Effect of chromated copper arsenate on protein, carbohydrate, and chlorophyll content of tropical Eucalyptus and Acacia species

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Vol. 20 No. 2 (2023)
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Articles

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NARAYANAPPA NAGARAJA ♥
UGC-Human Resource Development Centre, Bangalore University. Jnana Bharathi Campus, Bengaluru, Karnataka 560056, India
BETTAIAH MALLAMMA RATHNA KUMARI
Department of Botany, Government First Grade College. Vijayanagar, Bengaluru, Karnataka 560040, India

Abstract

Abstract. Kumari BMR, Nagaraja N. 2023. Effect of chromated copper arsenate on protein, carbohydrate, and chlorophyll content of tropical Eucalyptus and Acacia species. Asian J Trop Biotechnol 20: 56-61. Contamination of heavy metals could cause morphological, physiological, and biochemical changes in plants. Chromated Copper Arsenate (CCA) is a wood preservative that contains Cr, Cu, and As. Leaching these heavy metals into agricultural fields from moderate to higher levels causes a serious threat to the ecosystem. A pot experiment was conducted to evaluate the effect of various concentrations of CCA (250-2,500 mg kg-1 soil) on the protein, carbohydrate, and chlorophyll content of Eucalyptus and Acacia seedlings using commercially available C-type CCA with a proportion of Cr (CrO3-47.5%), Cu (CuO-18.5%), and As (As2O5- 35%). The quantitative estimation of total carbohydrate, protein, and chlorophyll content in control and treated seedlings was carried out by spectrophotometric methods. Results showed that the high concentrations of CCA significantly (p ?0.05) reduced protein, carbohydrate, and chlorophyll content of Eucalyptus and Acacia seedlings. The lowest amount of total carbohydrates, proteins, and chlorophyll content found in E. citriodora Hook. and E. tereticornis Sm. were 0.97±0.05 and 1.70±0.17 mg g-1, 0.42±0.08 and 0.45 ±0.02 mg g-1 and 0.11±0.04 and 0.07±0.03 mg g-1 at 2,500 mg kg-1 soil CCA respectively. Furthermore, the lowest total carbohydrates, proteins, and chlorophyll content in A. mangium seedlings were 1.91±0.43, 0.52±0.13, and 0.13±0.01 mg g-1 at 2,500 mg kg-1 soil CCA, respectively. The present study advocates that the higher concentrations of CCA affect the biochemical parameters of Eucalyptus and Acacia tree species.

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