Identification of interspecific hybrid between Jatropha curcas × J. integerrima using morphological and molecular markers
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Abstract. Saptadi D, Asbani N, Heliyanto B, Setiawan A, Sudarsono. 2020. Identification of interspecific hybrid between Jatropha curcas x J. integerrima using morphological and molecular markers. Biodiversitas 21: 814-823. Eight F1 progenies derived from Jatropha curcas × J. integerrima hybridizations were evaluated for their morphological characters and using RAPD, ISSR and SSR markers. Morphological variations among the hybrids were limited and they were intermediate between the Jatropha parents. The eight F1 progenies derived from J. curcas × J. integerrima hybridizations were most probably the interspecific F1 hybrids. The confirmed identity of the progenies as interspecific hybrids between J. curcas × J. integerrima was based on the presence of several phenotypic characters from both parents in the F1 progenies and by similarity of the molecular marker banding patterns among the parents and the F1 progenies. Among the evaluated molecular markers, the ISSR primers and the majority of either RAPD and SSR primers were not able to generate marker for confirming the identity of F1 progenies as interspecific hybrids between J. curcas × J. integerrima. However, the RAPD primer OPC 10 and the SSR primers AF469003, EU099522 and EU586348 were able to generate polymorphic markers in the Jatropha parents and their F1 progenies. Therefore, these four primers were able to generate usable markers for confirming the identity of F1 progenies as interspecific hybrids between J. curcas × J. integerrima. The evaluated interspecific F1 progenies are potentially useful to increase genetic diversity of J. curcas and support its breeding program.
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References
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http://www.funpecrp.com.br/gmr/year2011/vol10-1/pdf/gmr894.pdf
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http://nopr.niscair.res.in/bitstream/123456789/6143/1/IJBT%208%284%29%20384-390.pdf
Divakara, B.N., Upadhyaya, H.D., Wani, S.P., Gowda, C.L.L., 2010. Biology and genetic improvement of Jatropha curcas L.: A review, Applied Energy. https://doi.org/10.1016/j.apenergy.2009.07.013
https://www.sciencedirect.com/science/article/abs/pii/S030626190900302X?via%3Dihub
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https://www.peanutscience.com/doi/full/10.3146/PS07-109.1
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Huang, S.C., Tsai, C.C., Sheu, C.S., 2000. Genetic analysis of Chrysanthemum hybrids based on RAPD molecular markers . Bot. Bull. Acad. Sin. 41, 257–262.
Iqbal, A., Sadaqat, H.A., Khan, A.S., Amjad, M., 2011. Identification of sunflower (Helianthus annuus, Asteraceae) hybrids using simple-sequence repeat markers. Genet. Mol. Res. 10, 102–106.
http://www.funpecrp.com.br/gmr/year2011/vol10-1/pdf/gmr918.pdf
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http://www.funpecrp.com.br/gmr/year2011/vol10-4/pdf/gmr1510.pdf
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https://link.springer.com/article/10.1007%2Fs11295-010-0326-6
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Moose, S.P., Mumm, R.H., 2008. Molecular plant breeding as the foundation for 21st century crop improvement. Plant Physiol. 147, 969–977. https://doi.org/10.1104/pp.108.118232
http://www.plantphysiol.org/content/147/3/969
Perrier, X., Jacquemoud-Collet, J.., 2006. DARwin Softwaree [WWW Document]. URL http://darwin.cirad.fr/darwin
Pollegioni, P., Woeste, K., Mugnozza, G.S., Malvolti, M.E., 2009. Retrospective identification of hybridogenic walnut plants by SSR fingerprinting and parentage analysis. Mol. Breed. 24, 321–335. https://doi.org/10.1007/s11032-009-9294-7
https://link.springer.com/article/10.1007%2Fs11032-009-9294-7
Raju, A.J.S., Ezradanam, V., 2002. Pollination ecology and fruiting behaviour in a monoecious species, Jatropha curcas L. (Euphorbiaceae). Curr. Sci. 83, 1395–1398.
https://www.jstor.org/stable/24106968?seq=1
Rao, S.K., Lakshminarayana, G., 1987. Characteristics and Composition of Six Newer Seeds and the Oils. Fett/Lipid 89, 324–326. https://doi.org/10.1002/lipi.19870890809
https://onlinelibrary.wiley.com/doi/abs/10.1002/lipi.19870890809
Ronis, D.H., Thompson, A.E., Dierig, D.A., Johnson, E.R., 1990. Isozyme verification of intersspecific hybrids of Cuphea. Hortscience 25, 1431–1434.
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&cad=rja&uact=8&ved=2ahUKEwj8-YOh87HmAhUQ7HMBHaWvAG4QFjABegQIAhAB&url=https%3A%2F%2Fjournals.ashs.org%2Fhortsci%2Fdownloadpdf%2Fjournals%2Fhortsci%2F25%2F11%2Farticle-p1431.xml&usg=AOvVaw1s6HzVra9udcphJ8NH0MSw
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https://agrivita.ub.ac.id/index.php/agrivita/article/view/694
Saptadi, D., Hartati, R.S., Setiawan, A., Heliyanto, B., Sudarsono, 2011. Development of Simple Sequence Repeat Markers for Jatropha spp. J. Litrri 17, 140–149. [Indonesian]
http://ejurnal.litbang.pertanian.go.id/index.php/jptip/article/view/2085
Shasany, A.K., Darokar, M.P., Dhawan, S., Gupta, A.K., Gupta, S., Shukla, A.K., Patra, N.K., Khanuja, S.P.S., 2005. Use of RAPD and AFLP markers to identify inter- and intraspecific hybrids of Mentha. J. Hered. 96, 542–549. https://doi.org/10.1093/jhered/esi091
https://academic.oup.com/jhered/article/96/5/542/2187676
Spooner, D., Treuren, R. Van, Vicente, M.C. De, 2005. Molecular markers for genbank management, IPGRI technical bulletin NO.10. https://doi.org/10.1016/j.chemphyslip.2011.03.008
https://www.sciencedirect.com/science/article/abs/pii/S0009308411000417?via%3Dihub
Sudheer, P.D.V.N., Mastan, S.G., Rahman, H., Ravi Prakash, C., Singh, S., Reddy, M.P., 2011. Cross species amplification ability of novel microsatellites isolated from Jatropha curcas and genetic relationship with sister taxa?: Cross species amplification and genetic relationship of Jatropha using novel microsatellites. Mol. Biol. Rep. 38, 1383–1388. https://doi.org/10.1007/s11033-010-0241-9
https://link.springer.com/article/10.1007%2Fs11033-010-0241-9
Sudheer Pamidiamarri, D.V.N., Pandya, N., Reddy, M.P., Radhakrishnan, T., 2009. Comparative study of interspecific genetic divergence and phylogenic analysis of genus Jatropha by RAPD and AFLP?: GGenetic divergence and phylogenic analysis of genus Jatropha. Mol. Biol. Rep. 36, 901–907. https://doi.org/10.1007/s11033-008-9261-0
https://link.springer.com/article/10.1007%2Fs11033-008-9261-0
Sujatha, M., Prabakaran, a J., 2003. New ornamental jatropha through interspecific hybridization. Genet. Resour. Crop Evol. 50, 75–82.
https://link.springer.com/article/10.1023/A:1022961028064
Tyagi, B., Ahmad, T., JR, B., 1992. Cytology, genetics and breeding of commercially important Mentha species. Curr Res Med Aromat Plants 14, 51–56.
Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S. V, 1990. DNA polymorphisms amplified by arbitrary primers are usefil as genetic markers 18, 6531–6535.
images.biomedsearch.com/1979162/nar00206-0065.pdf?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIAIBOKHYOLP4MBMRGQ%2F20191213%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Date=20191213T000000Z&X-Amz-Expires=172800&X-Amz-SignedHeaders=host&X-Amz-Signature=9880b0804fee765a912711e5dec00b1dc627cbca0d8f7722c9974be6fb455ba9
Wu, M., Jia, X., Tian, L., Lv, B., 2010. Rapid and Reliable Purity Identification of F1 Hybrids of Maize (Zea may L.) Using SSR Markers. Mol. Plant Breed. 4, 381–384. https://doi.org/10.5376/mgg.2010.01.0001
Yi, C., Zhang, S., Liu, X., Bui, H.T., Hong, Y., 2010. Does epigenetic polymorphism contribute to phenotypic variances in Jatropha curcas L.? BMC Plant Biol 10, 259. https://doi.org/10.1186/1471-2229-10-259
https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-10-259
Asbani, N., 2008. Interspesific Hybridization Of Jatropha curcas L. And Jatropha integerrima Jaeq. For Mites And Thrips Resistant Crops. In: 4th National Workshop: Acceleration Of Physic Nut Technology Innovation Toward Energy Self Sufficiency. Indonesian Sweetener and Fiber Crops Research Institute, 6 November 2008. [Indonesian]
http://balittas.litbang.pertanian.go.id/images/pdf/jp396.pdf
Conceição, L.D.H.C.S., Belo, G.O., Souza, M.M., Santos, S.F., Cerqueira-Silva, C.B.M., Corrêa, R.X., 2011. Confirmation of cross-fertilization using molecular markers in ornamental passion flower hybrids. Genet. Mol. Res. 10, 47–52. https://doi.org/10.4238/vol10-1gmr894
http://www.funpecrp.com.br/gmr/year2011/vol10-1/pdf/gmr894.pdf
Dhillon, R.S., Hooda, M.S., Jattan, M., Chawla, V., Bhardwaj, M., Goyal, S.C., 2009. Development and molecular characterization of interspecific hybrids of Jatropha curcas × J. integerrima. Indian J. Biotechnol. 8, 384–390.
http://nopr.niscair.res.in/bitstream/123456789/6143/1/IJBT%208%284%29%20384-390.pdf
Divakara, B.N., Upadhyaya, H.D., Wani, S.P., Gowda, C.L.L., 2010. Biology and genetic improvement of Jatropha curcas L.: A review, Applied Energy. https://doi.org/10.1016/j.apenergy.2009.07.013
https://www.sciencedirect.com/science/article/abs/pii/S030626190900302X?via%3Dihub
Doyle, J., 1990. Isolation of plant DNA from fresh tissue. Focus (Madison). 12, 13–15.
Gomez, S.M., Denwar, N.N., Ramasubramanian, T., Simpson, C.E., Burow, G., Burke, J.J., Puppala, N., Burow, M.D., 2008. Identification of Peanut Hybrids Using Microsatellite Markers and Horizontal Polyacrylamide Gel Electrophoresis. Peanut Sci. 35, 123–129. https://doi.org/10.3146/PS07-109.1
https://www.peanutscience.com/doi/full/10.3146/PS07-109.1
Heller, J., 1996. Physic nut. Jatropha curcas L. Promoting the conservation and use of underutilized and neglected crops. 1.
https://www.bioversityinternational.org/fileadmin/_migrated/uploads/tx_news/Physic_nut__Jatropha_curcas_L._161.pdf
Hirose, T., Ujihara, A., Kitabayashi, H., Minami, M., 1993. Morphology and identification by interspecific hybrids in buckwheats isozyme analysis 13, 25–30.
Huang, S.C., Tsai, C.C., Sheu, C.S., 2000. Genetic analysis of Chrysanthemum hybrids based on RAPD molecular markers . Bot. Bull. Acad. Sin. 41, 257–262.
Iqbal, A., Sadaqat, H.A., Khan, A.S., Amjad, M., 2011. Identification of sunflower (Helianthus annuus, Asteraceae) hybrids using simple-sequence repeat markers. Genet. Mol. Res. 10, 102–106.
http://www.funpecrp.com.br/gmr/year2011/vol10-1/pdf/gmr918.pdf
Kang, J.H., Kim, Y.K., Park, J.Y., An, C.M., Nam, M.M., Byun, S.G., Lee, B.I., Lee, J.H., Choi, T.J., 2011. Microsatellite analysis as a tool for discriminating an interfamily hybrid between olive flounder and starry flounder. Genet. Mol. Res. 10, 2786–2794. https://doi.org/10.4238/2011.October.31.16
http://www.funpecrp.com.br/gmr/year2011/vol10-4/pdf/gmr1510.pdf
Kumar Yadav, H., Ranjan, A., Asif, M.H., Mantri, S., Sawant, S. V., Tuli, R., 2011. EST-derived SSR markers in Jatropha curcas L.: Development, characterization, polymorphism, and transferability across the species/genera. Tree Genet. Genomes 7, 207–219. https://doi.org/10.1007/s11295-010-0326-6
https://link.springer.com/article/10.1007%2Fs11295-010-0326-6
Lakshminarayana, M., Sujatha, M., 2001. Screening of Jatropha species against the major defoliators of castor (Ricinus communis L.). J. Oilseeds Res. 18, 228–230.
Moose, S.P., Mumm, R.H., 2008. Molecular plant breeding as the foundation for 21st century crop improvement. Plant Physiol. 147, 969–977. https://doi.org/10.1104/pp.108.118232
http://www.plantphysiol.org/content/147/3/969
Perrier, X., Jacquemoud-Collet, J.., 2006. DARwin Softwaree [WWW Document]. URL http://darwin.cirad.fr/darwin
Pollegioni, P., Woeste, K., Mugnozza, G.S., Malvolti, M.E., 2009. Retrospective identification of hybridogenic walnut plants by SSR fingerprinting and parentage analysis. Mol. Breed. 24, 321–335. https://doi.org/10.1007/s11032-009-9294-7
https://link.springer.com/article/10.1007%2Fs11032-009-9294-7
Raju, A.J.S., Ezradanam, V., 2002. Pollination ecology and fruiting behaviour in a monoecious species, Jatropha curcas L. (Euphorbiaceae). Curr. Sci. 83, 1395–1398.
https://www.jstor.org/stable/24106968?seq=1
Rao, S.K., Lakshminarayana, G., 1987. Characteristics and Composition of Six Newer Seeds and the Oils. Fett/Lipid 89, 324–326. https://doi.org/10.1002/lipi.19870890809
https://onlinelibrary.wiley.com/doi/abs/10.1002/lipi.19870890809
Ronis, D.H., Thompson, A.E., Dierig, D.A., Johnson, E.R., 1990. Isozyme verification of intersspecific hybrids of Cuphea. Hortscience 25, 1431–1434.
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&cad=rja&uact=8&ved=2ahUKEwj8-YOh87HmAhUQ7HMBHaWvAG4QFjABegQIAhAB&url=https%3A%2F%2Fjournals.ashs.org%2Fhortsci%2Fdownloadpdf%2Fjournals%2Fhortsci%2F25%2F11%2Farticle-p1431.xml&usg=AOvVaw1s6HzVra9udcphJ8NH0MSw
Saptadi, D., Hartati, R.S., Setiawan, A., Heliyanto, B., Sudarsono, 2017. Genetic Diversity of Indonesian Physic Nut ( J . curcas ) Based on Molecular Marker 39, 160–171. http://doi.org/10.17503/agrivita.v39i2.694
https://agrivita.ub.ac.id/index.php/agrivita/article/view/694
Saptadi, D., Hartati, R.S., Setiawan, A., Heliyanto, B., Sudarsono, 2011. Development of Simple Sequence Repeat Markers for Jatropha spp. J. Litrri 17, 140–149. [Indonesian]
http://ejurnal.litbang.pertanian.go.id/index.php/jptip/article/view/2085
Shasany, A.K., Darokar, M.P., Dhawan, S., Gupta, A.K., Gupta, S., Shukla, A.K., Patra, N.K., Khanuja, S.P.S., 2005. Use of RAPD and AFLP markers to identify inter- and intraspecific hybrids of Mentha. J. Hered. 96, 542–549. https://doi.org/10.1093/jhered/esi091
https://academic.oup.com/jhered/article/96/5/542/2187676
Spooner, D., Treuren, R. Van, Vicente, M.C. De, 2005. Molecular markers for genbank management, IPGRI technical bulletin NO.10. https://doi.org/10.1016/j.chemphyslip.2011.03.008
https://www.sciencedirect.com/science/article/abs/pii/S0009308411000417?via%3Dihub
Sudheer, P.D.V.N., Mastan, S.G., Rahman, H., Ravi Prakash, C., Singh, S., Reddy, M.P., 2011. Cross species amplification ability of novel microsatellites isolated from Jatropha curcas and genetic relationship with sister taxa?: Cross species amplification and genetic relationship of Jatropha using novel microsatellites. Mol. Biol. Rep. 38, 1383–1388. https://doi.org/10.1007/s11033-010-0241-9
https://link.springer.com/article/10.1007%2Fs11033-010-0241-9
Sudheer Pamidiamarri, D.V.N., Pandya, N., Reddy, M.P., Radhakrishnan, T., 2009. Comparative study of interspecific genetic divergence and phylogenic analysis of genus Jatropha by RAPD and AFLP?: GGenetic divergence and phylogenic analysis of genus Jatropha. Mol. Biol. Rep. 36, 901–907. https://doi.org/10.1007/s11033-008-9261-0
https://link.springer.com/article/10.1007%2Fs11033-008-9261-0
Sujatha, M., Prabakaran, a J., 2003. New ornamental jatropha through interspecific hybridization. Genet. Resour. Crop Evol. 50, 75–82.
https://link.springer.com/article/10.1023/A:1022961028064
Tyagi, B., Ahmad, T., JR, B., 1992. Cytology, genetics and breeding of commercially important Mentha species. Curr Res Med Aromat Plants 14, 51–56.
Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S. V, 1990. DNA polymorphisms amplified by arbitrary primers are usefil as genetic markers 18, 6531–6535.
images.biomedsearch.com/1979162/nar00206-0065.pdf?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIAIBOKHYOLP4MBMRGQ%2F20191213%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Date=20191213T000000Z&X-Amz-Expires=172800&X-Amz-SignedHeaders=host&X-Amz-Signature=9880b0804fee765a912711e5dec00b1dc627cbca0d8f7722c9974be6fb455ba9
Wu, M., Jia, X., Tian, L., Lv, B., 2010. Rapid and Reliable Purity Identification of F1 Hybrids of Maize (Zea may L.) Using SSR Markers. Mol. Plant Breed. 4, 381–384. https://doi.org/10.5376/mgg.2010.01.0001
Yi, C., Zhang, S., Liu, X., Bui, H.T., Hong, Y., 2010. Does epigenetic polymorphism contribute to phenotypic variances in Jatropha curcas L.? BMC Plant Biol 10, 259. https://doi.org/10.1186/1471-2229-10-259
https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-10-259
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