Diversity of Ceratocystis fimbriata causing canker and wilt disease on Cupressus sempervirens (Italian cypress) in Indonesia
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Pratama R, Suwandi S, Muslim A, Mulawarman. 2025. Diversity of Ceratocystis fimbriata causing canker and wilt disease on Cupressus sempervirens (Italian cypress) in Indonesia. Biodiversitas 26: 278-287. In 2022-2023, new diseases were observed on Cupressus sempervirens in South Sumatra, Indonesia, with the disease incidence increasing from 17.6% to 26.5% in 2023. Initial symptoms, included stem cankers, black lesions on sapwood and vascular tissue, discoloration and partial wilting of leaves, and eventual complete drying, leading to plant death. The objective of this study was to isolate and identify the fungal pathogen causing wilt disease in C. sempervirens trees using morphological characterization and DNA sequencing. In 2022-2023, a disease survey was conducted in six districts of South Sumatra. The results showed that six out of ten locations were infected, with disease incidence ranging from 4.1% to 17.6% in 2022, increasing to 2% to 26.5% in 2023. Pathogen identification employed a polyphasic approach, combining morphological and molecular characteristics from specific genomic regions (the internal transcribed spacer (ITS) and ?-tubulin). Both the morphological features (including a globose base with a long neck-ended tip with ostiolar hyphae, cylindrical conidia, and hat-shaped ascospores) and phylogenetic analysis identified the isolates as Ceratocystis fimbriata. ITS gene sequences indicated that all the isolates belonged to the ITS5 haplotype. In pathogenicity test, pathogen caused mortality in C. sempervirens, Acacia mangium, and Artocarpus heterophyllus plants. The implications of these findings are significant, as they can potentially lead to the development of effective control measures. To our knowledge, this is the first report of Ceratocystis spp. causing wilt disease on C. sempervirens in South Sumatra.
##plugins.themes.bootstrap3.article.details##
References
Al-Adawi AO, Barnes I, Khan IA, Al Subhi AM, Al Jahwari AA, Deadman ML, Wingfield BD, Wingfield MJ. 2013. Ceratocystis manginecans is associated with a serious wilt disease of two native legume trees in Oman and Pakistan. Aust Plant Pathol 42: 179-193. DOI: 10.1007/s13313-012-0196-5.
Alam MW, Rehman A, Iqbal M, Saira M, Aslam S, Muhammad S, Hameed A, Gleason ML. 2017. First report of Ceratocystis fimbriata causing Eucalyptus Wilt in Pakistan. Dis Notes 101 (6): 1050-1050. DOI: 10.1094/PDIS-12-16-1703-PDN.
Amelia RP, Muslim A, Damiri N, Hamidson H, Suwandi, Pratama R. 2024. Ceratocystis fimbriata on brown salwood (Acacia mangium) in Banyuasin, Indonesia. J Agric Sci 8: 104-110. DOI: 10.25081/jsa.2024.v8.9367.
Batiha GES, Teibo JO, Shaheen HM, Akinfe OA, Awad AA, Teibo TKA, Alexiou A, Papadakis M. 2023. Bioactive compounds, pharmacological actions and pharmacokinetics of Cupressus sempervirens. Naunyn-Schmiedeberg's Arch Pharmacol 396 (3): 389-403. DOI: 10.1007/s00210-022-02326-z.
Brito RAS, Cavalcante G, Borel F, Maffia LA. 2019. Detection and isolation of Ceratocystis fimbriata in mango trees on semi-selective medium. Eur J Plant Pathol 155: 667-669. DOI: 10.1007/s10658-019-01785-0.
Chi NM, Thu PQ, Hinh TX, Dell B. 2019. Management of Ceratocystis manginecans in plantations of Acacia through optimal pruning and site selection. Australas Plant Pathol 48 (4): 343-350. DOI: 10.1007/s13313-019-00635-1.
d’Auria A, Vingiani S, Marzaioli F, Teobaldelli M, Pasquale GD. 2020. Recent history, use and forgetfulness of the Cypress forest of Fontegreca (Southern Italy). J Diver 12 (12): 461. DOI: 10.3390/d12120461.
Danti R, Rocca GD. 2017. Epidemiological history of Cypress canker disease in source and invasion sites. Forests 8 (4): 121. DOI: 10.3390/f8040121.
Fourie A, Wingfield MJ, Wingfield BD, Barnes I. 2015. Molecular markers delimit cryptic species in Ceratocystis sensu stricto. Mycol Prog 14: 1020. DOI: 10.1007/s11557-014-1020-0.
Galovi?ová L, ?miková N, Schwarzová M, Vukic MD, Vukovic NL, Kowalczewski P?, Bakay L, Kluz MI, Puchalski C, Obradovic AD, Mati? MM, Ka?ániová M. 2023. Biological activity of Cupressus sempervirens essential oil. Plants 12 (5): 1097. DOI: 10.3390/plants12051097.
Harrington TC, Huang Q, Ferreira MA, Alfenas AC. 2015. Genetic analyses trace the Yunnan, China population of Ceratocystis fimbriata on pomegranate and taro to populations on Eucalyptus in Brazil. Plant Dis 99 (1): 106-111. DOI: 10.1094/pdis-01-14-0056-re.
Harrington TC, Kazmi MR, Al-Sadi AM, Ismail SI. 2014. Intraspecific and intragenomic variability of ITS rDNA sequences reveals taxonomic problems in Ceratocystis fimbriata sensu stricto. Mycologia 106: 224-242. DOI: 10.3852/13-189.
Khan MF, Ahamad T, Rawat P. 2017. Biomedicinal and chemical profile of Cupressus sempervirens: A mini review. Insights Biomed 2 (3): 16. DOI: 10.21767/2572-5610.10032.
Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33 (7): 1870-1874. DOI: 10.1093/molbev/msw054.
Li J, Zhang Y, Xu KC, Yang JY, Han YH, Sun YX, Huang Q. 2014. First report of wilt of Eucalyptus caused by Ceratocystis fimbriata in China. Plant Dis 98 (12): 1744. DOI: 10.1094/pdis-06-14-0580-pdn.
Li Q, Harrington TC, McNew D, Li J, Huang Q, Somasekhara YM, Alfenas AC. 2016. Genetic bottlenecks for two populations of Ceratocystis fimbriata on sweet potato and pomegranate in China. Plant Dis 100 (11): 2266-2274. DOI: 10.1094/pdis-03-16-0409-re.
Liu F, Mbenoun M, Barnes I, Roux J, Wingfield MJ, Li G, Li J, Chen S. 2015. New Ceratocystis species from Eucalyptus and Cunninghamia in South China. Antonie Van Leeuwenhoek 107 (6): 1451-173. DOI: 10.1007/s10482-015-0441-3.
Mahdikhani M, Matinfar M, Aghaalikhani A. 2017. First report of Phytophthora austrocedri causing phloem lesions and bronzing on Cupressus sempervirens in northern Iran. New Dis Rep 36 (10): 2044-0588. DOI: 10.5197/j.2044-0588.2017.036.010.
Milenkovi? I, Radulovi? Z, Karadži? D. 2022. First report of Seiridium cardinale on Cupressus sempervirens in Serbia. Plant Prot Sci 58 (4): 360-364. DOI: 10.17221/54/2021-PPS.
Muslim A, Hyakumachi M, Kageyama K, Suwandi S, Pratama R. 2019. A rapid bioassay to evaluate efficacy of Hypovirulent Binucleate Rhizoctoniain reducing Fusarium Crown and root rot of tomato. The Open Agric J13: 27-33. DOI: 10.2174/1874331501913010027.
Muslim A, Pratama R, Suwandi S, Hamidsoon H. 2022. Diseases severity, genetic variation, and pathogenicity of Ceratocystis wilt on Lansium domesticum in South Sumatra, Indonesia. Plant Pathol 38 (2): 131-145. DOI: 10.5423/ppj.oa.12.2021.0182.
Muslim A, Suwandi S, Pratama R, Gunawan B. 2025. Ceratocystis fimbriata causing canker and wilt disease on West Indian mahogany trees in Indonesia. J Plant Dis Prot 132 (12): 1-14. DOI: 10.1007/s41348-024-00995-x.
Nasution A, Glen M, Beadle C, Mohammed C. 2019. Ceratocystis wilt and canker: A disease that compromises the growing of commercial Acacia-based plantations in the tropics. Aust For 82: 80-93. DOI: 10.1080/00049158.2019.1595347.
Nouri AB, Dhifi W, Bellili S, Ghazghazi H, Aouadhi C, Chérif A, Hammami M, Mnif W. 2015. Chemical composition, antioxidant potential, and antibacterial activity of essential oil cones of Tunisian Cupressus sempervirens. J Chem 2015 (5): 538929. DOI: 10.1155/2015/538929.
Oliveira LSS, Harrington TC, Ferreira MA, Damacena MB, Al-Sadi AM, Al-Mahmooli HIS, Alfenas AC. 2015. Species or genotypes? Reassessment of four recently described species of the Ceratocystis wilt pathogen, Ceratocystis fimbriata, on Mangifera indica. Phytopathol 105 (9): 1229-1244. DOI: 10.1094/phyto-03-15-0065-r.
Oliveira LSS, Harrington TC, Ferreira MA, Freitas RG, Alfenas AC. 2017. Populations of Ceratocystis fimbriata on Colocasia esculenta and other hosts in the Mata Atlantica region in Brazil. Plant Pathol 67 (1): 97-106. DOI: 10.1111/ppa.12706.
Orhan IE, Tumen I. 2015. Potential of Cupressus sempervirens (Mediterranean Cypress) in Health. 1st ed. In: Preedy VR, Watson RR (eds). The Mediterranean Diet: An Evidence-Based Approach. Academic Press, Cambridge. DOI: 10.1016/B978-0-12-407849-9.00057-9.
Pansera MR, Silvestre WP, Sartori VC. 2023. Bioactivity of Cupressus sempervirens and Cupressus lusitanica leaf essential oils on Colletotrichum fructicola. J Essent Oil Res 35 (1): 51-59. DOI: 10.1080/10412905.2022.2104393.
Piveta G, Ferreira MA, Muniz MFB, Valdetaro D, Valdebenito-Sanhuezad R, Harrington T, Alfenas AC. 2016. Ceratocystis fimbriata on kiwifruit (Actinidia spp.) in Brazil. N. Z. J Crop Hortic Sci 44 (1): 13-24. DOI: 10.1080/01140671.2016.1143020.
Pratama R, Muslim A, Suwandi S, Damiri N, Soleha S. 2021a. First report of bullet wood (Mimusops elengi) sudden decline disease caused by Ceratocystis manginecans in Indonesia. Biodiversitas 22 (5): 2636-2645. DOI: 10.13057/biodiv/d220522.
Pratama R, Muslim A, Suwandi S, Damiri N, Soleha S. 2021b. Jackfruit (Artocarpus heterophyllus), a new host plant of Ceratocystis wilt in South Sumatra, Indonesia. Australas Plant Dis Notes 16: 24. DOI: 10.1007/s13314-021-00435-x.
Pratama R, Muslim A, Suwandi S, Suparman SHK. 2023a. First report of Ceratocystis fimbriata causing wilt disease of soursop in South Sumatra, Indonesia. Biodiversitas 24 (12): 6711-6721. DOI: 10.13057/biodiv/d241233.
Pratama R, Muslim A, Suwandi S. 2023b. First report of new wilt disease on Mangifera indica caused by Ceratocystis fimbriata in Indonesia. Biovalentia 9 (1): 36-40. DOI: 10.24233/biov.9.1.2023.377.
Roman EJ, Esteban LG, de Palacios P, Fernandez FG. 2016. Wood anatomy of Cupressus and its relation to geographical distribution. IAWA J 37 (1): 48-68. DOI: 10.1163/22941932-20160120.
Suwandi S, Irsan C, Hamidson H, Umayah A, Asriyani KD. 2021. Identification and characterization of Ceratocystis fimbriata causing lethal wilt on the Lansium tree in Indonesia. Plant Pathol J 37 (2): 124-136. DOI: 10.5423%2FPPJ.OA.08.2020.0147.
Syazwan SA, Mohd-Farid A, Wan-Muhd-Azrul W-A, Syahmi HM, Zaki AM, Ong SP, Mohamed R. 2022. Survey, identification, and pathogenicity of Ceratocystis fimbriata complex associated with wilt disease on Acacia mangium in Malaysia. Forests 12 (12): 1782. DOI: 10.3390/f12121782.
Xu KC, Zhang RQ, Li J, Bai YH, Yang XD, Sun YX, Huang Q. 2019. Camellia sinensis, a new host plant of Ceratocystis fimbriata from China. Plant Dis 103: 2670. DOI: 10.1094/PDIS-04-19-0802-PDN.
Yunus NM, Maid M, Yong WTL, Anthony FE, Sudin M, Taylor PWJ. 2024. Identification and pathogenicity of Ceratocystis manginecans causing wilt disease on Acacia mangium in Sabah, Malaysia. Biodiversitas 25 (5): 2170-2180. DOI: 10.13057/biodiv/d250535.
Most read articles by the same author(s)
- SITI HERLINDA, MIMMA GUSTIANINGTYAS, SUWANDI SUWANDI, RADIX SUHARJO, JELLY MILINIA PUSPITA SARI, SUPARMAN, HARMAN HAMIDSON, HAMZAH HASYIM, Endophytic fungi from South Sumatra (Indonesia) in seed-treated corn suppressing Spodoptera frugiperda growth , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 11 (2022)