Phylogenetic relationship and biotechnological use potential of epiphytic Actinomycetota species isolated from seagrasses from the coast of Tanzania
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https://orcid.org/0000-0003-2584-7606
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Abstract. Mbusi LD, Lyantagaye SL, Lyimo TJ. 2024. Phylogenetic relationship and biotechnological use potential of epiphytic Actinomycetota species isolated from seagrasses from the coast of Tanzania. Asian J Trop Biotechnol 21: 41-51. Actinomycetota, previously recognized as actinobacteria, has demonstrated considerable promise as a valuable reservoir of secondary metabolites with potential pharmaceutical applications. This study examined the diversity, antimicrobial properties, and cytotoxic effects of epiphytic Actinomycetota species isolated from the seagrasses Thalassia hemprichii and Syringodium isoetifolium. Therefore, 12 strains of Actinomycetota were isolated through a process involving assessment of their morphological and biochemical characteristics, along with phylogenetic analysis using partial sequences of the 16S rRNA gene. The identified strains were associated with eight phylogenetic genera: Cellulosimicrobium, Corynebacterium, Microbacterium, Rhodococcus, Arthrobacter, Leucobacter, Dietzia, and Micrococcus. The findings unveiled five potential new species within Actinomycetota. Seven of the 12 strains displayed antimicrobial effects against at least one human pathogen tested. Notably, the Microbacterium strain SIP6 exhibited extensive antimicrobial efficacy against all the pathogens under examination. Toxicity tests revealed that only two strains (Microbacterium (THP6) and Micrococcus (SIP14)) were nontoxic, with the lowest LC50 values of 3836.7 and 1243.4 ?g/mL, respectively, while the remaining extracts were toxic. This study marks the initial discovery of epiphytic Actinomycetota strains from seagrasses in the western Indian Ocean. The isolated epiphytic Actinomycetota strains, some of which are novel, showed potential for bioactive metabolites that hold promise for biotechnological use.
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