Cattle urine as a low-cost medium for accelerate growth, biomass productivity, and lipid production of Botryococcus braunii: Future energy

Abstract

Abstract. Hidayati Z, Arofah AN, Fakhira J. 2018. Cattle urine as a low-cost medium for accelerate growth, biomass productivity, and lipid production of Botryococcus braunii: future energy. Bioteknologi 15: 51-54. The increasing necessities of global energy can trigger an energy crisis. Microalgae is a unicellular microorganism which has potency as bioenergy, such as biofuel, and efficient alternative to substitute fossil resources. Botryococcus braunii is a microalgae that has a high lipid concentration of around 25-80%, and it is this characteristic which shows its potency to produce biofuel. The research purpose was to utilize cattle urine as an alternative medium for growth of B. braunii. Some of the components in cattle urine are 95% water, urea, 2.5% mineral salt, hormone, enzyme, 69% nitrogen, sulfur, and magnesium. The components of cattle urine can substitute macronutrient and micronutrient in others synthesis medium. This research was designed by using Random Design Complete (RAL) with seven variables: control culture B. braunii (medium macronutrient+ micronutrient Johnson); cultures B. braunii with 5%, 7.5%, 10%, 12.5%, 15%, and 17.5% concentration of cattle urine+micronutrient Johnson. The methods of this research were divided into three steps: preparation, testing, and analysis of biomass also lipid extraction. The data from the observations of biomass were analyzed with linear regression equation. All of data was analyzed in Microsoft Excel. The results showed in culture B. braunii with a 12.5% of concentration cattle urine, that it can accelerate the growth of cell B. braunii being maximal which production 1.425 mg/L biomass in days 12. Additionally, B. braunii culture with 12.5% of concentration cattle urine can accelerate biomass productivity of B. brauni which production 9.018 g/L biomass. Whereas with just added cattle urine as medium is not given significantly to lipid production.