Diversity and antioxidant activity of endophytic fungi isolated from salam (Syzygium polyanthum)
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Abstract. Widjajanti H, Elfita, Sari MT, Hidayati N, Hariani PL, Setiawan A. 2023. Diversity and antioxidant activity of endophytic fungi isolated from salam (Syzygium polyanthum). Biodiversitas 24: 3051-3062. Salam (Syzygium polyanthum (Wight) Walp.) is a medicinal plant from the family Myrtaceae. The leaves are used as a spice and treat diseases such as diabetes mellitus, hypertension, ulcers, diarrhea, gastritis, and skin. This study aimed to determine endophytic fungi's diversity and antioxidant activity in the S. polyanthum plant. In addition, the distribution of endophytic fungi found on S. polyanthum was compared to that of previously reported endophytic fungi on other species of Syzygium. The distribution pattern of these endophytic fungi is needed to determine the presence of potential endophytic fungi that can live in host plants of the same genus. The endophytic fungi were isolated from fresh root bark, stem bark, and leaf tissues of S. polyanthum. The endophytic fungi were identified morphologically to determine their level of diversity; their extract was then tested for their antioxidant activity using the DPPH method. The extract that showed the highest antioxidant activity was identified molecularly. A total of 18 endophytic fungi were obtained from S. polyanthum, including seven isolates from root bark (HSA1–HSA7), four isolates from stem bark (HSB1–HSB4), and seven isolates from leaves (HSD1–HSD7). The morphological identification showed seven genera scattered in all investigated parts: Trichoderma, Penicillium, Aspergillus, Pythium, Papulaspora, Pythiogeton, and Clonostachys. The HSD5 isolate showed the best antioxidant activity, and the molecular identification confirmed this isolate as Clonostachys rosea. The comparison of the distribution of endophytic fungi isolated from S. polyanthum to endophytic fungi isolated from the host genus Syzygium showed that endophytic fungi of three genera, namely Clonostachys, Papulaspora, and Pythiogeton, are specifically found on S. polyanthum. In addition, those of four other genera, namely Trichoderma, Aspergillus, Penicillium, and Pythium, are found on other species of Syzygium.
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References
Abbas S, Shanbhag T, Kothare A. 2021. Applications of bromelain from pineapple waste towards acne. Saudi Journal of Biological Sciences 28(1).
Abd Elhamid, MA, Mandour, AS, Ismail, TA, Al-Zohairy, AM, Almowallad S, Alqahtani, LS, Osman A. 2022. Powerful Antioxidants and Cytotoxic Activities of the Methanol Extracts from Eight Soybean Cultivars. Molecules 27(9), 1–12.
Abu Hajleh MN, Khleifat KM, Alqaraleh M, Al-Hraishat E, Al-Limoun MO, Qaralleh H, Al-Dujaili EAS. 2022. Antioxidant and Antihyperglycemic Effects of Ephedra foeminea Aqueous Extract in Streptozotocin-Induced Diabetic Rats. Nutrients 14(11), 1–20.
Ahmed M, Ji M, Sikandar A, Iram A, Qin P, Zhu H. 2022. Extract of Chinese Arrowhead Sagittaria trifolia L .Molecules 24(1), 1-15.
Aini K, Elfita, Widjajanti H, Setiawan A, Kurniawati AR. 2022. Antibacterial activity of endophytic fungi isolated from the stem bark of jambu mawar (Syzygium jambos). Biodiversitas 23(1), 521–532.
Alam B, L? J, G? Q, Khan MA, G?ng J, Mehmood S, Yuán Y, G?ng W. 2021. Endophytic Fungi: From Symbiosis to Secondary Metabolite Communications or Vice Versa? Frontiers in Plant Science 12(12), 1–24.
Astutik S, Pretzsch J, Kimengsi JN. 2019. Asian medicinal plants’ production and utilization potentials: A review. Sustainability 11(19), 1–33.
Benjamin MAZ, Ng SY, Saikim FH, Rusdi NA. 2022. The Effects of Drying Techniques on Phytochemical Contents and Biological Activities on Selected Bamboo Leaves. Molecules 27(19), 1–23.
Bouarab-Chibane L, Forquet V, Lantéri P, Clément Y, Léonard-Akkari L, Oulahal N, Degraeve P, Bordes C. 2019. Antibacterial properties of polyphenols: Characterization and QSAR (Quantitative structure-activity relationship) models. Frontiers in Microbiology 10(4), 1-17.
Caruso DJ, Palombo EA, Moulton SE, Zaferanloo B. 2022. Exploring the Promise of Endophytic Fungi: A Review of Novel Antimicrobial Compounds. Microorganisms 10(10), 1-18.
Collins AE, Saleh TM., & Kalisch, B. E. 2022. Naturally Occurring Antioxidant Therapy in Alzheimer’s Disease. Antioxidants 11(2), 1-17.
El-Gendi H, Saleh AK, Badierah R, Redwan EM, El-Maradny YA, El-Fakharany EM. 2022. A comprehensive insight into fungal enzymes: Structure, classification, and their role in mankind’s challenges. Journal of Fungi 8(1), 1-16.
Elfita, Oktiansyah R, Mardiyanto, Widjajanti H, Setiawan A. 2022. Antibacterial and antioxidant activity of endophytic fungi isolated from Peronema canescens leaves. Biodiversitas 23(9), 4783–4792.
Fadhillah, Elfita, Muharni, Yohandini H, Widjajanti H. 2019. Chemical compound isolated from antioxidant active extract of endophytic fungus Cladosporium tenuissimum in swietenia mahagoni leaf stalks. Biodiversitas 20(9), 2645–2650.
Flieger J, Flieger W, Baj J. 2021. Antioxidants?: Classification, natural sources, activity/capacity. Materials 14(4135), 1–54.
Forman HJ, Zhang H. 2021. Targeting oxidative stress in disease: Promise and limitations of antioxidant therapy. Nature Reviews Drug Discovery 20(9), 689–709.
G?secka M, Siwulski M, Magdziak Z, Budzy?ska S, Stuper-Szablewska K, Niedzielski P, Mleczek M. 2020. The effect of drying temperature on bioactive compounds and antioxidant activity of Leccinum scabrum (Bull.) Gray and Hericium erinaceus (Bull.) Pers. Journal of Food Science and Technology 57(2), 513–525.
Geiger A, Karácsony Z, Geml J, Váczy KZ. 2022. Mycoparasitism capability and growth inhibition activity of Clonostachys rosea isolates against fungal pathogens of grapevine trunk diseases suggest potential for biocontrol. PLoS ONE 17(9), 1–13.
Gutiérrez-Del-río I, López-Ibáñes S, Magadán-Corpas P, Fernández-Calleja L, Pérez-Valero Á, Tuñón-Granda M, Miguélez EM, Villar CJ, Lombó F. 2021. Terpenoids and polyphenols as natural antioxidant agents in food preservation. Antioxidants 10(8), 1-18.
Habisukan UH, Elfita, Widjajanti H, Setiawan A, Kurniawati AR. 2021. Diversity of endophytic fungi in Syzygium aqueum. Biodiversitas 22(3), 1129–1137.
Han P, Zhang X, Xu D, Zhang B, Lai D, Zhou L. 2020. Metabolites from clonostachys fungi and their biological activities. Journal of Fungi 6(4), 1–30.
Hapida Y, Elfita, Widjajanti H, Salni. 2021. Biodiversity and antibacterial activity of endophytic fungi isolated from jambu bol (Syzygium malaccense). Biodiversitas 22(12), 5668–5677.
Hayat J, Akodad M, Moumen A, Baghour M, Skalli A, Ezrari S, Belmalha S. 2020. Phytochemical screening, polyphenols, flavonoids and tannin content, antioxidant activities and FTIR characterization of Marrubium vulgare L. from 2 different localities of Northeast of Morocco. Heliyon 6(11), 1-15.
Ismail A, Wan Ahmad WAN. 2019. Syzygium polyanthum (Wight) Walp: A potential phytomedicine. Pharmacognosy Journal 11(2), 429–438.
Jadid N, Kurniawan E, Himayani CES, Andriyani, Prasetyowati I, Purwani KI, Muslihatin W, Hidayati D, Tjahjaningrum ITD. 2020. An ethnobotanical study of medicinal plants used by the Tengger tribe in Ngadisari village, Indonesia. PLoS ONE 15(7), 1–16.
Ju M, Zhang Q, Wang R, Yan S, Li Z, Li P, Gu P. 2022. Correlation in endophytic fungi community diversity and bioactive compounds of Sophora alopecuroides. Frontiers in Microbiology 13(8), 1–13.
Kapeua-Ndacnou M, de Abreu LM, de Macedo DM, da Nóbrega TF, Pereira CM, Evans HC, Barreto RW. 2023. Assessing the biocontrol potential of clonostachys species isolated as endophytes from coffea species and as mycoparasites of hemileia rusts of coffee in africa. Journal of Fungi 9(2), 1-19.
Kiruba NJM, Saeid A. 2022. An insight into microbial inoculants for bioconversion of waste biomass into sustainable “bio-organic” fertilizers: A bibliometric analysis and systematic literature review. International Journal of Molecular Sciences 23(21), 1-15.
Kumar M, Saurabh V, Tomar M, Hasan M, Changan S, Sasi M, Maheshwari C, Prajapati, U, Singh S, Prajapat RK, Dhumal S, Punia S, Amarowicz R, Mekhemar M. 2021. Mango (Mangifera indica l.) leaves: Nutritional composition, phytochemical profile, and health-promoting bioactivities. Antioxidants 10(2), 1–23.
Kumar N, Goel N. 2019. Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnology Reports 24(1), 1-17.
Kupnik K, Primoži? M, Kokol V, Knez Ž. 2023. Compounds from Avocado (Persea americana L .) Seeds 1(1), 1-17
Liu X min, Liu Y, Shan C hui, Yang X quan, Zhang Q, Xu N, Xu L ying, Song W. 2022. Effects of five extraction methods on total content, composition, and stability of flavonoids in jujube. Food Chemistry: X 14(10), 1-15.
Mascarin GM, da Silva AVR, da Silva TP, Kobori NN, Morandi MAB, Bettiol W. 2022. Clonostachys rosea: Production by Submerged culture and bioactivity against Sclerotinia sclerotiorum and Bemisia tabaci. Frontiers in Microbiology 13(5), 1-17.
Michalak I, Tiwari R, Dhawan M, Alagawany M, Farag MR, Sharun K, Emran TBin, Dhama K. 2022. Antioxidant effects of seaweeds and their active compounds on animal health and production–a review. Veterinary Quarterly 42(1), 48–67.
Miklasi?ska-Majdanik M, K?pa M, Wojtyczka RD, Idzik D, W?sik TJ. 2018. Phenolic compounds diminish antibiotic resistance of Staphylococcus aureus clinical strains. International Journal of Environmental Research and Public Health 15(10), 1-16.
Mohammadi H, Saghaian S. 2022. Factors affecting consumption of different forms of medicinal plants: The case of licorice. Agriculture 12(9), 14-53.
Monagas M, Brendler T, Brinckmann J, Dentali S, Gafner S, Giancaspro G, Johnson H, Kababick J, Ma C, Oketch-Rabah H, Pais P, Sarma N, Marles R. 2022. Understanding plant to extract ratios in botanical extracts. Frontiers in Pharmacology 13(9), 1–10.
Moraes RM, Cerdeira AL, Lourenço MV. 2021. Using micropropagation to develop medicinal plants into crops. Molecules 26(6), 1-17.
Munteanu IG, Apetrei C. 2021. Analytical methods used in determining antioxidant activity: A review. International Journal of Molecular Sciences 22(7), 1-19.
Nordin ML, Othman AA, Kadir AA, Shaari R, Osman AY, Mohamed M. 2019. Antibacterial and cytotoxic activities of the Syzygium polyanthum leaf extract from Malaysia. Veterinary World 12(2), 236–242.
Nortjie E, Basitere M, Moyo D, Nyamukamba P. 2022. Extraction methods, quantitative and qualitative phytochemical screening of medicinal plants for antimicrobial textiles: A review. Plants 11(15), 1-17.
Oktiansyah R, Riyanto R, Tibrani MM, Ulandari, T. 2022. Bay leaves (Syzygium polyanthum) extract as a repellent of Culex quinquefasciatus. Edubiotik 7(02), 197–204.
Ortega HE, Torres-Mendoza D, Caballero EZ, Cubilla-Rios L. 2021. Structurally uncommon secondary metabolites derived from endophytic fungi. Journal of Fungi 7(7), 1-18.
Patra A, Abdullah S, Pradhan RC. 2022. Review on the extraction of bioactive compounds and characterization of fruit industry by-products. Bioresources and Bioprocessing 9(1), 1-19.
Patra AK. 2012. Dietary phytochemicals and microbes. In Dietary Phytochemicals and Microbes 1(9789400739), 1-26.
Piombo E, Guaschino M, Jensen DF, Karlsson M, Dubey M. 2023. Insights into the ecological generalist lifestyle of Clonostachys fungi through analysis of their predicted secretomes. Frontiers in Microbiology, 14(2), 1–16.
Pitt JI, Hocking AD. 2013. Fungi and food spolage. In Journal of Chemical Information and Modeling 53(9), 1-21.
Salmerón-Manzano E, Garrido-Cardenas JA, Manzano-Agugliaro F. 2020. Worldwide research trends on medicinal plants. International Journal of Environmental Research and Public Health 17(10), 1-18.
Sandhu HK, Sinha P, Emanuel N, Kumar N, Sami R, Khojah E, Al-Mushhin AAM. 2021. Effect of ultrasound-assisted pretreatment on extraction efficiency of essential oil and bioactive compounds from citrus waste by-products. Separations 8(12), 1-17.
Serventi L, Flores GA, Cusumano G, Barbaro D, Tirillini B, Venanzoni R, Angelini P, Acquaviva A, Di Simone SC, Orlando G, Zengin G, Menghini L, Ferrante C. 2023. Comparative investigation of antimicrobial and antioxidant effects of the extracts from the inflorescences and leaves of the Cannabis sativa L. cv. strawberry. Antioxidants 12(2), 2-19. Song J, Lei T, Hao X, Yuan H, Sun W, Chen S. 2023. Synergistic Effects of Clonostachys rosea Isolates and Succinate Dehydrogenase Inhibitors Fungicides against Gray Mold on Tomato. Microorganisms 11(1), 1-16.
Suleiman JB, Mohamed M, Abu Bakar AB, Nna VU, Zakaria Z, Othman ZA, Aroyehun AB. 2021. Chemical profile, antioxidant properties and antimicrobial activities of malaysian heterotrigona itama bee bread. Molecules 26(16), 1–14.
Sun ZB, Li SD, Ren Q, Xu JL, Lu X, Sun MH. 2020. Biology and applications of Clonostachys rosea. Journal of Applied Microbiology 129(3), 486–495.
Syarifah, Elfita, Widjajanti H, Setiawan A, Kurniawati AR. 2021. Diversity of endophytic fungi from the root bark of Syzygium zeylanicum and the antibacterial activity of fungal extracts, and secondary metabolite. Biodiversitas 22(10), 4572–4582.
Tamura K, Stecher G, Kumar S. 2021. MEGA11: Molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution 38(7), 1-23.
Vaou N, Stavropoulou E, Voidarou C, Tsigalou C, Bezirtzoglou, E. 2021. Towards advances in medicinal plant antimicrobial activity: A review study on challenges and future perspectives. Microorganisms 9(10), 1–28.
Venkatesan RM, Muthusamy K, Iruthayasamy J, Prithiviraj B, Kumaresan PV, Lakshmanan P, Perianadar IV. 2023. First Report of Clonostachys rosea as a Mycoparasite on Sclerotinia sclerotiorum Causing Head Rot of Cabbage in India. Plants 12(1), 1–18.
Walsh, T. J., Hayden, R. T., & Larone, D. H. 2018. Larone’s Medically Important Fungi. Medically Important Fungi 19(1).
Watanabe, T. (2010). Pictorial Atlas of Soil and Seed Fungi. In Pictorial Atlas of Soil and Seed Fungi.
Xia Y, Liu J, Chen C, Mo X, Tan Q, He Y, Wang Z, Yin J, Zhou G. 2022. The Multifunctions and Future Prospects of Endophytes and Their Metabolites in Plant Disease Management. Microorganisms 10(5), 1–19.
Zehiroglu C, Ozturk Sarikaya SB. 2019. The importance of antioxidants and place in today’s scientific and technological studies. Journal of Food Science and Technology 56(11), 4757–4774.
Zhang QW, Lin LG, Ye WC. 2018. Techniques for extraction and isolation of natural products: A comprehensive review. Chinese Medicine (United Kingdom) 13(1), 1–26.
Zheng R, Li S, Zhang X, Zhao C. 2021. Biological activities of some new secondary metabolites isolated from endophytic fungi: A review study. International Journal of Molecular Sciences 22(2), 1–75.
Zubia CS, Babaran GMO, Duque SMM, Mopera LE, Flandez LEL, Castillo-Israel KAT, Reginio FC. 2023. Impact of drying on the bioactive compounds and antioxidant properties of bignay [Antidesma bunius (L.) Spreng.] pomace. Food Production, Processing and Nutrition 5(1), 1-18.
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