Novel exploration of the presence and morphological features Spirometra (Cestoda: Diphyllobothriidae) in Javan spitting cobra snakes (Naja sputatrix) in Sidoarjo, Indonesia
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Abstract. Edila R, Effendi MH, Suwanti LT, Kwon H-K, Agumah NB. 2024. Novel exploration of the presence and morphological features Spirometra (Cestoda: Diphyllobothriidae) in Javan spitting cobra snakes (Naja sputatrix) in Sidoarjo, Indonesia. Biodiversitas 25: 3318-3324. The study aimed to investigate the presence of spargana infection and provide a detailed description of the morphology, morphometry and histology in Javan spitting cobra snakes (Naja sputatrix Boie 1827) from Sidoarjo, Indonesia. Ethical approval was obtained and 51 living wild-caught N. sputatrix were collected from local sellers. All samples were euthanized and observed for the presence of plerocercoid. Identification of the plerocercoid as the larval infective stage was made using a carmine staining method drawn by using a camera lucida and routine histological study using hematoxylin-eosin staining. The positive rate of snakes in this study was 54.9%. A total of 165 spargana were collected which divided 82(49.6%) infecting muscles, 31 (16.7%) in viscera and 52 (31.5%) located in subcutaneous tissues. The plerocercoid length and width were 50-95 mm and 1.42-1.81 mm. Morphological analysis confirmed Spirometra characteristics and histological sections provided insights into spargana's structural complexity. This study represents the first detailed exploration of the morphological features, morphometry, and histology of Spirometra plerocercoids in Indonesia. The high prevalence of spargana in N. sputatrix underscores the importance of improving epidemiological databases, stringent wildlife trade regulations, and awareness programs to prevent sparganosis in Indonesia.
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References
Ambu S, Kavana NJ, Lim LHS. 2021. The morphological characteristics of the sparganum stage of the Malaysian Spirometra species. Intl E-J Sci Med Edu 9 (2): 22. DOI: 10.56026/imu.9.2.22.
Bar?ák D, Yoneva A, Sehadová H, Oros M, Gustinelli A, Kuchta R. 2019. Complex insight on microanatomy of larval “human broad tapeworm” Dibothriocephalus latus (Cestoda: Diphyllobothriidea). Parasit Vectors 12 (1): 1-17. DOI: 10.1186/s13071-019-3664-8.
Edila R, Effendi MH, Suwanti LT, KWON HK, Yudhana A, Wardhana, AH, Alhada A. 2024. A comprehensive study on the occurrence rate and morphology characteristics of the acanthocephalan parasite in Javan spitting cobra (Naja sputatrix) in Sidoarjo, Indonesia. Biodiversitas 25 (2): 516-521. DOI: 10.13057/biodiv/d250210.
Edila R, Yudhana A, Praja RN. 2023. First report of Physaloptera (Nematoda: Physalopteridae) parasitizing Javan spitting cobra snake (Naja sputatrix) in Indonesia. AIP Conf Proceed 243: 1. DOI: 10.1063/5.0115940.
Hong Q, Feng J, Liu H, Li X, Gong L, Yang Z, Yang W, Liang X, Zheng R, Cui Z, Wang W, Chen D. 2016. Prevalence of Spirometra mansoni in dogs, cats, and frogs and its medical relevance in Guangzhou, China. Intl J Infect Dis 53: 41-45. DOI: 10.1016/j.ijid.2016.10.013.
Jeon HK, Park H, Lee D, Choe S, Kim KH, Huh S, et al. 2015. Human infections with Spirometra decipiens plerocercoids identified by morphologic and genetic analyses in Korea. Kor J Parasitol 53 (3): 299. DOI: 10.3347/kjp.2015.53.3.299.
Jongthawin J, Intapan PM, Sanpool O, Sadaow L, Laymanivong S, Thanchomnang T, Maleewong W. 2014. Molecular evidence of Spirometra erinaceieuropaei infection in snakes Ptyas korros from Lao PDR and Thailand and frogs Hoplobatrachus rugulosus from Myanmar. Southeast Asian J Trop MedPublic Health 45 (6): 1271.
Kavana NJ, Lim LHS, Ambu S. 2014. The life-cycle of Spirometra species from Peninsular Malaysia. Trop Biomed 31: 487-495.
Kikuchi T, Maruyama H. 2020.Human proliferative sparganosis update. Parasitol Intl 75: 102036. DOI: 10.1016/j.parint.2019.102036.
Kim BM, Kim DJ, Chang MY, Kim YJ, Kim JH, You JK. 2020. Axillary sparganosis, changes in ultrasound images over six months: A case report. Radiol Case Rep 15 (3): 177-180. DOI: 10.1016/j.radcr.2019.11.008.
Kim JG, Ahn CS, Sohn WM, Nawa Y, Kong Y. 2018. Human sparganosis in Korea. J Kor Med Sci 33 (44): 1-15. DOI: 10.3346/jkms.2018.33.e273.
Kudo T, Fujioka A, Korenaga M, Yamasaki H, Morishima Y, Sugiyama H. 2017. Molecular identification of intramuscular and subcutaneous Spirometra erinaceiuropaei sparganosis in a Japanese patient. J Dermatol 44 (6): e138-9. DOI: 10.1111/1346-8138.13739.
Laovachirasuwan P, Mootsikapun P, Maleewong W, Intapan PM. 2015. Intraosseous proliferative sparganosis presenting as a pathologic fracture: A case report and review of the literature. Southeast Asian J Trop Med Public Health 46 (4): 558-63.
Liu DZ, Tian H, Song ZM. 2007. Report on a trade survey of the snakes in some Chinese key cities. Proceedings of the Snake Resource Protecting Seminar 12-33.
Liu Q, Li MW, Wang ZD, Zhao GH, Zhu XQ. 2015. Human sparganosis, a neglected food borne zoonosis. Lancet Infect Dis 10:1226-1235. DOI: 10.1016/S1473-3099(15)00133-4.
Morales A, Laird-Pérez RM, Capó V, Iglesias E, Fonte L, Plascencia-Hernández A. 2022. Genetic and morphological identification of Spirometra decipiens in snakes and domestic dog found in Cuba. Pathogens 11 (12): 1468. DOI: 10.3390/pathogens11121468.
Omar HM, Fahmy M, Abuowarda M. 2023. Hand palm sparganosis: Morphologically and genetically confirmed Spirometra erinaceieuropaei in a fourteen-year-old girl. J Egypt Soc Parasitol 47 (4): 859-864. DOI: 10.1007/s12639-023-01623-5.
Ooi HK, Chang SL, Huang CC, Kawakami Y, Uchida. 2000. A survey of Spirometra erinaceieuropaei in frogs in Taiwan and its experimental infection in cats. J Helminthol 74 (2): 173-176. DOI: 10.1017/S0022149X0000024X.
Plumeriastuti H, Pranashinta GT, Suwanti LT, Proboningrat A. 2023. First report of pentastomiasis and the lung histopathological changes in Oriental Rat Snake (Ptyas mucosa) in Sidoarjo, East Java, Indonesia. Biodiversitas 24 (4): 2045-2051. DOI: 10.13057/biodiv/d240414.
Pranashinta GT, Suwanti LT, Koesdarto S, Poetranto ED. 2017. Spirometra in Ptyas mucosus snake in Sidoarjo, Indonesia. KnE Life Sci 34-40. DOI: 10.18502/kls.v3i6.1104.
Vargas-Parada L, Laclette JP. 1999. Role of calcareous corpuscles in cestode physiology. Rev Latinoam Microbiol 41: 303-307.
Wang F, Zhou L, Gong S, Deng Y, Zou J, Wu J. 2011. Severe infection of wild-caught snakes with Spirometra erinaceieuropaei from food markets in Guangzhou, China involves a risk for zoonotic sparganosis. J Parasitol 97 (1): 170-171. DOI: 10.1645/GE-2519.1.
Wu ZJ, Chen Y, Qiu XL, Jiang HT. 2007. An investigation of plerocercoid infection of frogs in Guiyang city and an analysis on clinical characteristics of 104 cases. J Guiyang Med Coll 32 (1): 140-141.
Xiao L, Ryan U, Feng Y. 2015. Diphyllobothrium, Diplogonoporus and Spirometra. In: Kuchta R, Scholz T, Brabec J, Narduzzi-Wicht B (eds). Biology of Foodborne Parasites Section III Important Foodborne Parasites. CRC Press, Boca Raton.
Yudhana A, Novita PR, Koesoemo WD, Nurwartanti YM. 2020a. Public health relevance of sparganosis in Javan spitting cobra snakes (Naja sputatrix): A neglected zoonotic disease in Indonesia. Indian J Public Health Res Dev 11 (3): 4-8.
Yudhana A, Praja RN, Kartikasari AM. 2021. Sparganosis (Spirometra spp.) in Asian water monitor (Varanus salvator): A medical implications for veterinarians, breeders, and consumers. Vet World 14 (9): 2482-2487. DOI: 10.14202/vetworld.2021.2482-2487.
Yudhana A, Praja RN, Wardhana DK, Yunita MN, Fikri F, Fransiska EM. 2020b. Public health relevance of sparganosis in Javan spitting cobra snakes (Naja sputatrix): A neglected zoonotic disease in Indonesia. Indian J Public Health Res Dev 11 (3): p2258. DOI: 10.37506/ijphrd.v11i3.2713.
Yudhana A, Praja RN, Yunita MN, Wardhana DK, Fikri F. 2020c. Prevalence of Spirometra in white-lipped green pit viper (Trimeresurus insularis) in Banyuwangi City, Indonesia. J Vet Parasitol 34 (1): 12-16. DOI: 10.5958/0974-0813.2020.00003.0.
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