Genetic diversity based on SSR markers of 30 Aeridinae sub-tribe orchid genetic resources of Indonesian Ornamental Crop Research Institute, Cianjur, Indonesia
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
MEGA WEGADARA
https://orcid.org/0000-0002-8931-4262
MINANGSARI DEWANTI
https://orcid.org/0000-0003-1635-1529
ERNIAWATI DININGSIH
https://orcid.org/0000-0002-9678-8415
FITRI RACHMAWATI
https://orcid.org/0000-0003-4532-903X
DEWI SUKMA
https://orcid.org/0000-0003-3026-8182
SUDARSONO
♥
https://orcid.org/0000-0003-4688-5628
Abstract
Abstract. Wegadara M, Dewanti M, Diningsih E, Rachmawati F, Sukma D, Sudarsono. 2022. Genetic diversity based on SSR markers of 30 Aeridinaesub-tribe orchid genetic resources of Indonesian Ornamental Crop Research Institute, Cianjur, Indonesia. Biodiversitas 23: 2943-2956. Advances in molecular biotechnology encourage molecular markers to identify plant diversity to become increasingly common. Using SSR markers, this study will determine the genetic diversity and phylogenetic relationships of 30 accessions from five parental genera of Aeridinaeorchids from the IOCRI genetic resource collection. Molecular characterization of 30 orchid sub-tribes Aeridinaegenetic resources of IOCRI was carried out using 17 polymorphic SSR markers. The resulting binary data was then analyzed using the GenAlEX program to determine Na, Ne, Ho, He, and the PowerMarker V3.25 program to determine the values of MAF, D, and PIC. Molecular characterization using 17 SSR primers generated 240 amplified DNA fragments across 30 accessions, with the fragment sizes ranging from 56-4818 bp. Factorial analysis (PCoA) and phylogenetic tree construction clustered the 30 Aeridinae accessions into three major clusters. Accessions belonging to the Vanda genus are clustered into three clusters (Clusters I, II, and III). The Vascostylisis in Cluster I, along with 12 Vanda accessions. Two accessions of Vanda, two Aranthera, three Arachnis, and one Aerides genera are clustered into Cluster III. This study showed that interspecific hybrids among accessions of Vanda within Cluster I and Cluster II should be possible since they are genetically closely related. Intergeneric hybrids among the accession of Vascostylis and Vanda's genera belonging to Cluster I, should also be possible. Furthermore, intergeneric hybrids among Aerides, Arachnis, Aranthera, and Vanda accessions within Cluster III should also be possible. The generated data from this study should be helpful for future Aeridinae breeding activities intended to generate new hybrid varieties.
##plugins.themes.bootstrap3.article.details##
References
Antonetti M, Nin S, Burchi G, Biricolti S. 2021. Himantoglossum adriaticum H. Baumann Himantoglossum robertianum (Loisel.) P. Delforge: A New Interspeci?c Hybrid Assessed by Barcoding Analysis. Plants 10(107): 1-19. doi.org/10.3390/ plants10010107.
Aloysius S, Mercuriani IS, Ratnawati, Sudarsono. 2020. Molecular Characterization of Orchid Variants Spathoglottis plicata Blume Based on RAPD Analysis. Proceedings of the 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020). 528:56-61.
Aung YL, Jin X. 2018. Gastrodia kachinensis (Orchidaceae) – a new species from Myanmar. PhytoKeys (94):23-29.
Bhandari HR, Bhanu AN, Srivastava K, Singh MN, Shreya HA. 2017. Assessment of genetic diversity in crop plants-an overview. Adv. Plants Agric. Res 7(3):279-286.
Blinova IV. 2012. Intra- and interspecific morphological variation of some European terrestrial orchids along a latitudinal gradient. Russian Journal of Ecology 43(2): 111–116.
Blyton MDJ, Flanagan NS. 2012. A comprehensive guide to GenALEx 6.5: Genetic Analysis in Excel. Australian National University. http://biology.anu.edu.au/GenAlEx/
[BPS] Badan Pusat Statistik. 2020. Statistik Hortikultura. Jakarta. Badan Pusat Statistik. 104.
Cai X, Feng Z, Hou B, Xing W, Ding X. 2012. Development of microsatellite markers for genetic diversity analysis of Dendrobium loddigesii Rolfe, an endangered orchid in China. Biochemical Systematics and Ecology 43:42-47.
Chase MW, Cameron KM, Freudenstein JV, Pridgeon AM, Salazar G, van den Berg C, Schuitema, A. 2015. An updated classification of Orchidaceae. Botanical Journal of the Linnean Society 177(2): 151–174.
Chen YY, Bao ZX, Qu Y, Li W, Li ZZ. 2014. Genetic diversity and population structure of the medicinal orchid Gastrodia elata revealed by microsatellite analysis. Biochemical Systematics and Ecology 54:182-189.
Christenhusz MJM, Byng JW. 2016. The number of known plant species in the world and its annual increase. Phytotaxa 261(3): 201–217.
Collard BCY, Jahufer MZZ, Brouwer JB, Pang ECK. 2005. An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: The basic concepts. Euphytica 142:169–196.
Dong WL, Wang RN, Zhang NY, Fan WB, Fang MF, Li ZH. 2018. Molecular evolution of chloroplast genomes of orchid species: Insights into phylogenetic relationship and adaptive evolution. International Journal of Molecular Sciences 19(3): 1-20.
Dressler RL. 1993. Phylogeny and Classification of the Orchid Family. Cambridge University. Press, London. Pp 59-82.
Dwiati M, Susanto AH. 2021. Intergeneric hybridization between phalaenopsis 2166 and Vanda "saint valentine": Characterization of parents using ndhE cpDNA partial sequence. Journal of Tropical Biodiversity and Biotechnology 6(3): 1–6.
Eser E, Topcu H, Kefayati S, Sutyemez M, Islam MR, Kafkas S. 2019. Highly polymorphic novel simple sequence repeat markers from Class I repeats in walnut (Juglans regia L.). Turkish Journal of Agriculture and Forestry 43(2):174-183.
Evans A, Janssens SJH. 2021. Impact of climate change on the distribution of Sal species. Ecological Informatics 61: 1–13.
George RM, Thomas B, Uni AM. 2020. Genetic variability studies in Phalaenopsis orchids. Journal of Pharmacognosy and Phytochemistry 9(5):377-379.
Gholami S, Vafaee Y, Nazari F, Ghorbani A. 2021.(a). Molecular characterization of endangered Iranian terrestrial orchids using ISSR markers and association with floral and tuber-related phenotypic traits Molecular characterization of endangered Iranian terrestrial orchids using ISSR markers and association. Physiology and Molecular Biology of Plants, Springer 1-16.
Gholami S, Vafaee Y, Nazari F, Ghorbani A. 2021. (b). Exploring genetic variations in threatened medicinal orchids using start codon targeted (SCoT) polymorphism and marker association with seed morphometric traits. Physiology and Molecular Biology of Plants 27(4):769-85.
Givnish TJ, Spalink D, Ames M, Lyon SP, Hunter SJ, Zuluaga A, Iles WJD, Clements MA, Arroyo MTK, Leebens-Mack J, Endara L, Kriebel R, Neubig KM, Whitten WM, Williams NH, Cameron KM. 2015. Orchid phylogenomics and multiple drivers of their extraordinary diversification. Proceedings of the Royal Society B: Biological Sciences 282(1814): 1-10.
Govindaraju DR. 2019. An elucidation of over a century old enigma in genetics - Heterosis. PLoS Biol 17: e3000215 doi: 10.1371/journal. pbio.3000215
Guo YY, Luo YB, Liu ZJ. 2015. Reticulate evolution and sea-level fluctuations together drove species diversification of slipper orchids (Paphiopedilum) in South-East Asia. Mol. Ecol. 24, 2838e2855. https://doi.org/10.1111/mec.13189.
Han JE, Choi BH, Kwak M. 2018. Genetic diversity and population structure of endangered Neofinetia falcata (Orchidaceae) in South Korea based on microsatellite analysis. Journal of Species Research 7(4): 354-362.
Harniati, Jamil AS. 2020. Tingkat Keunggulan Komparatif dan Kompetitif Anggrek Indonesia di Pasar Jepang. Journal of Agriculture and Human Resources Development Studies 1(1): 18-27.
Hartati R, Widianingsih, Trianto A, Zainuri M, Ambariyanto. 2017. The abundance of prospective natural food for sea cucumber Holothuria atra at Karimunjawa Island waters, Jepara, Indonesia. Biodiversitas 18(3): 947-953.
Hartati S, Nandariyah, Yunus A, Djoar DW. 2019. Hybridization technique of black orchid (Coelogyne pandurata Lindley) to enrich the genetic diversity and to rescue the genetic extinction. Bulgarian Journal of Agricultural Science 25(4): 751–755.
Hausdorf B. 2011. Progress toward a general species concept. Evolution 65: 923–931.
Hew CS, Yong J. 2004, The Physiology of Tropical Orchids In Relation to the Industry. World Scientific, Singapore.
Hidayat T, Weston PH, Yukawa T, Ito M, Rice R. 2012. Phylogeny of subtribe Aeridinae (Orchidaceae) inferred from DNA sequences data: Advanced analyses including Australasian genera. Jurnal Teknologi (Sciences and Engineering) 59(1): 87–95.
Jiang M, Zhu Y, Wu Q, Zhang H. 2021. Complete chloroplast genome of a rare and endangered plant species Phalaenopsis zhejiangensis: genomic features and phylogenetic relationship within Orchidaceae. Mitochondrial DNA Part B 6(10):2872-2879.
Kaki A, Vafaee Y, Khadivi A. 2020. Genetic variation of Anacamptis coriophora, Dactylorhiza umbrosa, Himantoglossum affine, Orchis mascula, and Ophrys schulzei in the western parts of Iran. Industrial Crops and Products 156: https://doi.org/10.1016/j.indcrop.2020.112854.
Kang J, Lu J, Qiu S, Chen Z, Liu J, Wang H. 2015. Dendrobium SSR markers play a good role in the genetic diversity and phylogenetic analysis of Orchidaceae species. Scientia Horticulturae 183:160-166.
Kartikaningrum S, Dewanti M, Rianawati S, Pramanik D, Wegadara M. 2015. Introduksi Karakter Corak Bintik Bunga pada Persilangan Anggrek Vanda. Prosiding Seminar Nasional Perhimpunan Hortikultura, [Indonesia]. 387–393.
Khodaeiaminjan M, Kafkas S, Motalebipour EZ, Coban N. 2018. In silico polymorphic novel SSR marker development and the first SSR-based genetic linkage map in pistachio. Tree Genetics & Genomes 14(4):1-4.
Kim DG, Kim KK, Been CG. 2015. Development of intergeneric hybrids between wind orchids (Sedirea japonica and Neofinetia falcata) and moth orchids (Phalaenopsis alliances). Horticulture Environment and Biotechnology 56(1): 67–78.
Kim YK, Jo S, Cheon SH, Kwak M, Kim YD, Kim KJ. 2020. Plastome evolution and phylogeny of subtribe Aeridinae (Vandeae, Orchidaceae). Molecular Phylogenetics and Evolution 1(144):106721.
Kotilinek M, Tesitelova T, Kosnar J, Fibich P, Hemrova L, Koutecky P, Munzbergova Z, Jersakova J. 2020. Seed dispersal and realized gene flow of two forest orchids in a fragmented landscape. Plant Biology 22(3):522-532.
Kumar M, Vallabhbhai, Patel S, Chaudhary V, Rakesh S, CSIR-NBRI V, Pratap Marg R, Pradesh U, Ujjwal Sirohi I, Singh J, Mukesh Kumar C, Rakesh Sharma V, Sirohi U. 2018. Advances in biochemical and molecular marker techniques and their applications in genetic studies of orchid: A review. International Journal of Chemical Studies 6(6): 806–822.
Kurniawati J, Sugiyarto L, Yulianti E, Nurcahyo H, Mercuriani IS. 2019. Molecular Identification of Several Orchid Species Based on OPA10 and OPA18 RAPD Marker. Journal of Physics: Conference Series 1397(1): 1-5.
Lamichhane S, Thapa S. 2022. Advances from Conventional to Modern Plant Breeding Methodologies. Plant Breeding and Biotechnology 10(1):1-4.
Lee HJ, Park HR, Lee AJ, Nam DE, Lee DG, Do Y, Chung KW. 2020. Genetic authentication of cultivars with flower-variant types using SSR markers in spring orchid, Cymbidium goeringii. Horticulture, Environment, and Biotechnology n61(3):577-90.
Lema M. 2018. Marker-assisted selection in comparison to conventional plant breeding. Agricultural Research and Technology 14:555914.
Lim A, Yue GH, Lim-Ho CL, Hong Y. 2007. Development and characterization of microsatellites in Vanda varieties: Primer note. Molecular Ecology Notes 7(3): 461–463.
Liu K, SV Muse. 2005. Power marker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128-2129.
Liu WL, Shih HC, Weng IS, Ko YZ, Tsai CC, Chou CH, Chiang YC. 2016. Characterization of Genomic Inheritance of Intergeneric Hybrids between Ascocenda and Phalaenopsis Cultivars by GISH, PCR-RFLP, and RFLP. PLOS One 11(4). Doi. https://doi.org/10.1371/journal.pone.0153512.
Lynch MM, Milligan BG. 1994. Analysis of population genetic structure with RAPD markers. Molecular Ecology 3(2):91-99.
Martel C, Scopece G, Cozzolino S, Ayasse M, Pinheiro F, Cafasso D. 2021. Genetic diversity in natural populations of the endangered Neotropical orchid Telipogon peruvianus. Plant Species Biology 36(1):6-16.
Marwoto B, Badriah DS, Dewanti M dan Sanjaya L. 2012. Persilangan Interspesifik dan Intergenerik Anggrek Phalaeonopsis untuk Menghasilkan Hibrid Tipe Baru. Prosiding Seminar Nasional Anggrek. 101-116.
Minasiewicz J, Znaniecka JM, Górniak M, Kawi?ski A. 2018. Spatial genetic structure of an endangered orchid Cypripedium calceolus (Orchidaceae) at a regional scale: limited gene flow in a fragmented landscape. Conservation Genetics 19(6):1449-1460.
Morales M, Ackerman JD, Tremblay RL. 2010. Morphological flexibility across an environmental gradient in the epiphytic orchid, Tolumnia Variegata: Complicating patterns of fitness. Botanical Journal of the Linnean Society 163: 431–446.
Mutakabbir AE, Duakaju NN. 2019. Analisis Kelayakan Finansial Usaha Tanaman Hias di Kota Samarinda. Jurnal Agribisnis Komunikasi Pertanian 2(1): 25-34.
Nakahama N, Asai T, Matsumoto S, Suetsugu K, Kurashima O, Matsuo A, Suyama Y. 2021. Detection and dispersal risk of genetically disturbed individuals in endangered wetland plant species Pecteilis radiata (Orchidaceae) in Japan. Biodiversity and Conservation 30(6):1913-1927.
Niu Z, Xue Q, Zhu S, Sun,J, Liu W, Ding X. 2017. The complete plastome sequences of four orchid species: Insights into the evolution of the Orchidaceae and the utility of plastomic mutational hotspots. Frontiers in Plant Science 8(5): 1–11.
Noh SW, Park JK, Yu JS, Nam DE, Di Y, Chung KW. 2020. Genetic Diversity and Population Structure of the Spring Orchid Cymbidium goeringii in Korean Distant Islands. Diversity 12(486): 1-10 doi:10.3390/d12120486.
Nugroho FA, Sobir, Matra DD. Analisis Kekerabatan Progeni Hasil Persilangan Durio zibethinus Murr x Durio kutejensis Taman Buah Mekarsari berdasarkan Karakter Vegetatif dan Marka Mikrosatelit. Prosiding Seminar Nasional Perhimpunan Hortikultura, 2015 [Indonesia].
Pandey M, Sharma J. Efficiency of microsatellite isolation from orchids via next-generation sequencing.
Parmadi, Emilia, Zulgani. 2018. Daya Saing Produk Unggulan Sektor Pertanian Indonesia Dalam Hubungannya Dengan Pertumbuhan Ekonomi. Jurnal Paradigma Ekonomika 13(2): 77-86.
Peakall R, Smouse PE. 2012. GenALEx 6.5: Genetic Analysis in Excel. Population Genetic Software for Teaching and Research-an Update. Bioinformatics 28(19): 2537–39.
Perrier X, Jacquemoud-Collet JP. 2014. DARwin software: Dissimilarity analysis and representation for windows. http://darwin.cirad_fr/darwin.
Peyachoknagul S, Nettuwakul C, Phuekvilai P, Wannapinpong S, Srikulnath K. 2014. Development of microsatellite markers of Vandaceous orchids for species and variety identification. Genetics and Molecular Research 13(3): 5441–5445.
Phuekvilai P, Pongtongkam P, Peyachoknagul S. 2009. Development of microsatellite markers for Vanda orchid. Kasetsart Journal - Natural Science 43(3): 497–506.
Puspitasari, Nurmalina R, Fariyanti A,Kiloes AM. 2018. Pengaruh Faktor Internal dan Eksternal Terhadap Perilaku Kewirausahaan dan Dampaknya Terhadap Kinerja Usaha Petani Anggrek. Jurnal Hortikultura 28(2): 299-310.
Rajaram MC, Yong CS, Gansau JA, Go R. 2019. DNA barcoding of endangered Paphiopedilum species (Orchidaceae) of Peninsular Malaysia. Phytotaxa 387(2):94-104.
Sambrook J, Fritsch E, Maniathis T. 1989. Molecular Cloning. Cold Spring Harbor Lab. Press, New York.The USA.
Sayifullah, Emmalian. 2018. Pengaruh Tenaga Kerja Sektor Pertanian dan Pengeluaran Pemerintah Sektor Pertanian Terhadap Produk Domestik Bruto Sektor Pertanian di Indonesia. Jurnal Ekonomi-Qu (Jurnal Ilmu Ekonomi) 8(1):66-81.
Shi Y, Yang L, Yang Z, Ji Y. 2018. The complete chloroplast genome of Pleione bulbocodioides (Orchidaceae). Conservation Genetics Resources 10(1): 21–25.
Suni SS. 2017. Dispersal of the orchid bee Euglossa imperialis over degraded habitat and intact forest. Conservation Genetics. 18(3):621-630.
Surveswaran S, Gowda V, Sun M. 2018. Using an integrated approach to identify cryptic species, divergence patterns, and hybrid species in Asian ladies' tresses orchids (Spiranthes, Orchidaceae). Molecular Phylogenetics and Evolution 124(2): 106–121.
Swarup S, Cargill EJ, Crosby K, Flagel L, Kniskern J, Glenn KC. 2021. Genetic diversity is indispensable for plant breeding to improve crops. Crop Science 61(2):839-52.
Tang CY and Chen WH. 2007. Breeding and Development of New Varieties in Phalaenopsis. Orchid Biotechnology. 1-22.
Tang H, Tang L, Shao S, Peng Y, Li L, Luo Y. 2021. Chloroplast genomic diversity in Bulbophyllum section Macrocaulia (Orchidaceae, Epidendroideae, Malaxideae): Insights into species divergence and adaptive evolution. Plant diversity 43(5):350-61.
Tikendra L, AmomT, Nongdam P. 2019. Molecular genetic homogeneity assessment of micro-propagated Dendrobium moschatum Sw. - A rare medicinal orchid using RAPD and ISSR markers. Plant Gene 19 (100196):1-12.
Wegadara M, Thamrin M, Kartikaningrum S, Marwoto B, Sukma D, Sudarsono S. 2022. Morphological and flower phenotype diversities of Vanda orchid accessions as breeding material at IOCRI, Indonesia. Acta Horticulturae 1334: 119-126.
Xie C, Xu S. 1998. Efficiency of multistage marker-assisted selection in the improvement of multiple quantitative traits. Heredity 80(4): 489–498.
Yun SA, Son HD, Im HT, Kim SC. 2020. Genetic diversity and population structure of the endangered orchid Pelatantheria scolopendrifolia (Orchidaceae) in Korea. PLOS ONE8(13):1-21 https://doi.org/10.1371/journal.pone.0237546.
Zameda Igga EBK, Harisudin M, Sundari MT. 2019. Analisis Pendapatan Usaha Penjualan Tanaman Hias di Kota Surakarta. AGRISTA 7(1): 22-31.
Zhang Y, Zhang X, Chen X, Sun W, Li J. 2018. Genetic diversity and structure of tea plant in Qinba area in China by three types of molecular markers. Hereditas 155(22):1-12.
Zhang SB, Chen WY, Huang JL, Bi YF, Yang XF. 2015. Orchid species richness along elevational and environmental gradients in Yunnan, China. PLoS ONE 10(11): 1–23.
Zhang, Y.X., Zeng, C.X., Li, D.Z., 2012. Complex evolution in Arundinarieae (Poaceae: Bambusoideae): incongruence between plastid and nuclear GBSSI gene phylogenies. Mol. Phylogenet. Evol. 63, 777e797.
Zhao TM, Zheng SG, Hu YD, Zhao RX, Li HJ, Zhang XQ, Chun Z. 2019. Classification of interspecific and intraspecific species by genome-wide SSR markers on Dendrobium. South African Journal of Botany 127:136-46.
Zhou S, Zhou XX, Jin Y, Schuiteman A, Kumar P, Yuan D, Tian HZ. 2021. Goodyera araneosa: a new species of Orchidaceae from Sichuan, China, and its affinities. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology 155(2):344-349.
Zotz G, Hietz P, Schmidt G. 2001. Small plants, large plants: The importance of plant size for the physiological ecology of vascular epiphytes. Journal of Experimental Botany 52(363): 2051–2056.
Zotz G. 2013. The systematic distribution of vascular epiphytes-a critical update. Botanical Journal of the Linnean Society 171(3): 453–481.
Zou LH, Huang JX, Zhang GQ, Liu ZJ, Zhuang XY. 2015. A molecular phylogeny of Aeridinae (Orchidaceae: Epidendroideae) inferred from multiple nuclear and chloroplast regions. Molecular Phylogenetics and Evolution 85:247-254.
Most read articles by the same author(s)
- DEWI SUKMA, ALINE SISI HANDINI, SUDARSONO, Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 11 (2020)
- BARAHIMA ABBAS, YANUARIUS RENWARIN, MUHAMMAD HASIM BINTORO, SUDARSONO SUDARSONO, MEMEN SURAHMAN2, HIROSHI EHARA, Genetic diversity of sago palm in Indonesia based on chloroplast DNA (cpDNA) markers , Biodiversitas Journal of Biological Diversity: Vol. 11 No. 3 (2010)
- IZZATUL MUHALLILIN, SYARIFAH IIS AISYAH, DEWI SUKMA, The diversity of morphological characteristics and chemical content of Celosia cristata plantlets due to gamma ray irradiation , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 3 (2019)
- JEKKI IRAWAN, DINY DINARTI, SUDARSONO, AWANG MAHARIJAYA, Flowering ability and expression of the shLFY (shallot-LFY) gene in several Indonesian shallot (Allium cepa, aggregatum group) varieties , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 12 (2021)
- LALU FIRMAN BUDIMAN, ARDHA APRIYANTO, ADI PANCORO, SUDARSONO SUDARSONO, Genetic diversity analysis of Tenera × Tenera and Tenera × Pisifera Crosses and D self of oil palm (Elaeis guineensis) parental populations originating from Cameroon , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 4 (2019)
- LUKMAN, DINY DINARTI, ULFAH JUNIARTI SIREGAR, MAMAN TURJAMAN, SUDARSONO, Isolation and molecular identification of agarwood-inducing fungi and their virulence test using Aquilaria sp. seedlings , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 1 (2023)
- LUKMAN, DINY DINARTI, ULFAH JUNIARTI SIREGAR, MAMAN TURJAMAN, SUDARSONO, Characterization and identification of agarwood-producing plants (Aquilaria spp.) from North Aceh, Indonesia, based on morphological and molecular markers , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 9 (2022)
- AHMAD RIDUAN, DJOKO SANTOSO, SUDARSONO, Response of transgenic tobacco with P5CS gene expression to polyethylene glycol-induced drought stress , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 10 (2024)
- AZIS NATAWIJAYA, SINTHO W ARDIE, MUHAMAD SYUKUR, ISMAIL MASKROMO, ALEX HARTANA, SUDARSONO SUDARSONO, Genetic structure and diversity between and within African and American oil palm species based on microsatellite markers , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 5 (2019)
- ANNISAA RAHMAWATI, DINY DINARTI, ISMAIL MASKROMO, HUGO ALFRIED VOLKAERT, SUDARSONO, Coconut diversity based on chloroplast Single Nucleotide Amplified Polymorphism (SNAP) and Insertion-Deletion (InDel) markers , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 8 (2022)