The effect of anting-anting (Acalypha indica) extract with malondialdehyde (MDA) levels of Balb/C mice induced streptozotocin.

Abstract

Perlitasari Y, Prasetyo DH, Martini. 2016. The effect of anting-anting (Acalypha indica) extract with malondialdehyde (MDA) levels of Balb/C mice induced streptozotocin. Bioteknologi 13: 83-90. The objective of the research was to examine the effect of anting-anting (Acalypha indica Linn) extract with malondialdehyde levels of Balb/C mice induced streptozotocin. This study was a laboratory experimental post test only control group design. Diabetes was induced by intraperitoneal streptozotocin doses of 65 mg/kg body weight dissolved in 0,02 M citrate saline buffer. The subjects used were 32 male mice divided into 4 groups: (i) Non-diabetic control group; (ii) Diabetic group; (iii) Treated-diabetic group with metformin doses of 1,3 mg/mice/day; and (iv) Treated-diabetic group with anting-anting extract dose 1000 mg/kg body weight/day. After 2 weeks, malondialdehyde level was measured. Blood was collected in clean centrifuge tube with EDTA from orbitalis sinus and centrifuged (15 minutes at 3000 rpm, 37°C). The serum used for MDA assay in Biochemistry Laboratory, Faculty of Medicine Gadjah Mada University. The data obtained were statistic analyzed by Anova using SPSS Programme for Microsoft Windows release 16.0. Significance was set at p<0,05. MDA levels of non-diabetic control group 0,211±0,145 μmol/L, diabetic group 0,363±0,208 μmol/L, treated-diabetic group with metformin 0,389 ± 0,187 μmol/L and treated-diabetic group with anting-anting extract 0,309±0,145 μmol/L. Statistical analyses with Anova showed that the result was not significance (p=0,199). The experiment result showed that anting-anting (Acalypha indica Linn) extract doses 1000 mg/kg body weight/day can reduce the MDA levels in Balb/C mice induced streptozotocin but statistically insignificance (p=0,538).