Morphological study and DNA fingerprint pattern of Goniothalamus tapis and G. umbrosus (Annonaceae) using M13 marker and its taxonomic implication

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DOI https://doi.org/10.13057/biodiv/d241152
Issue
Vol. 24 No. 11 (2023)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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IMAM MAHADI ♥
Biology Education Program, Faculty of Teacher Training and Education, Universitas Riau. Jl. H.R. Subrantas Km 12.5, Pekanbaru 28293, Riau, Indonesia
F. FATHURRAHMAN
Department of Agrotechnology, Faculty of Agriculture, Universitas Islam Riau. Jl. Kaharuddin Nst. No. 113, Pekanbaru 28284, Riau, Indonesia
NERY SOFIYANTI
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Riau. Jl. Subrantas Km. 12,5, Pekanbaru 28293, Riau, Indonesia
AGUS SUSATYA
Department of Forestry, Faculty of Agriculture, Universitas Bengkulu. Jl. WR Supratman, Bengkulu 21170, Bengkulu, Indonesia

Abstract

Abstract. Mahadi I, Fathurrahman F, Sofiyanti N, Susatya A. 2023. Morphological study and DNA fingerprint pattern of Goniothalamus tapis and G. umbrosus (Annonaceae) using M13 marker and its taxonomic implication. Biodiversitas 24: 6299-6306. The species of Goniothalamus (Blume) JD Hooker & Thomson (Annonaceae) is widely considered a source of traditional medicine and containsoniothalamin, which is used for anti-cancer and anti-bacterial drugs. Goniothalamus tapis and G. umbrosus are traditional medicines in Sumatra, Indonesia, and Malaysia Peninsula, respectively. The community uses this plant as a source of traditional medicinal ingredients. Clinically, Goniothalamus contains the compound goniothalamin, an anti-cancer and anti-bacterial drug. The existence of this plant is gradually maintained. G. tapis is often used as a traditional medicine for the Malay community in Sumatra, and G. umbrosus is widely used by the Malay community in the Peninsular Malaysia. These two plants are morphologically similar, but the two species are different. Some researchers consider these plants to be the same. For this reason, it needs to be studied by combining two methods, both molecular and morphological. G. tapis and G. umbrosus are species of the Goniothalamus distributed in Sumatra and Peninsular Malaysia. Their taxonomic status needs to be clarified due to the morphological character similarity. This study aimed to study the morphological characters of G. tapis and G. umbrosus as well as their DNA fingerprint pattern using M13 Marker. The morphological examination showed that G. tapis and G. umbrosus characters are distinguished by leaf, flower, and fruit. This result is supported by a DNA fingerprint pattern using an M13 marker. The results show that these two species are different both in terms of morphology and fingerprints. So, it can be concluded that these two species are different.

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References
Aslam, M.S., Ahmad, MS., Mamat, AS., Ahmad, MZ., Salam, F. 2016. Goniothalamus: phytochemical and ethnobotanical review. Recent Advances in Biology and Medicine. 2: 34-47. DOI: 10.18639/RABM.2016.02.292264.
Ay?e Gül ?nce. Mehmet Karaca. 2019. Td DAMD PCR Markers for Myrtle (Myrtus communis L.) Genetic Studies. Agriculture and Forestry Research. 2(1). 530-536.
Carneiro Lúcio, ASS, Da Silva Almeida, JRG, Leitãoda-Cunha, EV, Fechine Tavares, J & Filho, JM. 2015. Alkaloids of the Annonaceae: occurrence and a compilation of their biological activities. The Alkaloids: Chemistry and Biology. 74:233-409. DOI:10.1016/bs.alkal.2014. 09.002.
Charan Leeratiwong, Piya Chalermglin, Richard M. K. Saunders. 2021. Goniothalamus roseipetalus and G. sukhirinensis (Annonaceae): Two new species from Peninsular Thailand. PhytoKeys 184: 1–17. DOI: 10.3897/phytokeys.184.73210.
Chin Cheung Tang, Daniel C. Thomas, Richard, MK. Saunders. 2015. Molecular phylogenetics of the species-rich angiosperm genus Goniothalamus (Annonaceae) inferred from nine chloroplast DNA regions: Synapomorphies and putative correlated evolutionary changes in fruit and seed morphology. Molecular Phylogenetics and Evolution. 92: 124-139.https://doi.org/10.1016/j. ympev.2015.06.016.
De Souza, CAS, Nardelli, VB & Paz, WHP. 2020. Asarone-derived phenylpropanoids and isoquinoline-derived alkaloids from the bark of Duguetia pycnastera (Annonaceae) and their cytotoxicities. Química Nova. 43(10):1397-1403. DOI:10.21577/0100-4042. 20170617
Doyle, J.J & Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.
Dylan G. 2022. Peopling island rainforests: global trends from the Early Pleistocene to the Late Holocene. World Archaeolog. 54 (2): 338-362, DOI: 10.1080/00438243.2022.2121316.
Guo, X, Tang ,CC, Thomas, DC, Couvreur, TLP, Saunders, RMK. 2017. A mega-phylogeny of the Annonaceae: Taxonomic placement of five enigmatic genera and recognition of a new tribe, Phoenicantheae. Scientific Reports 7(1): e7323. DOI: 10.1038/s41598-017-07252-2
Ibrahimi M, Brhadda N, Ziri R, Fokar M, Iraqi D, Gaboun F, Labhilili M, Habach A, Meziani R, Elfadile J, Abdelwahd R, Diria G. 2023. Analysis of genetic diversity and population structure of Moroccan date palm (Phoenix dactylifera L.) using SSR and DAMD molecular markers. J Genet Eng Biotechnol. 21(1): 66. doi: 10.1186/s43141-023-00516-7.
Ilham Adhya, Pudji Widodo, Cecep Kusmana, Eming Sudiana, Imam Widhiono, Toto Supartono. 2020. Population structure and habitat characteristics of Goniothalamus macrophyllus in Bukit Pembarisan forest, West Java, Indonesia. BIODIVERSITAS. 21 (3): 1130-1135. Doi : 10.13057 /biodiv/d210337.
Kartonegoro Abdulrokhman, Mota de Oliveira Sylvia, Welzen, PC. 2021. Historical biogeography of the Southeast Asian and Malesian tribe Dissochaeteae (Melastomataceae). Journal of Systematics and Evolution. 60 (2): 237-252. doi.org/10.1111/jse.12752.
Kim Rosalind PK. Liew, Sook Yee Liew, Khalijah Awang. 2020. Phytochemical constituents from the stem barks of Goniothalamus tapis Miq. Biochemical Systematics and Ecology. 93. 104185. Doi: 10.1016/j.bse.2020.104185.
Leeratiwong C, Chalermglin P, Saunders RMK. 2021. Goniothalamus roseipetalus and G.sukhirinensis (Annonaceae): Two new species from Peninsular Thailand. Phytokeys. 25 (184):1-17. Doi: 10.3897/Phytokeys.184.73210.
Li, LK, Rola, AS, Kaid, FA, Ali, AM & Alabsi, AM. 2016. Goniothalamine induces cell cycle arrest and apoptosis in H400 human oral squamous cell carcinoma: a caspase-dependent mitochondrial-mediated pathway with downregulation of NF-kappabeta. Arch Oral Biol, 2016; 64:28–38.
Linh Thuy, T.T., Nhi Yen, T. D., Nguyen Thao, N. L., Hoai, T. N., Duc,V.H. , Thao, T. D., Manh Hung, T., Tan Khanh, N & Phu Tran, V.P. 2022. In Silico and in Vitro Evaluation of Alkaloids from Goniothalamus elegans Ast. for Breast Cancer Treatment. Natural Product Communications. 17(3): 1–10. DOI: 10.1177/1934578X221088110
Mahadi, I.,Wulandari, S., Safii, W & Sayuti, I. 2021. Suspension Cell Culture Of Gajah Beranak (Goniothalamus Tapis Miq) On Goniothalamine Compound Content. Jurnal Agro. 8 (2): 247-261. . https://doi.org/10.15575/14710 (Indonesian).
Mat-Salleh, K. 2001. New and noteworthy species of Bornean Goniothalamus (Annonacea). Folia malaysiana.Vol.2(2): 75-116.
Metcalfe, I. 2013. Tectonic Evolution of the Malay Peninsula. Journal of Asian Earth Sciences. 76. 195-213. 10.1016/j.jseaes.2012.12.011.
Metcalfe, I. 2017. Tectonic evolution of Sundaland. Bulletin of the Geological Society of Malaysia. 63: 27-60. Doi: 10.7186/bgsm63201702.
Natasa Mohd Shakri, Wan Mohd Nuzul Hakimi Wan Salleh, Shazlyn Milleana Shaharudin. 2021. Review on Malaysian Goniothalamus essential oils and their comparative study using multivariate statistical analysis. Nat. Volatiles & Essent. Oils. 8(1): 1-12. DOI: 10.37929/nveo.786172.
Nordin, N, Majid, N.A, Hashim, N.M, Rahman, M.A, Hassan, Z & Ali, H. M. 2015. Liriodenine, an aporphine alkaloid from Enicosanthellum pulchrum, inhibits proliferation of human ovarian cancer cells through induction of apoptosis via the mitochondrial signaling pathway and blocking cell cycle progression. Drug Des Devel Ther; 9:1437. DOI:10.2147/DDDT.S77727
Patompong, K, Wilart, P & Ratana, B. 2019. Goniothalamin Induces Necroptosis and Anoikis in Human Invasive Breast Cancer MDA-MB-231 Cells. Int. J. Mol. Sci. 2019, 20, 3953; DOI:10.3390/ijms20163953
Saunders, RMK. 2003. A synopsis of Goniothalamus species (Annonaceae) in Peninsular Malaysia, with a description of a new species. Bot.J. 142: 321-339.
Saunders, RMK 2002. The genus Goniothalamus (Annonaceae) in Sumatra. Botanical Journal of the Linnean Society. 139: 225 - 254. 10.1046/j.1095-8339.2002.00061.x.
Selma, Ben Abdelaali. Olfa Saddoud Debbabi, Nasr Ben Abdelaali. 2017. Fingerprinting Of On-Farm Conserved Local Tunisian Orange Cultivars (Citrus Sinensis (L.) Osbeck) Using Microsatellite Markers. PAN. 60(1): 83–93
Selvakumari E, Jenifer J, Priyadharshini S, Vinodhini R. 2017. Application of DNA Fingerprinting for Plant Identification. Journal of Academia and Industrial Research. 5 (10):149-151.
Seyed, MA, Jantan I, Bukhari SN. 2014. Emerging anticancer potentials of goniothalamin and its molecular mechanisms. Biomed Res Int. 2014;2014:536508. DOI: 10.1155/2014/536508.
Sinclair, J. 1955. A revision of the Malayan Annonaceae. Gardens’ Bulletin, Singapore 14: 423-446.
Thomas DC, Tang CC, Saunders RMK (2017) Historical biogeography of Goniothalamus and Annonaceae tribe Annoneae: Dispersal-vicariance patterns in tropical Asia and inter¬continental tropical disjunctions revisited. Journal of Biogeography 44(12): 2862–2876. DOI: 10.1111/jbi.13086.
Suchaichit, N, Kanokmedhakul K, Panthama N, Poopasit K, Moosophon P, Kanokmedhakul S. A. 2015. 2H-tetrahydropyran derivative and bioactive constituents from the bark of Goniothalamus elegants Ast. Fitoterapia. 103:206-212. DOI:10.1016/J.FITOTE. 2015.04.005
Syaza, S.F., Noor Zarina, A. W., Leong, C. Y & Nazlina, I. Styrylpyrone Derivative from Goniothalamus sp.: A Powerful Drug for Fighting Against Herpes Simplex Virus Type 1. 2021. Pharmacogn J. ; 13(6): 1598-1606. DOI : 10.5530/pj.2021.13.206
Yang B, Zhu RB, Ding HB, Bouamanivong S, Tan YH. 2020. A new species and two new records of Goniothalamus (Annonaceae) from Lao PDR. PhytoKeys. 10 (138): 17-25. doi: 10.3897/phytokeys.138.38995.
Zheng Y, Xu S, Liu J, Zhao Y, Liu J. 2017. Genetic diversity and population structure of Chinese natural bermudagrass [Cynodon dactylon (L.) Pers.] germplasm based on SRAP markers. PLoS One. 12(5): e0177508. doi: 10.1371/journal.pone.0177508.