Assessment of genetic diversity among soursop (Annona muricata) populations from Java, Indonesia using RAPD markers

Abstract

Suratman, Ari Pitoyo, Sri Mulyani, Suranto. 2015. Assessment of genetic diversity among soursop (Annona muricata)
populations from Java, Indonesia using RAPD markers. Biodiversitas 16: 247-253. The objective of this study was to determine genetic
diversity of the soursop (Annona muricata L.) populations from Java (Indonesia) using RAPD markers. A total of 70 individuals were
collected from 7 soursop populations, distributed along the geographical range of natural distribution in Java. Genetic diversity was
estimated by RAPD technique using 6 arbitrary selected primers. Those primers produced 151 polymorphic bands with the percentage
of polymorphism for each primer ranged from 95% to 100%. The genetic diversity value (h) within each population ranged from 0.0418
to 0.0525. The highest h value (0.0525) was found in the KRA population whereas the lowest h value was observed in the PCT
population. The highest genetic distance value (0.0410) was observed in SKH-GKD populations pair whereas the lowest genetic
distance value (0.02448) was estimated in KRA-PCT populations pair. Based on the dendogram, the seven soursop populations were
segregated into three major clusters. The first cluster consisted of SKH, KRA, PCT, NGW, and KPG populations. The GKD population
was then grouped into second cluster. In the third cluster, the BGR population was grouped separately and more genetically distant than
the others. However, the relationship dendrogram showed that the grouping did not always indicate the geographical origins similarity,
but possibly showed the genetic similarity.