Short Communication: Diversity of culturable epiphytic bacteria isolated from seagrass (Halodule uninervis) in Thailand and their preliminary antibacterial activity
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Abstract. Boontanom P, Chantarasiri A. 2020. Short Communication: Diversity of culturable epiphytic bacteria isolated from seagrass (Halodule uninervis) in Thailand and their preliminary antibacterial activity. Biodiversitas 21: 2907-2913. Epiphytic bacteria are symbiotic bacteria that live on the surface of seagrasses. This study presents the diversity of culturable epiphytic bacteria associated with the Kuicheai seagrass (H. uninervis) collected from Rayong Province in Eastern Thailand. Nine epiphytic isolates were identified into four phylogenetical genera based on their 16S rRNA nucleotide sequence analyses. They are considered firmicutes in the genera of Planomicrobium, Paenibacillus and Bacillus, and proteobacteria in the genus of Oceanimonas. Three species of epiphytic bacteria preliminarily exhibited antibacterial activity against the human pathogenic Staphylococcus aureus using the perpendicular streak method. The knowledge obtained from this study increases understanding the diversity of seagrass-associated bacteria in Thailand and suggests the utilization of these bacteria for further pharmaceutical applications.
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References
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Arahal DR, Lucena T, Macián MC, Ruvira, MA, González JM, Lekumberri I, Pinhassi J, Pujalte MJ. 2016. Marinomonas blandensis sp. nov., a novel marine gammaproteobacterium. International Journal of Systematic and Evolutionary Microbiology 66: 5544-5549.
Armstrong E, Yan L, Boyd KG, Wright PC, Burgess JG. 2001. The symbiotic role of marine microbes on living surfaces. Hydrobiologia 461(1): 37-40.
Celdrán D, Espinosa E, Sánchez-Amat A, Marín A. 2012. Effects of epibiotic bacteria on leaf growth and epiphytes of the seagrass Posidonia oceanica. Marine Ecology Progress Series 456: 21-27.
Cita YP, Suhermanto A, Radjasa OK, Sudharmono P. 2017. Antibacterial activity of marine bacteria isolated from sponge Xestospongia testudinaria from Sorong, Papua. Asian Pacific Journal of Tropical Biomedicine 7(5): 450-454.
Cristianawati O, Sibero MT, Ayuningrum D, Nuryadi H, Syafitri E, Riniarsih I, Radjasa OK. 2019. Screening of antibacterial activity of seagrass-associated bacteria from the North Java Sea, Indonesia against multidrug-resistant bacteria. AACL Bioflux 12(4): 1054-1064.
Ferbiyanto A, Rusmana I, Raffiudin R. 2015. Characterization and identification of cellulolytic bacteria from gut of worker Macrotermes gilvus. HAYATI Journal of Biosciences 22(4): 197-200.
Gouy M, Guindon S, Gascuel O. 2010. SeaView version 4: A multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Molecular Biology and Evolution 27(2): 221-224.
Lorentz RH, Ártico S, da Silveira AB, Einsfeld A, Corção G. 2006. Evaluation of antimicrobial activity in Paenibacillus spp. strains isolated from natural environment. Letters in Applied Microbiology 43: 541-547.
Nugraheni SA, Khoeri MM, Kusmita L, Widyastuti Y, Radjasa OK. 2010. Characterization of carotenoid pigments from bacterial symbionts of seagrass Thalassia hemprichii. Journal of Coastal Development 14(1): 51-60.
Orth RJ, Carruthers TJB, Dennison WC, Duarte CM, Fourqurean JW, Heck KL, Hughes AR, Kendrick GA, Kenworthy WJ, Olyarnik S, Short FT, Waycott M, Williams SL. 2006. A global crisis for seagrass ecosystems. BioScience 56(12): 987-996.
Saggese A, Culurciello R, Casillo A, Corsaro MM, Ricca E, Baccigalupi L. 2018. A marine isolate of Bacillus pumilus secretes a pumilacidin active against Staphylococcus aureus. Marine Drugs 16: DOI:10.3390/md16060180
Simon L, Škraban J, Kyrpides NC, Woyke T, Shapiro N, Cleenwerck I, Vandamme P, Whitman WB, Tr?ek J. 2017. Paenibacillus aquistagni sp. nov., isolated from an artificial lake accumulating industrial wastewater. Antonie van Leeuwenhoek 110: 1189-1197.
Singer RS, Finch R, Wegener HC, Bywater R, Walters J, Lipsitch M. 2003. Antibiotic resistance-the interplay between antibiotic use in animals and human beings. The Lancet Infectious Diseases 3: 47-51.
Sirimungkara A, Laongmanee W, Paibulkichakul B, Yuvanatemiya V, Leadprathom N. 2016. Seasonal effect on distribution of seagrass in coastal of Rayong Province. Khon Kaen Agriculture Journal 44: 745-751.
Tarquinio F, Hyndes GA, Laverock B, Koenders A, Säwström C. 2019. The seagrass holobiont: understanding seagrass-bacteria interactions and their role in seagrass ecosystem functioning. FEMS Microbiology Letters 366(6): DOI: https://doi.org/10.1093/femsle/fnz057
Ting ASY, Tan SH, Wai MK. 2009. Isolation and characterization of actinobacteria with antibacterial activity from soil and rhizosphere soil. Australian Journal of Basic and Applied Sciences 3(4): 4053-4059.
Ugarelli K, Chakrabarti S, Laas P, Stingl U. 2017. The seagrass holobiont and its microbiome. Microorganisms 5: DOI: 10.3390/microorganisms5040081
Wanna C, Phongpha C. 2018. Species diversity of aquatic fauna in seagrass at Rockgarden Village, Rayong Province. Suan Sunandha Science and Technology Journal 5(1): 5-11.
Webster NS, Taylor MW. 2011. Marine sponges and their microbial symbionts: love and other relationships. Environmental Microbiology 14: 335-346.
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