Screening of quorum quenching activity of rhizobacteria against Pectobacterium carotovorum subsp. carotovorum
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Lestari SR, Choliq FA, Sektiono AW, Hadi MS, Aditya HF, Rahmadhini N, Kusuma RM, Setiawam Y. 2022. Screening of quorum quenching activity of rhizobacteria against Pectobacterium carotovorum subsp. carotovorum. Biodiversitas 23: 4336-4342. Quorum sensing (QS) is a mechanism for determining the virulence of the phytopathogenic bacterium Pectobacterium carotovorum. P. carotovorum carries out the QS mechanism with the chemical compound acyl-homoserine lactone (AHL) to signal and carry out its pathogenicity and virulence. The pathogenicity of P. carotovorum can be prevented through quorum quencher (QQ) activity derived from bacteria that degrade AHL compounds by producing AHL-lactonase. In the present investigation, AHL-lactonase-producing bacteria were isolated from the rhizosphere of carrot cultivation in Bumiaji Sub-District, City of Batu, East Java, Indonesia. The isolated bacteria were screened for quorum quenching activity. Out of thirty-seven isolates, 7 rhizospheric bacteria showed QQ activity against the Chromobacterium violaceum bioindicator. The hypersensitivity test on tobacco plants showed that, five strains SRL07, SRL09, SRL15, SRL21, and SRL35 did not cause a hypersensitivity response. The five isolates were able to suppress the virulence of P. carotovorum in carrot tubers. The 16S rRNA gene sequences of the five isolates had the highest similarity to Bacillus thuringiensis, B. subtilis, and B. cereus. The five isolates were detected to have the gene encoding AHL-lactonase (aiiA). The present study provides new information about the QQ activity of rhizosphere and its potential as a quorum quencher against P. carotovorum.
##plugins.themes.bootstrap3.article.details##
References
Abd-El-Khair H, Abdel-Gaied TG, Mikhail MS, Abdel-Alim AI, El-Nasr HIS. 2021. Biological control of Pectobacterium carotovorum subsp. carotovorum, the causal agent of bacterial soft rot in vegetables, in vitro and in vivo tests. Bull Natl Res Cent 45(1): 37. DOI: 10.1186/s42269-021-00491-4.
Baltenneck J, Reverchon A, Hommais F. 2021. Quorum Sensing Regulation in Phytopathogenic Bacteria. Microorganisms 9(2): 239. DOI: 10.3390/microorganisms9020239.
Cao Y, He A, Zhou Z, Zhang M, Mao W. 2012. Orally administered thermostable N-acyl homoserine lactonase from Bacillus sp. strain AI96 attenuates Aeromonas hydrophila infection in zebrafish. Appl Environ Microbiol 78(6): 1899–1908. DOI: 10.1128/AEM.06139-11.
Chan KG, Atkinson S, Mathee K, Sam CK, Chhabra SR. 2011. Characterization of N-acylhomoserine lactone-degrading bacteria associated with the Zingiber officinale (ginger) rhizosphere: Co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia. BMC Microbiol 11(1): 51. DOI: 10.1186/1471-2180-11-51.
Chen F, Gao Y, Chen X, Yu Z, Li X. 2013. Quorum Quenching Enzymes and Their Application in Degrading Signal Molecules to Block Quorum Sensing-Dependent Infection. Int J Mol Sci 14(9): 17477. DOI: 10.3390/IJMS140917477.
Chen R, Zhou Z, Cao Y, Bai Y, Yao B. 2010. High yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromonas hydrophila mortality in aquaculture. Microb Cell Fact 9: 39. DOI: 10.1186/1475-2859-9-39.
Dong W, Zhu J, Guo X, Kong D, Zhang Q. 2018. Characterization of AiiK, an AHL lactonase, from Kurthia huakui LAM0618T and its application in quorum quenching on Pseudomonas aeruginosa PAO1. Sci Rep 8(1): 6013. DOI: 10.1038/s41598-018-24507-8.
Eka P, Rusmana I, Akhdiya A. 2016. AHL-lactonase characteristics of Bacillus thuringiensis SGT3g and its effectiveness in inhibiting pathogenicity of Dickeya dadantii. Malaysian Jounal Microbiol. 12: 315–321. DOI: 10.21161/mjm.80515.
Fan B, Blom J, Klenk HP, Borriss R. 2017. Bacillus amyloliquefaciens, Bacillus velezensis, and Bacillus siamensis Form an “Operational Group B. amyloliquefaciens” within the B. subtilis Species Complex. Front Microbiol 8. DOI: https://www.frontiersin.org/article/10.3389/fmicb.2017.00022.
Fan X, Ye T, Li Q, Bhatt P, Zhang L. 2020. Potential of a Quorum Quenching Bacteria Isolate Ochrobactrum intermedium D-2 Against Soft Rot Pathogen Pectobacterium carotovorum subsp. carotovorum. Front Microbiol 11: 898. DOI: 10.3389/fmicb.2020.00898.
Fitriyah D, Wahyudi A, Rusmana I. 2015. Characterization of Bacteria Producing Acyl Homoserine Lactone (AHL) Lactonase from Agricultural Lands. Adv Environ Biol. 9: 140–148.
Garge SS, Nerurkar AS. 2016. Attenuation of Quorum Sensing Regulated Virulence of Pectobacterium carotovorum subsp. carotovorum through an AHL Lactonase Produced by Lysinibacillus sp. Gs50. PLoS One 11(12): e0167344–e0167344. DOI: 10.1371/journal.pone.0167344.
Ghosh R, Mandal NC. 2022. Chapter 19 - Quorum sensing in rhizosphere engineering. In: Dubey RC, Kumar PBTRE, editors. Academic Press. p. 355–381
Huang J, Shi Y, Zeng G, Gu Y, Chen G. 2016. Acyl-homoserine lactone-based quorum sensing and quorum quenching hold promise to determine the performance of biological wastewater treatments: An overview. Chemosphere 157: 137–151. DOI: https://doi.org/10.1016/j.chemosphere.2016.05.032.
Jayanna S, Umesha S. 2017. Quorum quenching activity of rhizosphere bacteria against Ralstonia solanacearum. Rhizosphere 4. DOI: 10.1016/j.rhisph.2017.05.007.
Khoiri S, Damayanti TA. 2017. Identification of Quorum Quenching Bacteria and Its Biocontrol Potential Against Soft Rot Disease Bacteria, Dickeya dadantii. Agrivita 39(81): 45–55.
Kothari V, Sharma S, Padia D. 2017. Recent research advances on Chromobacterium violaceum. Asian Pac J Trop Med 10(8): 744–752. DOI: https://doi.org/10.1016/j.apjtm.2017.07.022.
LaSarre B, Federle MJ. 2013. Exploiting quorum sensing to confuse bacterial pathogens. Microbiol Mol Biol Rev 77(1): 73–111. DOI: 10.1128/MMBR.00046-12.
Lee S, Park SK, Kwon H, Lee SH, Lee K. 2016. Crossing the Border between Laboratory and Field: Bacterial Quorum Quenching for Anti-Biofouling Strategy in an MBR. Environ Sci Technol 50(4): 1788–1795. DOI: 10.1021/acs.est.5b04795.
Máisuría V, Nerurkar A. 2013. Characterization and differentiation of soft rot causing Pectobacterium carotovorum of Indian origin. Eur J Plant Pathol 136: 87–102. DOI: 10.1007/s10658-012-0140-0.
Marquez-villavicencio MP, Weber B, Witherell RA, Willis DK, Amy O. 2011. The 3-Hydroxy-2-Butanone Pathway Is Required for Pectobacterium carotovorum Pathogenesis. PLoS One 6(8). DOI: 10.1371/journal.pone.0022974.
McClean KH, Winson MK, Fish L, Taylor A, Chhabra SR. 1997. Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. Microbiology 143 ( Pt 12): 3703–3711. DOI: 10.1099/00221287-143-12-3703.
Natrah FMI, Defoirdt T, Sorgeloos P, Bossier P. 2011. Disruption of bacterial cell-to-cell communication by marine organisms and its relevance to aquaculture. Mar Biotechnol (NY) 13(2): 109–126. DOI: 10.1007/s10126-010-9346-3.
Prazdnova EV, Gorovtsov AV, Vasilchenko NG, Kulikov MP, Statsenko VN. 2022. Quorum-Sensing Inhibition by Gram-Positive Bacteria. Microorg 10(2). DOI: 10.3390/microorganisms10020350.
Rehman ZU, Leiknes T. 2018. Quorum-Quenching Bacteria Isolated From Red Sea Sediments Reduce Biofilm Formation by Pseudomonas aeruginosa . Front Microbiol 9. DOI: https://www.frontiersin.org/article/10.3389/fmicb.2018.01354.
Romero M, Diggle SP, Heeb S, Cámara M, Otero A. 2008. Quorum quenching activity in Anabaena sp. PCC 7120: identification of AiiC, a novel AHL-acylase. FEMS Microbiol Lett. 280(1): 73–80. DOI: 10.1111/j.1574-6968.2007.01046.x.
Rutherford ST, Bassler BL. 2012. Bacterial quorum sensing: its role in virulence and possibilities for its control. Cold Spring Harb Perspect Med 2(11): a012427. DOI: 10.1101/cshperspect.a012427.
Sakr MM, Aboshanab KMA, Aboulwafa MM, Hassouna NAH. 2013. Characterization and complete sequence of lactonase enzyme from Bacillus weihenstephanensis isolate P65 with potential activity against acyl homoserine lactone signal molecules. Biomed Res Int 2013: 192589. DOI: 10.1155/2013/192589.
Tang K, Su Y, Brackman G, Cui F, Zhang Y. 2015. MomL, a novel marine-derived N-acyl homoserine lactonase from Muricauda olearia. Appl Environ Microbiol 81(2): 774–782. DOI: 10.1128/AEM.02805-14.
Utari PD, Vogel J, Quax WJ. 2017. Deciphering Physiological Functions of AHL Quorum Quenching Acylases. Front Microbiol 8: 1123. DOI: 10.3389/fmicb.2017.01123.
Zhang W, Luo Q, Zhang Y, Fan X, Ye T. 2020. Quorum Quenching in a Novel Acinetobacter sp. XN-10 Bacterial Strain against Pectobacterium carotovorum subsp. carotovorum. Microorganisms 8(8): 1100. DOI: 10.3390/microorganisms8081100.
Zhao X, Yu Z, Ding T. 2020. Quorum-Sensing Regulation of Antimicrobial Resistance in Bacteria. Microorg 8(3). DOI: 10.3390/microorganisms8030425.
Most read articles by the same author(s)
- BAMBANG TRI RAHARDJO, FAIZ NASHIRUDDIN MUHAMMAD, YOGO SETIAWAN, AKSHA FEBRYADI, MUHAMMAD IHSAN, DENNIS WIBOWO, ITO FERNANDO , Ant preference for different types of bait at sugarcane plantations in East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 4 (2023)
- HAGUS TARNO, EVY OCTAVIA, TOTO HIMAWAN, YOGO SETIAWAN, Detection of fruit flies (Diptera: Tephritidae) using cue-lure and methyl eugenol in Depok City and Bogor District, West Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 8 (2022)
- WIWIN WINDRIYANTI, NONI RAHMADHINI, ITO FERNANDO, RAMADHANI MAHENDRA KUSUMA, Arthropods discovered on refugio flowering plants in Mangifera indica plantation , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 9 (2023)