Isolation of endophytic fungi from Hiyung chili peppers of local South Kalimantan (Indonesia) varieties and in vitro tolerance to acidic environment

##plugins.themes.bootstrap3.article.sidebar##

PDF
DOI https://doi.org/10.13057/biodiv/d240723
Issue
Vol. 24 No. 7 (2023)
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

##plugins.themes.bootstrap3.article.main##

WITIYASTI IMANINGSIH ♥
Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Lambung Mangkurat. Jl. A. Yani Km 36.4, Banjarbaru 70714, South Kalimantan, Indonesia
NURAENI EKOWATI
Faculty of Biology, Universitas Jenderal Soedirman. Jl. dr. Suparno No. 63, Purwokerto Utara, Banyumas 53122, Central Java, Indonesia
https://orcid.org/0000-0002-5927-0967
SALAMIAH
Department of Plant Protection, Faculty of Agriculture, Universitas Lambung Mangkurat. Jl. A. Yani Km 36.4, Banjarbaru 70714, South Kalimantan, Indonesia
https://orcid.org/0000-0003-4171-1231
NUNIEK INA RATNANINGTYAS
Faculty of Biology, Universitas Jenderal Soedirman. Jl. dr. Suparno No. 63, Purwokerto Utara, Banyumas 53122, Central Java, Indonesia
https://orcid.org/0000-0002-7841-1999
LOEKAS SOESANTO
Faculty of Agriculture, Universitas Jenderal Soedirman. Jl. dr. Suparno No. 61 Grendeng, Purwokerto Utara, Banyumas 53122, Central Java, Indonesia
https://orcid.org/0000-0002-6501-4177

Abstract

Abstract. Imaningsih W, Ekowati N, Salamiah, Ratnaningtyas NI, Soesanto L. 2023. Isolation of endophytic fungi from Hiyung chili peppers of local South Kalimantan (Indonesia) varieties and in vitro tolerance to acidic environment. Biodiversitas 24: 3844-3852. Hiyung chili (Capsicum frutescens L) is a local chili from Hyung Village, Tapin District, South Kalimantan Province, Indonesia which has the advantage of being cultivated with an agricultural system in swampland, with high production and is known to have high capsaicin also. This advantage is thought to be related to its symbiosis with endophytic microbes, especially fungi. The purpose of this study was to isolate and identify non-pathogenic endophytic fungi from Hiyung chili plants and determine their ability to tolerate acidic conditions in vitro. Results showed that a total of 41 endophytic isolates were isolated from different parts of chili plant. Of the 41 isolates four non-pathogenic endophytic fungi were isolated from leaf tissue. Based on morphological and molecular identification these four fungi were Pseudozyma hubeiensis DM3.1, Phoma sp. DT2.2P1, Letendraea sp. DM1.2 and Phoma sp. DT2.2P4. Four endophytic fungi showed different abilities in an in vitro preliminary study of acidic environmental stress tolerance. Only P. hubeiensis DM3.1 isolate had the daily diameter of colony not significantly different based on the Anova test (? :0.05, F: 1.092, P:0.372) both at pH 5, 4 and 3 and without pH treatment.

##plugins.themes.bootstrap3.article.details##

References
REFERENCES
Abhijeet Singh, Y.M., 2014. Understanding the Biodiversity and Biological Applications of Endophytic Fungi: A Review. J. Microb. Biochem. Technol. s8. https://doi.org/10.4172/1948-5948.S8-004
Aguilera, A., González-Toril, E., 2019. Eukaryotic Life in Extreme Environments: Acidophilic Fungi. Fungi Extrem. Environ. Ecol. Role Biotechnol. Significance 21–38. https://doi.org/10.1007/978-3-030-19030-9_2
Aveskamp, M.M., De Gruyter, J., Crous, P.W., 2008. Biology and recent developments in the systematics of Phoma, a complex genus of major quarantine significance. Fungal Divers. 31, 1–18.
Barnett, J.., Payne, R.W., Yarrow, D., 2000. Yeast?: Characteristics and identification. Third Edition.
Black, W.D., 2020. A comparison of several media types and basic techniques used to assess outdoor airborne fungi in Melbourne, Australia. PLoS One 15, 1–25. https://doi.org/10.1371/journal.pone.0238901
Budi, I.S., Mariana, M., 2016. Controlling Anthracnose Disease of Locally Chili in Marginal Wetland using Endophytic Indigenous Microbes and Kalakai (Stenochlaena palustris) Leaf Extract. J. Wetl. Environ. Manag. 4, 28–34. https://doi.org/10.20527/jwem.v4i1.51
de Silva, N.I., Maharachchikumbura, S.S.N., Thambugala, K.M., Bhat, D.J., Karunarathna, S.C., Tennakoon, D.S., Phookamsak, R., Jayawardena, R.S., Lumyong, S., Hyde, K.D., 2021. Morpho-molecular taxonomic studies reveal a high number of endophytic fungi from Magnolia candolli and M. garrettii in China and Thailand. Mycosphere 12, 163–237. https://doi.org/10.5943/mycosphere/12/1/3
Direktorat Merek dan Indikasi Geografis KEMENKUMHAM, 2019. Berita Resmi Indikasi Geografis Seri-A no.No. 17/IG/X/A/2019. Direktorat Merek dan Indikasi Geografis. Direktorat Jenderal Kekayaan Intelektual. KEMENKUMHAMRI.
Fajarningsih, N.D., 2016. Internal Transcribed Spacer (ITS) as Dna Barcoding to Identify Fungal Species: a Review. Squalen Bull. Mar. Fish. Postharvest Biotechnol. 11, 37. https://doi.org/10.15578/squalen.v11i2.213
Francis, F., Fingu-Mabola, J.C., Ben Fekih, I., 2022. Direct and Endophytic Effects of Fungal Entomopathogens for Sustainable Aphid Control: A Review. Agric. 12. https://doi.org/10.3390/agriculture12122081
Gai, C.S., Lacava, P.T., Maccheroni, W., Glienke, C., Araújo, W.L., Miller, T.A., Azevedo, J.L., 2009. Diversity of endophytic yeasts from sweet orange and their localization by scanning electron microscopy. J. Basic Microbiol. 49, 441–451. https://doi.org/10.1002/jobm.200800328
Gao, H., Wang, Yanan, Luo, Q., Yang, L., He, X., Wu, J., Kachanuban, K., Wilaipun, P., Zhu, W., Wang, Yi, 2021. Bioactive Metabolites From Acid-Tolerant Fungi in a Thai Mangrove Sediment. Front. Microbiol. 11, 1–14. https://doi.org/10.3389/fmicb.2020.609952
Giauque, H., Connor, E.W., Hawkes, C. V., 2019. Endophyte traits relevant to stress tolerance, resource use and habitat of origin predict effects on host plants. New Phytol. 221, 2239–2249. https://doi.org/10.1111/nph.15504
Hamdani, Salawati, U., Nuryadin, R., 2017. Daya Saing Agribisnis Dan Potensi Pengembangan Cabe Hiyung Di Kabupaten Tapin, Kalimantan Selatan. Pros. Semin. Nas. Lahan Basah Jilid 1, 156–163.
Hibbett, D.S., Binder, M., Bischoff, J.F., Blackwell, M., Cannon, P.F., Eriksson, O.E., Huhndorf, S., James, T., Kirk, P.M., Lücking, R., Thorsten Lumbsch, H., Lutzoni, F., Matheny, P.B., McLaughlin, D.J., Powell, M.J., Redhead, S., Schoch, C.L., Spatafora, J.W., Stalpers, J.A., Vilgalys, R., Aime, M.C., Aptroot, A., Bauer, R., Begerow, D., Benny, G.L., Castlebury, L.A., Crous, P.W., Dai, Y.C., Gams, W., Geiser, D.M., Griffith, G.W., Gueidan, C., Hawksworth, D.L., Hestmark, G., Hosaka, K., Humber, R.A., Hyde, K.D., Ironside, J.E., Kõljalg, U., Kurtzman, C.P., Larsson, K.H., Lichtwardt, R., Longcore, J., Miadlikowska, J., Miller, A., Moncalvo, J.M., Mozley-Standridge, S., Oberwinkler, F., Parmasto, E., Reeb, V., Rogers, J.D., Roux, C., Ryvarden, L., Sampaio, J.P., Schüßler, A., Sugiyama, J., Thorn, R.G., Tibell, L., Untereiner, W.A., Walker, C., Wang, Z., Weir, A., Weiss, M., White, M.M., Winka, K., Yao, Y.J., Zhang, N., 2007. A higher-level phylogenetic classification of the Fungi. Mycol. Res. 111, 509–547. https://doi.org/10.1016/j.mycres.2007.03.004
Imaningsih, W., Mariana, Junaedi, A.B., Rasyidah, 2021. Antifungal activities of the combination of ulin wood liquid smoke and hiyung cayenne pepper root endophyte fungi against Colletothricum capsici. Agrivita 43, 69–78. https://doi.org/10.17503/agrivita.v1i1.2458
Joubert, P.M., Doty, S.L., 2018. Endophytic Yeasts: Biology, Ecology and Applications 3–14. https://doi.org/10.1007/978-3-319-89833-9_1
K., R.P., Barnett, H.L., Hunter, B.B., 1972. Illustrated Genera of Imperfect Fungi. Mycologia 64, 930. https://doi.org/10.2307/3757954
Lee, G., Lee, S.H., Kim, K.M., Ryu, C.M., 2017. Foliar application of the leaf-colonizing yeast Pseudozyma churashimaensis elicits systemic defense of pepper against bacterial and viral pathogens. Sci. Rep. 7, 1–13. https://doi.org/10.1038/srep39432
Palma, M., Guerreiro, J.F., Sá-Correia, I., 2018. Adaptive response and tolerance to acetic acid in Saccharomyces cerevisiae and Zygosaccharomyces bailii: A physiological genomics perspective. Front. Microbiol. 9, 1–16. https://doi.org/10.3389/fmicb.2018.00274
Pramudyani, L., Sabran, M., Noor, A., 2019. Agronomic Performance and Nutrition Content of Hiyung as Local Variety of Cayenne Pepper [Capsicum frutescens] at Dry Land and Swamp Land of South Kalimantan Province. Bul. Plasma Nutfah 25, 43. https://doi.org/10.21082/blpn.v25n1.2019.p43-52
Rajamanikyam, M., Vadlapudi, V., Amanchy, R., Upadhyayula, S.M., 2017. Endophytic Fungi as Novel Resources of natural Therapeutics. Arch. Biol. Technol. v. Arch. Biol. Technol. v 606060, 1–26. https://doi.org/10.1590/1678-4324-2017160542
Rigobelo, E.C., Baron, N.C., 2021. Endophytic fungi: a tool for plant growth promotion and sustainable agriculture. Mycology 00, 1–17. https://doi.org/10.1080/21501203.2021.1945699
Rosana, Y., Matsuzawa, T., Gonoi, T., Karuniawati, A., 2014. Modified Slide Culture Method for Faster and Easier Identification of Dermatophytes. Microbiol. Indones. 8, 135–139. https://doi.org/10.5454/mi.8.3.7
Rosas-Moreno, J., Walker, C., Duffy, K., Krüger, C., Krüger, M., Robinson, C.H., Pittman, J.K., 2023. Isolation and identification of arbuscular mycorrhizal fungi from an abandoned uranium mine and their role in soil-to-plant transfer of radionuclides and metals. Sci. Total Environ. 876, 162781. https://doi.org/10.1016/j.scitotenv.2023.162781
Rousk, J., Brookes, P.C., Ba?a?th, E., 2009. Contrasting Soil pH Effects on Fungal and Bacterial Growth Suggest Functional Redundancy in Carbon Mineralization. Appl. Environ. Microbiol. 75, 1589–1596. https://doi.org/10.1128/AEM.02775-08
Sarma, P., Dkhar, M.S., Kayang, H., Kumar, M., Dubey, N.K., Raghuwanshi, R., 2020. Diversity of endophytic fungi associated with hedychium spicatum ham ex sm. And their antifungal activity against the phytopathogen Alternaria solani. Stud. Fungi 5, 84–93. https://doi.org/10.5943/sif/5/1/8
Soesanto, L., Hartono, A.R.R., Mugiastuti, E., Widarta, H., 2020. Seed-borne pathogenic fungi on some soybean varieties. Biodiversitas 21, 4010–4015. https://doi.org/10.13057/biodiv/d210911
Soltani Nejad, M., Samandari Najafabadi, N., Aghighi, S., Pakina, E., Zargar, M., 2022. Evaluation of Phoma sp. Biomass as an Endophytic Fungus for Synthesis of Extracellular Gold Nanoparticles with Antibacterial and Antifungal Properties. Molecules 27. https://doi.org/10.3390/molecules27041181
Stone, J.K., Polishook, J.D., JR., J.F.W., 2012. Endophytic Fungi, in: Endophytic Fungi. Wiley, pp. 1–6. https://doi.org/10.1002/9780470015902.a0027206
Sutomo, Rahmawati, A., Rizki, M.I., 2017. Standarisasi Buah Cabe Rawit Hiyung ( Capsicum frutescens L .) Asal Tapin Kalimantan Selatan 2, 245–253. https://doi.org/https://doi.org/10.36387/jiis.v2i2.121
Terna, T.P., Nor, N.M.I.M., Zakaria, L., 2022. Histopathology of Corn Plants Infected by Endophytic Fungi. Biology (Basel). 11. https://doi.org/10.3390/biology11050641
Vylkova, S., 2017. Environmental pH modulation by pathogenic fungi as a strategy to conquer the host. PLOS Pathog. 13, e1006149. https://doi.org/10.1371/journal.ppat.1006149
Wahdah, R., Rahmawati, Y., Nafarin, Y., Sunarwo, Taufikurrahman, Masyraniansyah, Wagimin, B.M, S., Budiono, A., Yamani, Bardiah, Raudah, Normakiah, L.P., 2016. Deskripsi Cabai Rawit Varietas Hiyung. LAMPIRAN SURAT KEPUTUSAN MENTERI PERTANIAN REPUBLIK INDONESIA NOMOR?: 031/Kpts/SR.120/D.2.7/4/2016 DESKRIPSI.
Wang, L.W., Xu, B.G., Wang, J.Y., Su, Z.Z., Lin, F.C., Zhang, C.L., Kubicek, C.P., 2012. Bioactive metabolites from Phoma species, an endophytic fungus from the Chinese medicinal plant Arisaema erubescens. Appl. Microbiol. Biotechnol. 93, 1231–1239. https://doi.org/10.1007/s00253-011-3472-3
Watanabe, T., 2003. Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species. Choice Rev. Online 40, 40-3398-40–3398. https://doi.org/10.5860/CHOICE.40-3398
Xu, Y., Fu, N., Ding, W., Wang, P., Xu, J., 2021. Three new polyketides from the insect-associated fungus letendraea sp. 5xnz4-2. Rec. Nat. Prod. 15, 187–193. https://doi.org/10.25135/rnp.206.20.08.1786