 66.67%. The results of the genetic variation of zooxanthellae indicate that polymorphism by 37.93%. Whereas, based on the analysis of genetic distance indicate was not similar by 19% from two groups of the primary anemones, that were result of four-fragmentation anemones (AF4) compared to natural anemones (AA) and two-fragmentation anemones (AF2).