Analysis of heat-shock protein genes and their expression in Lactiplantibacillus plantarum SU-KC1a




Abstract. Julyanto CMP, Dosan R, Victor H, Sugata M, Tan TJ. 2024. Analysis of heat?shock protein genes and their expression in Lactiplantibacillus plantarum SU-KC1a. Biodiversitas 25: 2494-2499. Lactiplantibacillus plantarum is lactic acid bacteria commonly used as probiotics. Throughout the manufacturing and processing stages for incorporation into functional food products, probiotics encounter different stress conditions, including heat stress, which can affect their viability. In this study, the ability of L. plantarum SU-KC1a to withstand heat stress conditions was assessed by measuring cell viability after incubation at 37, 42, 47, and 52? for one hour. Additionally, the growth of SU-KC1a in liquid cultures under various high temperatures was monitored hourly for three hours. Total RNA was extracted from each culture, converted into cDNA, and amplified using hsp3 gene-specific primers. The results indicated a slower growth rate of SU-KC1a at higher temperatures, with 42? as the maximum temperature supporting growth. Visualization of hsp3 amplicon showed increased intensity following incubation at higher temperatures, indicating upregulation of hsp3 gene expression. To identify the presence of heat stress-related genes, whole genome sequence annotation data of SU-KC1a was compared to that of L. plantarum SK151, F75, and WCFS1. Lactiplantibacillus plantarum was found to possess two systems involved in protein repair in response to heat stress damage: chaperone and protease. In summary, L. plantarum SU-KC1a harbors heat shock protein genes, and their expression is upregulated following exposure to high temperatures.


Adamberg K, Adamberg S, Laht TM, Paalme T. 2003. The effect of temperature and pH on the growth of lactic acid bacteria: A pH-auxostat study. Int J Food Microbiol 85(1-2): 171-83. DOI:
Angelis MD, Cagno RD, HUE C, Crecchio C, Fox PF, Gobbetti M. 2004. Heat shock response in Lactobacillus plantarum. Appl Environ Microbiol 70(3): 1336-1346.DOI:
Arena MP, Capozzi V, Longo A, Russo P, Weidmann S, Rieu A, Guzzo J, Spano G, Fiocco D. 2019. The phenotypic analysis of Lactobacillus plantarum sHSP mutants reveals a potential role for hsp1 in cryotolerance. Front Microbiol 10: 838. DOI:
Butel MJ. 2014. Probiotics, gut microbiota and health. Méd Maladies Infectieuses 44(1): 1-8. DOI:
Capozzi V, Weidmann S, Fiocco D, Rieu A, Hols P, Guzzo J, Spano G. 2011. Inactivation of a small heat shock protein affects cell morphology and membrane fluidity in Lactobacillus plantarum WCFS1. Res Microbiol 162: 419-425. DOI:
Chang Z. 2016. The function of the DegP (HtrA) protein: Protease versus chaperone. IUBMB Life 68(11): 904-907. DOI:
Flores M, Hsiao T, Chiu Y, Chuang EY, Huang F, CHEN Y. 2013. Gene regulation, modulation, and their applications in gene expression data analysis. Adv Bioinform 360678. DOI:
Krewing M, Cremers CM, Schubert B, Mullerm A, Leichert LIO, Bandow J, Stephanek JJ, Lackman JM, Awakowicz, P, Jacob U. 2019. The molecular chaperone Hsp33 is activated by atmospheric-pressure plasma protecting proteins from aggregation. J Royal Soc Interface 16(155): 20180966. DOI:
Liao Q, Hang X, Liu X, Pan J, Zhang H, Yang H. 2010. The influence of pH on heat stress response by probiotic Lactobacillus plantarum LP-Onlly. Annals Microbiol 60: 341-348. DOI:
Lin, TH, Huang SC, Haw GC. 2012. Reexamining transcriptional regulation of the Bacillus subtilis htpX Gene and the ykrK Gene, encoding a novel type of transcriptional regulator, and redefining the YkrK operator. J Bacteriol 194(24): 6758-6765. DOI:
Liu R, Kim AH, Kwak MK, Kang SO. 2017. Proline-based cyclic dipeptides from Korean fermented vegetable kimchi and from Leuconostoc mesenteroides LBP-K06 have activities against multidrug-resistant bacteria. Front Microbiol 8: 761. DOI:
Longo A, Russo P, Capozzi V, Spano G, Fiocco D. 2021. Knock out of sHSP genes determines some modifications in the probiotic attitude of Lactiplantibacillus plantarum. Biotechnol Lett 43(3): 645-654. DOI:
Ma J, Xu C, Liu F, Hou J, Shao H, Yu W. 2021. Stress adaptation and cross-protection of Lactobacillus plantarum KLDS 1.0628. J Food 19(1): 72-80. DOI:
Matejcekova Z, Liptakova D, Spodniakova S, Valik L. 2016. Characterization of the growth of Lactobacillus plantarum in milk in dependence on temperature. Acta Chimica Slovaca 9(2): 104-108. DOI: 10.1515/acs-2016-0018
Membre JM, Leporq B, Mettler E, Thuault D, Zwietering M, Vialette M, Perrier L. 2005. Temperature effect on bacterial growth rate: Quantitative microbiology approach including cardinal values and variability estimates to perform growth simulations on/in food. Int J Food Microbiol 100(1-3): 179-86. DOI:
Mitchell K, Iadarola MJ. 2010. RT-PCR analysis of pain genes: Use of gel-based RT-PCR for studying induced and tissue-enriched gene expression. Methods in Mol Biol 617: 279-295. DOI:
Noor R. 2015. Mechanism to control the cell lysis and the cell survival strategy in stationary phase under heat stress. SpringerPlus 4: 599. DOI:
Papadimitriou K, Alegria A, Bron PA, Angelis MD, Gobbetti M, Kleerebezem M, Lemos JA, Linares DM, Ross P, Stanton C, Turroni F, Sinderen, DV, Varmanen P, Ventura M, Zuniga M, Tsakalidou E, Kok J. 2016. Stress physiology of lactic acid bacteria. Microbiol and Mol Biol Rev 80(3): 873-890. DOI:
Seddik HA, Bendali F, Gancel F, Fliss, I, Spano G, Drider D. 2017. Lactobacillus plantarum and its probiotic and food potentialities. Probiotics Antimicrob Protein 9: 111-122. DOI:
Van Bokhorst-Van de Veen H, Bongers RS, Wels M, Bron PA, Kleerebezem M. 2013. Transcriptome signatures of class I and III stress response deregulation in Lactobacillus plantarum reveal pleiotropic adaptation. Microb Cell Fact 12(1): 112. DOI:
Webster JM, Darling AL, Uversky VN, Blair LJ. 2019. Small heat shock proteins, big impact on protein aggregation in neurodegenerative disease. Front Pharmacol 10(1047): 1-18. DOI:
WHO. 2001. Probiotics in Food: Health and Nutritional Properties and Guidelines for Evaluation. World Health Organization: Food and Agriculture Organization of the United Nations, Rome.
Yu AO, Goldman EA, Brooks JT, Golomb BL, Yim IS, Gotcheva V, Angelov A, Kim EB, Marco ML. 2021. Strain diversity of plant associated Lactiplantibacillus plantarum. Microb Biotechnol 14(5): 1990-2008. DOI: