Analysis of two strain probiotics on digestive enzymes, liver function and antimicrobial activity of catfish (Clarias gariepinus) treated with Aeromonas hydrophila
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Aini N, Wahyuningsih SPA, Uzakky FN, Betzy C, Fatimah, Andriyono S. 2024. Analysis of two strain probiotics on digestive enzymes, liver function and antimicrobial activity of catfish (Clarias gariepinus) treated with Aeromonas hydrophila. Biodiversitas 25: 4333-4339. Catfish (Clarias gariepinus Burchell 1822) is one of the most consumed fish. Good fisheries management is needed to maintain fish stocks. One of the efforts to manage fisheries safely is by using probiotics in the form of Duo Strain Probiotics (DSP). DSP is a probiotic formula consisting of Lactobacillus casei and Bacillus subtilis. The purpose of this study was to determine the effect of DSP on digestive enzyme levels, liver health status, and the potential of catfish blood serum as an antimicrobial agent. The treatments included DSP to the feed with concentrations of 0%, 5%, 10% and 15% using Aeromonas hydrophila infection. The results showed no significant change in amylase enzyme level between DSP treatments of various concentrations and the control treatment. DSP treatment of 10% and 5% in the non-infected fish group was the best treatment for increasing the level of protease and lipase enzymes, respectively. For liver health status parameters, 15% DSP treatment was the best treatment that could suppress the production of AST and ALT enzymes in fish blood serum. Catfish blood serum infected with pathogens and treated with 15% DSP has the ability as an antibacterial agent against A. hydrophila.
##plugins.themes.bootstrap3.article.details##
References
Abdul-Malik A, Aliyu-Paiko M, Adamu KM, Aliyu AA, Mohammed JN. 2023. Role of prebiotic, probiotic and symbiotic diets on bacterial proliferation in feed and intestine of African catfish (Clarias gariepinus). J Appl Sci Environ Manag 27: 87-93. DOI: 10.4314/jasem.v27i1.13.
Adorian TJ, Jamali H, Farsani HG, Darvishi P, Hasanpour S, Bagheri T, Roozbehfar R. 2019. Effects of probiotic bacteria Bacillus on growth performance, digestive enzyme activity, and hematological parameters of Asian sea bass, Lates calcarifer (Bloch). Probiotics Antimicrob Proteins 11: 248-255. DOI: 10.1007/s12602-018-9393-z.
Aini N, Bachruddin M, Fatimah F, Andriyono S, Wahyuningsih SPA. 2024a. Effect of Lactobacillus casei FNCC 0090 to improve gastrointestinal bacterial abundance, immune system and water quality in catfish farming. Biodiversitas 25 (5): 2130-2138. DOI: 10.13057/biodiv/d250531.
Aini N, Bachruddin M, Tsana I. 2020. Microbial isolation from gastrointestinal tract of catfish (Clarias gariepinus). J Microbial Syst Biotechnol 2 (1): 22-30. DOI: 10.37604/jmsb.v2i1.38.
Aini N, Hariani D. 2019. Effect of pineapple peel filtrate and probiotic Lactobacillus casei FNCC 0090 on catfish growth (Clarias gariepinus). AIP Conference Proceedings. The 9th International Conference on Global Resource Conservation (ICGRC) and Aji from Ritsumeikan University, 7-8 March 2018, Malang, Indonesia. DOI: 10.1063/1.5061910.
Aini N, Putri DSYR, Achhlam DH, Fatimah F, Andriyono S, Hariani D, Do HDK, Wahyuningsih SPA. 2024b Supplementation of Bacillus subtilis and Lactobacillus casei to increase growth performance and immune system of catfish (Clarias gariepinus) due to Aeromonas hydrophila infection. Vet World 17 (3): 602-611. DOI: 10.14202/vetworld.2024.602-611.
Amenyogbe E, Droepenu EK, Ayisi CL, Boamah GA, Duker RQ, Abarike ED, Huang J. 2024. Impact of probiotics, prebiotics, and synbiotics on digestive enzymes, oxidative stress, and antioxidant defense in fish farming: Current insights and future perspectives. Front Mar Sci-Sec Aquat Physiol 11: 1368436. DOI: 10.3389/fmars.2024.1368436.
Apriliyanto T, Anindita NS, Susila WA. 2021. Antibacterial activity of isolates from digestive tract of catfish (Clarias gariepinus) as probiotic candidates at PT. Biotech Cipta Kreasi. Intl J Health Sci Technol 3 (1): 138-149. DOI: 10.31101/ijhst.v3i1.2240.
Arani MM, Salati AP, Safari O, Keyvanshokooh S. 2019. Dietary supplementation effects of Pediococcus acidilactiti as probiotic on growth performance, digestive enzyme activities, and immunity response in zebrafish (Danio rerio). Aquac Nutr 25 (4): 854-861. DOI: 10.1111/anu.12904.
Assan D, Kuebutornye FKA, Hlordzi V, Chen H, Mraz J, Mustapha UF, Abarike ED. 2022. Effects of probiotics on digestive enzymes of fish (finfish and shellfish); status and prospects: A mini review. Comp Biochem Physiol Part B. 257: 110653. DOI: 10.1016/j.cbpb.2021.110653.
Baisakhi B, Swain HS, Bera AK. 2024. Bacillus subtilis and Saccharomyces cerevisiae as potential modulators of hemato-biochemical indices, digestive enzymes and disease resistance in Labeo rohita. Agric Res 2024: 1-10. DOI: 10.1007/s40003-024-00795-7.
Barrett KE, Brooks H, Boitano S, Barman SM. 2010. Ganong’s review of medical physiology. 23rd ed. McGraw-Hill Medical, New York.
Cavalcante RB, Telli GS, Tachibana L, Dias DC, Oshiro E, Natori MM, da Silva WF, Jose M, Ranzani PMJ. 2020. Probiotics, prebiotics and synbiotics for nile tilapia: Growth performance and protection against Aeromonas hydrophila infection. Aquac Rep 17: 100343. DOI: 10.1016/j.aqrep.2020.100343.
Das BK, Nidhi RGN, Roy P, Muduli AK, Swain P, Mishra SS, Jayasankar P. 2014. Antagonistic activity of cellular components of Bacillus subtilis AN11 against bacterial pathogens. Int J Curr Microbiol App Sci 3 (5): 795-809.
Dasgupta A. 2015 Liver enzymes as alcohol biomarkers. In: Alcohol and its biomarkers: Clinical aspects and laboratory determination. Elsevier Inc, USA. DOI: 10.1016/B978-0-12-800339-8.00005-5.
Dawood MAO. 2021. Nutritional immunity of fish intestines: Important insights for sustainable aquaculture. Rev Aquac 13: 642-663. DOI: 10.1111/raq.12492
Diwan AD, Harke SN, Panche AN. 2023. Host-microbiome interaction in fish and shellfish: An overview. Fish Shellfish Immunol Rep 4: 100091. DOI: 10.1016/j.fsirep.2023.100091.
Docan A, Dediu L, Cristea V. 2011. Effect of feeding with different dietary protein level on hematological indices of juvenile Siberian sturgeon, Acipenser baeri reared under recirculating systems condition. AACL Bioflux 4 (2):180-186.
Ehsannia S, Ahari H, Kakoolaki S, Anvar SA, Yousefi S. 2022. Effects of probiotics on zebrafish model infected with Aeromonas hydophila Spatial distribution, antimicrobial, and hispatological investigation. BMC Microbiol 22: 167. DOI: 10.1186/s12866-022-02491-4.
Ene OC, Ogbu P, Joshi RK. 2020. Phytochemical antimicrobial and proximate analysis of the leaves of Mirabilis jalapa from Uvwie Delta state, Nigeria. Intl J Herb Med 8: 39-45.
Febrianti R, Khasani I, Rosada KK. 2021. Assessing the susceptibility of the selected gourami (Osphronemus goramy) to Aeromonas hydrophila. Nusantara Biosci 13: 111-120. DOI: 10.13057/nusbiosci/n130115.
Giri SK, Sukumaran V, Oviya M. 2013. Potential probiotic Lactobacillus plantarum VSG3 improves the growth, immunity, and disease resistance of tropical freshwater fish, Labeo rohita. Fish Shellfish Immunol 34 (2): 660-666. DOI: 10.1016/j.fsi.2012.12.008.
Gobi N, Vaseeharan B, Chen JC, Rekha R, Vijayakumar S, Anjugam M, Iswarya A. 2018. Dietary supplementation of probiotic Bacillus licheniformis Dahb1 improves growth performance, mucus and serum immune parameters, antioxidant enzyme activity as well as resistance against Aeromonas hydrophila in tilapia Oreochromis mossambicus. Fish Shellfish Immunol 74: 501-508. DOI: 10.1016/j.fsi.2017.12.066.
Hai NV. 2015. Research findings from the use of probiotics in tilapia aquaculture: A review. Fish Shellfish Immunol 45 (2): 592-597. DOI: 10.1016/j. fsi.2015.05.026.
Hamid N, Hassan M, Sabri M, Nadia A, Hamdan R, Afifah MN, Raina M, Nadia AB, Fuad M. 2016. Studies of pathogenicity effect of Aeromonas hydophila infection in juvenile red hybrid tilapia Oreochromis sp. Proceed Int Seminar on Livestock Prod Vet Technol 2016: 532-539. DOI: 10.14334/Proc.Intsem.LPVT-2016-p.532-539.
Hastuti S, Subandiyono S, Windarto S. 2019 Blood performance of jaundice catfish Clarias gariepinus. AACL Bioflux 12 (2): 480-489.
Hastuti S, Subandiyono S. 2020. Aminotransferase, hematological indices and growth of tilapia (Oreochomis niloticus) reared in various stocking densities in aquaponic systems. AACL Bioflux 13: 813-824.
Kari ZA, Wee W, Sukri SAM, Harun HC, Reduan MFH, Khoo MI, van Doan H, Goh KW, Wei LS. 2022. Role of phytobiotics in relieving the impacts of Aeromonas hydrophila infection on aquatic animals: A mini-review. Front Vet Sci 9: 1023784. DOI: 10.3389/fvets.2022.1023784.
Kong YD, Kang YH, Tian JX, Zhang DX, Zhang L, Tao LT, Wu TL, Li Y, Wang Q, Shan XF. 2019. Oral immunization with recombinant Lactobacillus casei expressing flaB confers protection against Aeromonas veronii challenge in common carp, Cyprinus carpio. Fish Shellfish Immunol 87: 627-637. DOI: 10.1016/j.fsi.2019.01.032.
Kusuma PSW, Hariani D, Mukti AT. 2022. Evaluation of probiotic-fermented feed addition and laser-firing to accelerate mature brood stocks and seed productions of African catfish (Clarias gariepinus). Turk J Fish Aquac Sci 22 (2): 19303. DOI: 10.4194/TRJFAS19303.
Lawal MO, Lawal AZ, Adewumi GA, Mudiaga A. 2019. Growth, nutrient utilization, haematology and biochemical parameters of african catfish (Clarias gariepinus, Burchell, 1822) fed with varying levels of Bacillus subtilis. Agrosearch 19: 13-27. DOI: 10.4314/agrosh.v19i1.2.
Luo N, Wang L, Wang Z, Xiao B, Wang N, Yu X, Wu D, Song Z. 2022. Effects of dietary supplementation of duo-strain probiotics with post-spraying technology on growth performance, digestive enzyme, antioxidant capacity and intestinal microbiota of grass carp (Ctenopharyngodon idella). Aquac Rep 26: 101301. DOI: 10.1016/j.aqrep.2022.101301
Moghaddam BS, Pourjaafari M. 2021. The effects of four types of specific probiotic on growth performance, liver enzymes and immune indices of juvenile Persian sturgeon (Acipenser persicus). Iran J Fish Sci 20 (4): 1179-1191. DOI: 10.22092/ijfs.2021.350966.0.
Mollanourozi A, Khalili HB, Kazempoor R, Alavinezhad S. 2021. Probiotic supplements as an alternative medicine; investigation the effect of Lactobacillus casei on liver function of koi fish (Cyprinus rubrofuscus L.) in exposure to pathogen as an animal model. J Surv Fish 8 (1): 119-126. DOI: 10.18331/SFS2021.8.1.9.
Monier MN, Kabary H, Elfeky A, Saadony S, El-Hamed E, Nadia NBA, Eissa E, Moaheda EH, and Eissa EH. 2023. The effects of Bacillus species probiotics (Bacillus subtilis and B. licheniformis) on the water quality, immune responses, and resistance of whiteleg shrimp (Litopenaeus vannamei) against Fusarium solani infection. Aquac Intl 31: 3437-3455. DOI: 10.1007/s10499-023-01136-1.
Mulia DS, Apriyanti W, Maryanto H, Purbomartono C. 2012. Immunogenicity antigen bacteria whole cell Aeromonas hydrophila). Sains Akuatik 14 (1): 25-32.
Putra AN, Syamsunarno MB, Ningrum W, Jumyanah M. 2020. Effect of the administration of probiotic Bacillus NP5 in the rearing media on water quality, growth, and disease resistance of African catfish (Clarias gariepinus). Biodiversitas 21: 2566-2575. DOI: 10.13057/biodiv/d210629.
Qiu M, Wang Y, Wang X, Sun L, Ye R, Xu D, Dai Z, Liu Y, Bi S, Yao Y, Gooneratne R. 2016. Effects of T-2 toxin on growth, immune function and hepatopancreas microstructure of shrimp (Litopenaeus vannamei). Aquac 462: 35-39. DOI: 10.1016/j.aquaculture.2016.04.032.
Safari R, Imanpour MR, Hoseinifar SH, Faheem M, Dadar M, van Doan H. 2022. Effects of dietary Lactobacillus casei on the immune, growth, antioxidant, and reproductive performances in male zebrafish (Danio rerio). Aquac Rep 25: 101176. DOI: 10.1016/j.aqrep.2022.101176.
Semwal A, Kumar A, Kumar N. 2023. A review on pathogenicity of Aeromonas hydrophila and their mitigation through medicinal herbs in aquaculture. Heliyon 9: e14088. DOI: 10.1016/j.heliyon.2023.e14088.
Sudrajat RH, Yuhana M, Widanarni W, Julie E, Afiff U. 2023. Dietary supplementation of Bacillus sp. NP5 and dayak onion simplicia powder Eleutherine bulbosa (Mill.) Urb. for the prevention of Aeromonas hydrophila in catfish Clarias sp. Jurnal Akuakultur Indonesia 22 (2): 134-146. DOI: 10.19027/jai.22.2.134-146.
Sumon TA, Hussain MA, Sumon MAA, Jang WJ, Abellan FG, Sharifuzzaman SM, Brown CL, Lee E-W, Kim C-H, Hasan MT. 2022. Functionality and prophylactic role of probiotics in shellfish aquaculture. Aquac Rep 25: 101220. DOI: 10.1016/j.aqrep.2022.101220.
Trukhachev VI, Chmykhalo VK, Belanova AA, Beseda DK, Chikindas ML, Bren AB, Ermakov AM, Donnik IM, Belousova MM, Zolotukhin PV. 2021. Probiotic biomarkers and models upside down: From humans to animals. Vet Microbiol 261: 109156. DOI: 10.1016/j.vetmic.2021.109156.
Vieco SN, Belguesmia Y, Raspoet R, Auclair E, Gancel F, Kempf I, Drider D. 2019. Benefits and inputs from lactic acid bacteria and their bacteriocins as alternatives to antibiotic growth promoters during food-animal production. Front Microbiol 10: 57. DOI: 10.3389/fmicb.2019.00057.
Wang J, Zhu Z, Li R, Wang X, Leng X, Chen, L. 2021. Impact of supplementary Lactobacillus casei K17 on growth and gut health of largemouth bass Micropterus salmoides. Aquac Rep 20: 100734. DOI: 10.1016/j.aqrep.2021.100734.
Wang YQ, Wang K, Xing PJ, Wang J. 2020. Dietary cinnamaldehyde and Bacillus subtilis improve growth performance, digestive enzyme activity, and antioxidant capability and shape intestinal microbiota in tongue sole Cynoglossus semilaevis. Aquac 543: 735798. DOI: 10.1016/j.aquaculture.2020.735798.
Wilkins T and Sequoia J. 2017. Probiotics for gastrointestinal conditions: A summary of the evidence. Am Fam Physician 96 (3): 170-178.
Zhang DX, Kang YH, Chen L, Siddiqui SA, Wang CF, Qian AD, Shan XF. 2018. Oral immunization with recombinant Lactobacillus casei expressing OmpAI confers protection against Aeromonas veronii challenge in common carp, Cyprinus carpio. Fish Shellfish Immunol 72: 552-563. DOI: 10.1016/j.fsi.2017.10.043.
Zhu C, Yu L, Liu W, Jiang M, He S, Yi G, Wen H, Liang X. 2019. Dietary supplementation with Bacillus subtilis LT3-3 enhances the growth, immunity and disease resistance againts Streptococcus agalactiae infection in genetically improved farmed tilapia Oreochromis niloticus. Aquac Nutr 25 (6): 1241-1249. DOI: 10.1111/anu.12938.
Most read articles by the same author(s)
- FARAH AISYAH NAFIDIASTRI, RIZKY DANANG SUSETYO, TRI NURHARIYATI, AGUS SUPRIYANTO, ALMANDO GERALDI, NI’MATUZAHROH, FATIMAH, SALAMUN, Biosurfactant activity of indigenous Bacillus sp. ES4.3 isolated from endemic breeding sites of dengue hemorrhagic fever vector in Surabaya, East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 12 (2021)
- APON ZAENAL MUSTOPA, INES FERDIANA PUSPITASARI, FATIMAH FATIMAH, LITA TRIRATNA, GINA KARTINA, Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR , Biodiversitas Journal of Biological Diversity: Vol. 19 No. 5 (2018)
- FATIMAH, RAHAYU, JAMAL WIWOHO, SUNNY UMMUL FIRDAUS, PUJIYONO, MARIMIN, DWI PRIYO ARIANTO, AHMAD PRAMONO, Genetic diversity of eucalypts for germplasm conservation in Forest Area with the Special Purpose of Mount Bromo, Karanganyar, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 10 (2021)
- FATIMAH, FATICHATUS SUROIYAH, NIKMATUS SOLIKHA, NAZIL DWI RAHAYUNINGTYAS, TINI SURTININGSIH, TRI NURHARIYATI, NI’MATUZAHROH, MOCH. AFFANDI, ALMANDO GERALDI, AHMAD THONTOWI, Antimicrobial activity of actinomycetes isolated from mangrove soil in Tuban, East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 6 (2022)
- AGMAL QODRI, ENCILIA, YULIZAH, DEDEN GIRMANSYAH, SUNARDI, WAHYUDI SANTOSO, MEGAWATI, RINA RACHMATIYAH, FATIMAH, DARMAWAN, SARINO, DJUNIJANTI PEGGIE, Recorded and predicted butterflies in the Padang Bindu Karst, South Sumatra, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 2 (2023)
- FATIMAH, LILLAH ASRITAFRIHA, SYARIFAH SALSABILA, ERNA KRISTIANA DEWI, NI’MATUZAHROH, ALMANDO GERALDI, RICO RAMADHAN, HERY SUWITO, ARRIZAL RAHMAN, LUKMAN RIYADI, Potential of antimicrobial-producing endophytic bacteria from Balikpapan endemic ginger (Etlingera balikpapanensis A.D. Poulsen) , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 9 (2024)
- SINAR PAGI SEKTIANA, ANNUR AHADI ABDILLAH, MD. JOBAIDUL ALAM, WAHYU ISRONI, NINA NURMALIA DEWI, HYUN WOO KIM, SAPTO ANDRIYONO, Diversity of the Tabuhan Island coral reef fish revealed by DNA barcoding and implication on conservation strategy in Banyuwangi, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 9 (2022)
- SALAMUN, RIZKY DANANG SUSETYO, NI’MATUZAHROH, FATIMAH, ALMANDO GERALDI, AGUS SUPRIYANTO, TRI NURHARIYATI, FARAH AISYAH NAFIDIASTRI, NABILATUN NISA’, ENDARTO, Biosurfactant production of entomopathogenic Bacillus subtilis BK7.1, as potential biocontrol bacteria, isolated from Baluran National Park, East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 3 (2023)
- BAYU PRANATA, ARADEA BUJANA KUSUMA, VERA SABARIAH, HYUN WOO KIM, SAPTO ANDRIYONO, Environmental DNA metabarcoding reveals biodiversity marine fish diversity of a small island at Manokwari District, West Papua, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 11 (2022)