Analysis of phytochemical constituents and antioxidant activity from the fractions of Luvunga sarmentosa root extract using LCMS/MS
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Abstract
Abstract. Syarpin, Permatasari S, Pujianto DA. 2023. Analysis of phytochemical constituents and antioxidant activity from the fractions of Luvunga sarmentosa root extract using LCMS/MS. Biodiversitas 24: 733-740. Luvunga sarmentosa, one of the native plants of Central Kalimantan, has been used by Dayak people, especially its root, to increase sexual activity, cure sexual dysfunction and increase male fertility. Dayak people boil the stems and roots of L. sarmentosa and drink the extract. However, studies on L. sarmentosa are still limited to phytochemical content and bioactivity screening of the root extract of L. sarmentosa. This research aimed to determine the phytochemical content and antiradical activities of L. sarmentosa root fractions. Fractions were obtained using solvents with different polarities. The sample was extracted by maceration using ethanol, and the extract was fractionated by column chromatography using hexane, chloroform, ethyl acetate, and methanol. The chemical content of each fraction was identified using LCMS/MS. The antiradical activity was performed against DPPH using a UV-Visible spectrophotometer. The LCMS/MS analysis results indicated that each fraction contained different percentages of compounds, such as alkaloids, terpenoids, phenylpropanoids, stilbenes, flavonoids, tannins, fatty acids, and steroids. Ostruthin was a compound detected in all fractions with a retention time of 20,917 minutes and was considered a marker compound from the roots of L. sarmentosa. The antiradical activity assay showed that the methanol fraction had the best activity with an IC50 value of 83.42 ppm. The IC50 data shows that increasing the percentage of alkaloid compounds increased their antioxidant activity.
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References
An TC, Ngoc PH, Sang PM, Truc PN, Tien NHT, Nhu TPH, Tam PTT, Hung LN, Trung PV. 2021. Directly Purification of Ostruthin from Paramignya trimera roots by CPC (centrifugal partition chromatography). Vietnam J. Chem., 59(1): 69-72. DOI: 10.1002/vjch.202000117
Andiriyani MM, Untari EK, Wahdaningsih S. 2014. Pengaruh pemberian ekstrak etanol daun bawang mekah (Eleutherine Americana Merr.) terhadap kadar malondialdehide tikus wistar jantan pasca paparan asap rokok [Effect of giving ethanol extract of leek mecca (Eleutherine Americana Merr.) on malondialdehyde levels of male wistar rats after exposure to cigarette smoke]. Jurnal Fitofarmaka Indonesia 1(2): 43-50. DOI: 10.33096/jffi.v1i2.189
Anggriani MF. 2018. Identifikasi dan Potensi Senyawa Metabolit Sekunder Pada Akar Tumbuhan Saluang Belum (Lavanga sarmentosa (Blume) Kurz) Sebagai Afrodisiak [Identification and Potential of Secondary Metabolite Compounds in the Roots of Saluang yet (Lavanga sarmentosa (Blume) Kurz) as Aphrodisiac]. J Ilm Kanderang Tingang 9(1): 97–102. DOI: 10.37304/jikt.v9i1.9
Dang PH, Dao THX, Le VT, Nguyen CM, Ly TT, Nguyen HX, Le TH, Do TNV, Nguyen MTT, Sun S, Awale S, Nguyen NT. 2020. Synthesis of Alkyl Triphenylphosphonium Ostruthin Derivatives as Potential Cytotoxic Candidates. ChemistrySelect 5(41): 12636–12640. DOI:10.1002/slct.202003152
Delgado-Povedano MM, Sánchez-Carnerero CC, Priego-Capote F, Ferreiro-Vera C. 2019. Untargeted characterization of extracts from Cannabis sativa L. cultivars by gas and liquid chromatography coupled to mass spectrometry in high resolution mode. Talanta: 1-10. DOI:10.1016/j.talanta.2019.120384
Fauzi, Widodo H. 2019. Short communication: Plants used as aphrodisiacs by the Dayak ethnic groups in Central Kalimantan, Indonesia. Biodiversitas 20(7):1859–1865. DOI: 10.13057/biodiv/d200710
Gan J, Feng Y, He Z, Li X, Zhang H. 2017. Correlations between Antioxidant Activity and Alkaloids and Phenols of Maca (Lepidium meyenii). Journal of Food Quality: 1–10. DOI:10.1155/2017/3185945
Gul R, Jan SU, Faridullah S, Sherani S, Jahan N. 2017. Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan. The Scientific World Journal: 1–7. DOI:10.1155/2017/5873648
Gutiérrez DMA, Bah M, Garduño MLR, Mendoza SOM, Serrano VC. 2014. Anti-Inflammatory and Antioxidant Activities of Methanol Extracts and Alkaloid Fractions of four Mexican Medicinal Plants of Solanaceae. African Journal of Traditional, Complementary and Alternative Medicines 11(3): 259–267. DOI:10.4314/ajtcam.v11i3.36
Harborne, J.B. 1987. Metode Fitokimia. Edisi ke-2 [Phytochemical Method. 2nd Edition]. Padmawinata K, Soediro I. Institut Teknologi Bandung, Bandung.
Jannat B, Ghorbani K, Kouchaki S, Sadeghi N, Eslamifarsani E, Rabbani F, Beyramysoltan S. 2020. Distinguishing tissue origin of bovine gelatin in processed products using LC/MS technique in combination with chemometrics tools. Food Chemistry 319: 1–6. DOI: 10.1016/j.foodchem.2020.126302
Joseph A, Thuy TTT, Thanh LT, Okada M, Biagini G. 2018. Antidepressive and anxiolytic effects of ostruthin, a TREK-1 channel activator. PLOS ONE 13(8): 1-19. DOI: 10.1371/journal.pone.0201092
Lee J, Krotulski AJ, Fogarty MF, Papsun DM, Logan BK. 2019. Chromatographic separation of the isobaric compounds cyclopropylfentanyl, crotonylfentanyl, methacrylfentanyl, and para-methylacrylfentanyl for specific confirmation by LC-MS/MS. Journal of Chromatography B 1118-1119: 164-170. DOI:10.1016/j.jchromb.2019.04.033
Liu C, Kao C, Wu H, Li W, Huang C, Li H, Chen C. 2014. Antioxidant and Anticancer Aporphine Alkaloids from the Leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena. Molecules, 19(11), 17829–17838. DOI:10.3390/molecules191117829
Ludwiczuk, A. Skalicka-Wo´zniak K and Georgiev M.I. (2017). Terpenoids. Elsevier, Amsterdam.
Manosalva L, Mutis A, Urzúa A, Fajardo V, Quiroz A. 2016. Antibacterial Activity of Alkaloid Fractions from Berberis microphylla G. Forst and Study of Synergism with Ampicillin and Cephalothin. Molecules 21(1), 76–. DOI:10.3390/molecules21010076
Mardawati E, Filianty F dan Harta H. 2008. Kajian Aktivitas Antioksidan Ekstrak Kulit Manggis (Garcinia mangostana L.) dalam Rangka Pemanfaatan Limbah Kulit Manggis di Kecamatan Puspahiang Kabupaten Tasikmalaya [Study of Antioxidant Activity of Mangosteen Peel Extract (Garcinia mangostana L.) in the Context of Utilizing Mangosteen Peel Waste in Puspahiang District, Tasikmalaya Regency]. Jurnal Industri Teknologi Pertanian,
Musfirah Y, Bachri MS, Nurani LH. 2016. Efek Ekstrak Etanol 70 % Akar Saluang Balum ( Lavanga sarmentosa , Blume kurz ) Terhadap Spermatogenesis dan Gambaran Histopatologik Testis Mencit [Effects of Ethanol Extract 70% Saluang Balum Root (Lavanga sarmentosa, Blume kurz) Against Spermatogenesis and Histopathological Appearance of Mice Testis]. J. Pharmascience 03(02): 131–41. DOI : 10.20527/jps.v3i2.5748
Namdar D, Mazuz M, Ion A, Koltai H. 2018. Variation in the compositions of cannabinoid and terpenoids in Cannabis sativa derived from inflorescence position along the stem and extraction methods. Industrial Crops and Products 113: 376–382. DOI:10.1016/j.indcrop.2018.01.060
Nugraha AS, Damayanti YD, Wangchuk P, Keller PA. 2019. Anti-Infective and Anti-Cancer Properties of the Annona Species: Their Ethnomedicinal Uses, Alkaloid Diversity, and Pharmacological Activities. Molecules 24(23): 1-31. DOI:10.3390/molecules24234419
Stacey G, Birchler J, Ecker J, Martin CR. Stitt M, Zhou J. 2016. Extraction and Analysis of Terpenes/Terpenoids. Current Protocols in Plant Biology 1: 345–358. DOI:10.1002/cppb.20024
Syarif YM, Bachri MS, Nurani LH. 2016. Potensi ekstrak etanol 70% akar saluang balum (Lavanga sarmentosa blume kurz) terhadap kualitas dan viabilitas sperma mencit [Potential 70% ethanol extract of saluang balum root (Lavanga sarmentosa blume kurz) on sperm quality and viability of mice]. Pharmaciana 6(2):131–138. DOI: 10.12928/pharmaciana.v6i2.4037
Vinaixa M, Schymanski EL, Neumann S, Navarro M, Salek RM, Yanes O. 2015. Mass spectral databases for LC/MS and GC/MS-based metabolomics: state of the field and future prospects. Trends in Analytical Chemistry: 1-33. DOI: 10.1016/j.trac.2015.09.005
Vo NT, Kusagawa E, Nakano K, Moriwaki C, Miyake Y, Haruyama S, Fukuhara S, Nguyen NT, Dang PH, Nguyen MTT, Kataoka T. 2021. Biological Evaluation of Alkyl Triphenylphosphonium Ostruthin Derivatives as Potential Anti-Inflammatory Agents Targeting the Nuclear Factor ?B Signaling Pathway in Human Lung Adenocarcinoma A549 Cells. BioChem. 1(2):107-121. DOI: 10.3390/biochem1020010
Wati H, Muthia R, Jumaryatno P, Hayati F, August J. 2018. Screening And Aphrodisiac Activity Of Luvunga Sarmentosa Kurz Ethanol Extract In Male Wistar Albino Rats. Research J. Pharm. Biol. Chem. Sci. 9 (931): 931–937.
Widarta IWR, Wiadnyani AAIS. 2019. Effect of Drying Methods on the Antioxidant Activity of Avocado Leaves. Jurnal Aplikasi Teknologi Pangan 8 (3): 80 – 85. DOI : 10.17728/jatp.3361
Yunita E, Kurniati T, Rosa FL, Melati P, Lestari N. 2022. Short Communication: Analysis of detected metabolites compounds from the crude extract of Rimau Gerga Lebong oranges fruit (Citrus reticulata ‘RGL’) using LC-QTOF-MS/MS. Biodiversitas 23 (7): 3778-3783. DOI: 10.13057/biodiv/d230754
Zhang D, Wan Y, Hao J, Hu R, Chen C, Yao X, Zhao W, Liu Z, Li L. 2018. Evaluation of the alkaloid, polyphenols, and antioxidant contents of various mulberry cultivars from different planting areas in eastern China. Industrial Crops and Products 122: 298–307. DOI: 10.1016/j.indcrop.2018.05.065