An orange pigment from the marine bacterium Paracoccus haeundaensis SAB E11 as a prospective source of natural antioxidants

##plugins.themes.bootstrap3.article.sidebar##

PDF
DOI https://doi.org/10.13057/biodiv/d230940
Issue
Vol. 23 No. 9 (2022)
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

##plugins.themes.bootstrap3.article.main##

HERMAWATY ABUBAKAR
Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor. Jl. Raya Dramaga, Bogor 16680, West Java, Indonesia
RIKA INDRI ASTUTI
Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor. Jl. Raya Dramaga, Bogor 16680, West Java, Indonesia
SRI LISTYOWATI
Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor. Jl. Raya Dramaga, Bogor 16680, West Java, Indonesia
IRMANIDA BATUBARA
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor. Jl. Raya Dramaga, Bogor 16680, West Java, Indonesia
ARIS TRI WAHYUDI ♥
Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor. Jl. Raya Dramaga, Bogor 16680, West Java, Indonesia

Abstract

Abstract. Abubakar H, Astuti RI, Listyowati S, Batubara I, Wahyudi AT. 2022. An orange pigment from the marine bacterium Paracoccus haeundaensis SAB E11 as a prospective source of natural antioxidants. Biodiversitas 23: 4730-4737. Bacterial pigment extracts are the source of many natural antioxidant substances. The present study aimed to assess the antioxidant activity of an orange pigment derived from a marine bacterium, identified as Paracoccus haeundaensis SAB E11, analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis(ethylbenzothiazoline-6-sulfonic acid) (ABTS). Methanol-pigmented crude extract of P. haeundaensis SAB E11 exhibited the best DPPH scavenging activity with an IC50 value of 54.7 mg/mL, while the n-hexane crude pigment extract of the bacterium reduced ABTS optimally with an IC50 value of 24.46 mg/mL. The analysis of antioxidant compounds in P. haeundaensis SAB E11 was identified by combining liquid chromatography with tandem mass spectrometry (LCMS/MS). The five antioxidant compounds from methanol and n-hexane crude pigment extract of P. haeundaensis SAB E11 have been detected. Polyphenols were found to be the major compound, such as 3,5,3’,4’-tetrahydroxystilbene, scopoletin, and liquiritigenin. The other compounds were dihydroactinidiolide (terpenes) and ricinoleic acid (a fatty acid compound). The orange-colored extract of marine bacterium P. haeundaensis SAB E11 could be promising pharmacological capabilities as an antioxidant.

##plugins.themes.bootstrap3.article.details##

References
Abubakar H, Yuhana M, Wahyudi AT. 2011. Skrining bakteri yang berasosiasi dengan spons Jaspis sp. sebagai penghasil senyawa antimikroba. Ilmu Kelautan: Indones J Mar Sci 16(1): 35–40. DOI: 10.14710/ik.ijms.16.1.35-40).
Ali SS, Ahsan H, Zia MK, Siddiqui T, Khan FH. 2020. Understanding oxidants and antioxidants: classical team with new players. J Food Biochem 44(3): 1–13. DOI: 10.1111/jfbc.13145.
Allahkarami S, Akhavan SA, Hosseini H, Razavi MR. 2021. Isolation and identification of carotenoid-producing Rhodotorula sp. from Pinaceae forest ecosystems and optimization of in vitro carotenoid production. Biotechnol Rep 32: 1–12. DOI: 10.1016/j.btre.2021.e00687.
Andrés JC, Manuel PLJ, Plou FJ, Pérez-Lebeña E, Reinbothe S. 2021. Molecular sciences the chemistry of reactive oxygen species (ROS) revisited: outlining their role in biological macromolecules (DNA, lipids and proteins) and induced pathologies. Int J Mol Sci 22(4642): 1–21. DOI: 10.3390/ijms22094642.
Batubara I, Komariah K, Sandrawati A, Nurcholis W. 2020. Genotype selection for phytochemical content and pharmacological activities in ethanol extracts of fifteen types of Orthosiphon aristatus (Blume) Miq. leaves using chemometric analysis. Sci Rep 10: 1–11. DOI: 10.1038/s41598-020-77991-2.
Das M, Devi KP. 2021. Dihydroactinidiolide regulates Nrf2/HO-1 expression and inhibits caspase-3/Bax pathway to protect SH-SY5Y human neuroblastoma cells from oxidative stress induced neuronal apoptosis. NeuroToxicology 84: 53–63. DOI: 10.1016/j.neuro.2021.02.006.
di Meo S, Venditti P. 2020. Evolution of the knowledge of free radicals and other oxidants. Oxid Med Cell Long 2020: 1–32. DOI: 10.1016/j.bmc.2010.11.016.
Furuya T, Imaki N, Shigei K, Sai M, Kino K. 2019. Isolation and characterization of Gram-negative and Gram-positive bacteria capable of producing piceatannol from resveratrol. Appl Microbiol Biotechnol 103(14):5811–5820. DOI: 10.1007/s00253-019-09875-z.
Genç Y, Bardakci H, Yücel Ç, Karatoprak G?, Akkol EK, Barak TH, Sobarzo-Sánchez E. 2020. Oxidative stress and marine carotenoids: application by using nanoformulations. Mar Drugs 18(423): 1–32. DOI: 10.3390/MD18080423.
Gnonlonfin GJB, Sanni A, Brimer L. 2012. Review scopoletin - a coumarin phytoalexin with medicinal properties. Crit Rev Plant Sci 31(1): 47–56. DOI: 10.1080/07352689.2011.616039.
Hamid HA, Kupan S, Yusoff MM. 2017. Dihydroactinidiolide from thermal degradation of ?-carotene. Int J Food Prop 20(3):674–680. DOI: 10.3390/md18010028.
Hamidi M, Kozani Pouya Safarzadeh, Kozani Pooria Safarzadeh, Pierre G, Michaud P, Delattre C. 2019. Marine bacteria versus microalgae: who is the best for biotechnological production of bioactive compounds with antioxidant properties and other biological applications? Mar Drugs 18(28): 1–38. DOI: 10.3390/md18010028.
Kaur P, Ghoshal G, Jain A. 2019. Bio-utilization of fruits and vegetables waste to produce ?-carotene in solid-state fermentation: characterization and antioxidant activity. Process Biochem 76: 155–164. DOI: 10.1016/j.procbio.2018.10.007.
Lee JH, Kim YS, Choi TJ, Lee WJ, Kim YT. 2004. Paracoccus haeundaensis sp. nov., a Gram-negative, halophilic, astaxanthin-producing bacterium. Int J Syst Evol Microbiol 54(5):1699–1702. DOI: 10.1099/ijs.0.63146-0.
Liu H, Zhang C, Zhang X, Tan K, Zhang H, Cheng D, Ye T, Li S, Ma H, Zheng H. 2020. A novel carotenoids-producing marine bacterium from noble scallop Chlamys nobilis and antioxidant activities of its carotenoid compositions. Food Chem 320:1–9. DOI: 10.1016/j.foodchem.2020.126629.
Manohara RSA, Mudgal J, Bansal P, Vasanthraju SG, Srinivasan KK, Rao CM, Gopalan Kutty N. 2011. Antioxidant, anti-inflammatory and anti-hyperglycaemic activities of heterocyclic homoprostanoid derivatives. Bioorg Med Chem 19(1):384–392. DOI: 10.1016/j.bmc.2010.11.016.
Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ, Wade WG. 1998. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 64(2):795–799. DOI:10.1128/aem.64.2.795-799.1998.
Milke L, Aschenbrenner J, Marienhagen J, Kallscheuer N. 2018. Production of plant-derived polyphenols in microorganisms: current state and perspectives. Appl Microbiol Biotechnol 102(4): 1575–1585. DOI: 10.1007/s00253-018-8747-5.
Nandi A, Yan LJ, Jana CK, Das N. 2019. Role of catalase in oxidative stress- and age-associated degenerative diseases. Oxid Med Cell Long 2019: 1–19. DOI: 10.1155/2019/9613090.
Osamudiamen PM, Oluremi BB, Oderinlo OO, Aiyelaagbe OO. 2020. Trans-resveratrol, piceatannol and gallic acid: potent polyphenols isolated from Mezoneuron benthamianum effective as anticaries, antioxidant and cytotoxic agents. Sci Afr 7: 1–7. DOI: 10.1016/j.sciaf.2019.e00244.
Pabi? S, Kula J. 2016. Synthesis and bioactivity of (R)-ricinoleic acid derivatives: a review. Curr Med Chem 23(35): 4037–4056. DOI: 10.2174/0929867323666160627104453.
Patil MP, Kang M jae, Niyonizigiye I, Singh A, Kim JO, Seo YB, Kim G do. 2019. Extracellular synthesis of gold nanoparticles using the marine bacterium Paracoccus haeundaensis BC74171T and evaluation of their antioxidant activity and antiproliferative effect on normal and cancer cell lines. Colloids Surf B: Biointerfaces 183: 1–7. DOI: 10.1016/j.colsurfb.2019.110455.
Pérez-gálvez A, Viera I, Roca M. 2020. Carotenoids and chlorophylls as antioxidants. Antioxidants 9(6):1–39. DOI: 10.3390/antiox9060505. DOI: 0.3390/md20010073.
Picot L, Mouget J-L, Wook Jeong S, Eun Yang J, Jun Choi Y. 2022. Isolation and characterization of a yellow xanthophyll pigment-producing marine bacterium, Erythrobacter sp. SDW2 strain, in Coastal Seawater. Mar Drugs 20(73): 1–10. DOI: 0.3390/md20010073.
Prastya ME, Astuti RI, Batubara I, Wahyudi AT. 2018. Bacillus sp. SAB E-41-derived extract shows antiaging properties via ctt1-mediated oxidative stress tolerance response in yeast Schizosaccharomyces pombe. Asian Pac J Trop Biomed 8(11): 533–539. DOI: 10.4103/2221-1691.245958.
Ramesh C, Vinithkumar NV, Kirubagaran R, Venil CK, Dufossé L. 2019. Multifaceted applications of microbial pigments: current knowledge, challenges and future directions for public health implications. Microorganisms 7(7): 1–46. DOI: 10.3390/microorganisms7070186.
Reis-Mansur MCPP, Cardoso-Rurr JS, Silva JVMA, de Souza GR, Cardoso V da S, Mansoldo FRP, Pinheiro Y, Schultz J, Lopez Balottin LB, da Silva AJR, et al. 2019. Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01. Sci Rep 9(1): 1–14. DOI: 10.1038/s41598-019-45840-6.
Rudra A, Arvind I, Mehra R. 2021. Polyphenols: types, sources and therapeutic applications. Int J Home Sci 7(3): 69–75. DOI: 10.22271/23957476.2021.v7.i3a.1182.
Sharma R, Ghoshal G. 2020. Optimization of carotenoids production by Rhodotorula mucilaginosa (MTCC-1403) using agro-industrial waste in bioreactor: a statistical approach. Biotechnol Rep 25: 1–11. DOI: 10.1016/j.btre.2019.e00407.
Shaw CY, Chen CH, Hsu CC, Chen CC, Tsai YC. 2003. Antioxidant properties of scopoletin isolated from Sinomonium acutum. Phytother Res 17(7): 823–825. DOI: 10.1002/ptr.1170.
Shi C, Wu H, Xu K, Cai T, Qin K, Wu L, Cai B. 2020. Liquiritigenin-loaded submicron emulsion protects against doxorubicin-induced cardiotoxicity via antioxidant, anti-Inflammatory, and anti-apoptotic activity. Int J Nanomed 15: 1101–1115. DOI: 10.2147/IJN.S23583.