Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants
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Abstract. Khaeruddin, Junaedi, Hastuti. 2020. Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants. Biodiversitas 21: 5718-5722. Imported purebred chickens are becoming more popular and a regular staple in Indonesia. Therefore, it is necessary to strengthen conservation efforts to preserve Indonesian chickens, one of which is by means of sperm cryopreservation. This study aimed to determine the effects of the addition of DMSO and different concentrations of ethylene glycol to a Ringer’s lactate egg yolk (RLY)-or coconut water egg yolk (CWY)-based extender on the quality of frozen-thawed Indonesian chicken sperm. This study was used nine Indonesian native roosters about 20 months of age. The semen extenders used in this study were RLY + DMSO 7%, RLY + ethylene glycol 3%, RLY + ethylene glycol 5%, RLY + ethylene glycol 7%, CWY + DMSO 7%, CWY + ethylene glycol 3%, CWY + ethylene glycol 5% and CWY + ethylene glycol 7%. Liquid semen was packaged in 0.25 mL straw, then cooled at 5oC for 2 hours, frozen at 5 cm above liquid nitrogen for 10 minutes, following stored in a liquid nitrogen container for 24 hours. The semen straws were thawed at 37oC for 30 seconds. Statistical analysis for multiple comparisons was performed as a completely randomized design with eight treatment levels and seven replications. The results showed that there were no differences in sperm motility, recovery rate, and abnormality between extenders after the freeze-thaw process. Whereas, RLY + DMSO 7% was the highest sperm viability.
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References
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Ameen SA, Opayemi OS, Ajayi JA, Adediwura MA. 2014. Evaluation of semen quality of five different cockerel breed used in poultry industry in Nigeria. Journal of Environmental Issues and Agriculture in Developing Countries 6(1): 30-36.
Awad MM. 2011. Effect of some permeating cryoprotectants on CASA motility results in cryopreserved bull spermatozoa. Anim Reprod Sci 123: 157-162. https://doi.org/ 10.1016/j.anireprosci.2011.01.003.
Blesbois E, Grasseau I, Seigneurin F, Mignon-Grasteau S, Saint Jalme M, Mialon-Richard MM. 2008. Predictors of semen cryopreservation in chickens. Theriogenology 69: 252-261. https://doi.org/10.1016/j.theriogenology.2007.09.019.
Gerzilov P. 2010. Influence of various cryoprotectants on the sperm mobility of Muscovy semen before and after cryopreservation. Agricultural Science And Technology 2(2): 57 – 60.
Gillmore JA, Liu J, Woods EJ, Peter AT, Crister JK. 2000. Cryoprotective agent and temperature effects on human sperm membrane permeabilities: convergence of theoretical and empirical approaches for optimal cryopreservation methods. Hum Reprod 15: 335-343. https://doi.org/10.1093/humrep/15.2.335.
Woelders H, Zuidberg CA, Hiemstra SJ. 2006. Animal genetic resources conservation in the Netherlands and Europe: poultry perspective. Poult Sci 85: 216–222. https://doi.org/10.1093/ps/85.2.216.
Bui TVL, Ross IL, Jakob G, Hankamer B. 2013. Impact of procedural steps and cryopreservation agents in the cryopreservation of chlorophyte microalgae. Plos One 8(11). https://doi.org/10.1371/journal.pone.0078668
Chaytor JL, Tokarew JM, Wu LK, Leclère M, Tam RY, Capicciotti Cj, Guolla L, von Moos E, Findlay CS, Allan DS, Ben RN. 2012. Inhibiting ice recrystallization and optimization of cell viability after cryopreservation. Glycobiology 22(1): 123-133.
Pegg DE. 2015. Principles of cryopreservation. Methods Mol Biol. 1257: 3-19. https://doi.org/ 10.1007/978-1-4939-2193-5_1
Silva ECB, Cajueiro JFP, Silva SV, Vidal AH, Soares PC, Guerra MMP. 2012. In vitro evaluation of ram sperm frozen with glycerol, ethylene glycol or acetamide. Anim Reprod Sci 132(3-4): 155-158. https://doi.org/10.1016/j.anireprosci.2012.05.014.
Rota A, Milani C, Cabianca G, Martini M. 2006. Comparison between glycerol and ethylene glycol for dog semen cryopreservation. Theriogenology 65(9): 1848-1858. https://doi.org/10.1016/j.theriogenology.2005.10.015
Sexton TJ. 1973. Effect of various cryoprotective agents on the viability and reproductive efficiency of chicken spermatozoa. Poult Sci 52: 1353-1357. DOI
Swelum AA, Mansour HA, Elsayed AA, Amer HAA. 2011. Comparing ethylene glycol with glycerol for cryopreservation of buffalo bull semen in egg-yolk containing extenders. Theriogenology 76(5): 833-842. https://doi.org/10.1016/j.theriogenology.2011.04.015
Alcay S, Ustuner B, Nur Z. 2016. Effect of low molecular weight cryoprotectants on the post-thaw ram sperm quality and fertilizing ability. Small Ruminant Res. 136: 59-64. https://doi.org/10.1016/j.smallrumres.2016.01.009
Bhattacharya S, Prajapati BG. 2016. A review on cryoprotectant and its modern implication in cryonics. Asian Journal of Pharmaseutics. 10(3): 154-159. http://dx.doi.org/10.22377/ajp.v10i3.721
Junaedi, Arifiantini RI, Sumantri C, Gunawan A. 2016a. The use of dimethyl sulfoxide as cryoprotective agent for native chicken frozen semen. JVeteriner 17(2): 300-308. 10.19087/jveteriner.2016.17.2.300
Junaedi, Arifiantini RI, Sumantri C, Gunawan A. 2016b. Use pf glycerol as cryoprotectants in freezing Sentul chicken semen. CJAH 1(2): 6-13. https://doi.org/10.31327/chalaza.v1i2.165
Junaedi, Arifiantini RI, Sumantri C. 2017. The quality of Kampung broiler (KB) chicken frozen semen with DMA concentrations on yolk lactate ringer diluent. CJAH 2(2): 19-24. https://doi.org/10.31327/chalaza.v2i2.292
Leibo SP, Pool TB. 2011. The principal variables of cryopreservation: solutions, temperatures, and rate changes. Fertil Steril 96(2): 269-276. https://doi.org/10.1016/j.fertnstert.2011.06.065
Long JA. 2006. Avian semen cryopreservation : what are the biological challenges?. Poult Sci 85: 232-236. https://doi.org/10.1093/ps/85.2.232.
Martini WZ, Cortez DS, Dubick MA. 2013. Comparisons of normal saline and lactated ringer’s resuscitation on hemodynamics, metabolic responses, and coagulation in pigs after severe hemorrhagic shock. Scand J Trauma Resus 21:86. https://doi.org/10.1186/1757-7241-21-86
Mphaphathi ML, Seshoka MM, Luseba D, Sutherland B, Nedambale TL. 2016. The characterisation and cryopreservation of Venda chicken semen. Asian Pac J Reprod 5(2): 132-139. http://dx.doi.org/10.1016/j.apjr.2016.01.009
das Neves CM, Romano FR, Filho SG. 2019. New ringer’s lactate gel formulation on nasal comfort and humidification. Braz J Otorhinolaryngol 85(6):746-752. https://doi.org/10.1016/j.bjorl.2018.07.004
Fujita Y, Nishimura M, Komori NW, Wada T, Shirakawa C, Takenawa T, Sawamoto O, Doi M. 2020. A pair cell preservation solutions for therapy with human adipose tissue-derived mesenchymal stromal cells. Regen Ther 14: 95-102. https://doi.org/10.1016/j.reth.2019.10.004
Pelàez J, Long JA. 2008. Characterizing the Glycocalyx of poultry semen: II In vitro storage of turkey semen and mobility phenotype affects the carbohydrate component of sperm membrane glycoconjugates. J Androl 29: 431-439. https://doi.org/10.2164/jandrol.107.004259
Rashid A, Qistina MNN. 2015 Preliminary evaluation on the use of coconut water as semen diluent for local Kampung cockerel. In: Yaakub HB, Saad WZ, Akit H, Alimon AR (eds). 2nd Asean Regional Conference on Animal Production (ARCAP) and 36th Malaysian Society of Animal Production (MSAP) Annual Conference. MSAP, Port Dicson, Negeri Sembilan, 1-3 June 2015. [Malaysia]
Seshoka MM , Mphaphathi ML, Nedambale TL. 2016. Comparison of four different permitting and combination of two best cryoprotectants on freezing Nguni sperm evaluated with the aid of computer aided sperm analysis. Cryobiology 72 :232-238. http://dx.doi.org/10.1016/j.cryobiol.2016.04.001
Ushiyama A, Ishikawa N, Tajima A, Asano A. 2016. Comparison of membrane characteristics between freshly ejaculated and cryopreserved sperm in the chicken. J Poult Sci 53: 305-312. https://doi.org/10.2141/ jpsa.0160043
Feyisa SG, Park YH, Kim YM, Lee BR, Jung KM, Choi SB, Cho CY, Han JY. 2018. Morphological defects of sperm and their association with motility, fertility, and hatchability in four Korean native chicken breeds. Asian-Australas J Anim Sci 31(8):1160-1168. https://doi.org/10.5713/ajas.17.0626
Ameen SA, Opayemi OS, Ajayi JA, Adediwura MA. 2014. Evaluation of semen quality of five different cockerel breed used in poultry industry in Nigeria. Journal of Environmental Issues and Agriculture in Developing Countries 6(1): 30-36.
Awad MM. 2011. Effect of some permeating cryoprotectants on CASA motility results in cryopreserved bull spermatozoa. Anim Reprod Sci 123: 157-162. https://doi.org/ 10.1016/j.anireprosci.2011.01.003.
Blesbois E, Grasseau I, Seigneurin F, Mignon-Grasteau S, Saint Jalme M, Mialon-Richard MM. 2008. Predictors of semen cryopreservation in chickens. Theriogenology 69: 252-261. https://doi.org/10.1016/j.theriogenology.2007.09.019.
Gerzilov P. 2010. Influence of various cryoprotectants on the sperm mobility of Muscovy semen before and after cryopreservation. Agricultural Science And Technology 2(2): 57 – 60.
Gillmore JA, Liu J, Woods EJ, Peter AT, Crister JK. 2000. Cryoprotective agent and temperature effects on human sperm membrane permeabilities: convergence of theoretical and empirical approaches for optimal cryopreservation methods. Hum Reprod 15: 335-343. https://doi.org/10.1093/humrep/15.2.335.
Woelders H, Zuidberg CA, Hiemstra SJ. 2006. Animal genetic resources conservation in the Netherlands and Europe: poultry perspective. Poult Sci 85: 216–222. https://doi.org/10.1093/ps/85.2.216.
Bui TVL, Ross IL, Jakob G, Hankamer B. 2013. Impact of procedural steps and cryopreservation agents in the cryopreservation of chlorophyte microalgae. Plos One 8(11). https://doi.org/10.1371/journal.pone.0078668
Chaytor JL, Tokarew JM, Wu LK, Leclère M, Tam RY, Capicciotti Cj, Guolla L, von Moos E, Findlay CS, Allan DS, Ben RN. 2012. Inhibiting ice recrystallization and optimization of cell viability after cryopreservation. Glycobiology 22(1): 123-133.
Pegg DE. 2015. Principles of cryopreservation. Methods Mol Biol. 1257: 3-19. https://doi.org/ 10.1007/978-1-4939-2193-5_1
Silva ECB, Cajueiro JFP, Silva SV, Vidal AH, Soares PC, Guerra MMP. 2012. In vitro evaluation of ram sperm frozen with glycerol, ethylene glycol or acetamide. Anim Reprod Sci 132(3-4): 155-158. https://doi.org/10.1016/j.anireprosci.2012.05.014.
Rota A, Milani C, Cabianca G, Martini M. 2006. Comparison between glycerol and ethylene glycol for dog semen cryopreservation. Theriogenology 65(9): 1848-1858. https://doi.org/10.1016/j.theriogenology.2005.10.015
Sexton TJ. 1973. Effect of various cryoprotective agents on the viability and reproductive efficiency of chicken spermatozoa. Poult Sci 52: 1353-1357. DOI
Swelum AA, Mansour HA, Elsayed AA, Amer HAA. 2011. Comparing ethylene glycol with glycerol for cryopreservation of buffalo bull semen in egg-yolk containing extenders. Theriogenology 76(5): 833-842. https://doi.org/10.1016/j.theriogenology.2011.04.015
Alcay S, Ustuner B, Nur Z. 2016. Effect of low molecular weight cryoprotectants on the post-thaw ram sperm quality and fertilizing ability. Small Ruminant Res. 136: 59-64. https://doi.org/10.1016/j.smallrumres.2016.01.009
Bhattacharya S, Prajapati BG. 2016. A review on cryoprotectant and its modern implication in cryonics. Asian Journal of Pharmaseutics. 10(3): 154-159. http://dx.doi.org/10.22377/ajp.v10i3.721
Junaedi, Arifiantini RI, Sumantri C, Gunawan A. 2016a. The use of dimethyl sulfoxide as cryoprotective agent for native chicken frozen semen. JVeteriner 17(2): 300-308. 10.19087/jveteriner.2016.17.2.300
Junaedi, Arifiantini RI, Sumantri C, Gunawan A. 2016b. Use pf glycerol as cryoprotectants in freezing Sentul chicken semen. CJAH 1(2): 6-13. https://doi.org/10.31327/chalaza.v1i2.165
Junaedi, Arifiantini RI, Sumantri C. 2017. The quality of Kampung broiler (KB) chicken frozen semen with DMA concentrations on yolk lactate ringer diluent. CJAH 2(2): 19-24. https://doi.org/10.31327/chalaza.v2i2.292
Leibo SP, Pool TB. 2011. The principal variables of cryopreservation: solutions, temperatures, and rate changes. Fertil Steril 96(2): 269-276. https://doi.org/10.1016/j.fertnstert.2011.06.065
Long JA. 2006. Avian semen cryopreservation : what are the biological challenges?. Poult Sci 85: 232-236. https://doi.org/10.1093/ps/85.2.232.
Martini WZ, Cortez DS, Dubick MA. 2013. Comparisons of normal saline and lactated ringer’s resuscitation on hemodynamics, metabolic responses, and coagulation in pigs after severe hemorrhagic shock. Scand J Trauma Resus 21:86. https://doi.org/10.1186/1757-7241-21-86
Mphaphathi ML, Seshoka MM, Luseba D, Sutherland B, Nedambale TL. 2016. The characterisation and cryopreservation of Venda chicken semen. Asian Pac J Reprod 5(2): 132-139. http://dx.doi.org/10.1016/j.apjr.2016.01.009
das Neves CM, Romano FR, Filho SG. 2019. New ringer’s lactate gel formulation on nasal comfort and humidification. Braz J Otorhinolaryngol 85(6):746-752. https://doi.org/10.1016/j.bjorl.2018.07.004
Fujita Y, Nishimura M, Komori NW, Wada T, Shirakawa C, Takenawa T, Sawamoto O, Doi M. 2020. A pair cell preservation solutions for therapy with human adipose tissue-derived mesenchymal stromal cells. Regen Ther 14: 95-102. https://doi.org/10.1016/j.reth.2019.10.004
Pelàez J, Long JA. 2008. Characterizing the Glycocalyx of poultry semen: II In vitro storage of turkey semen and mobility phenotype affects the carbohydrate component of sperm membrane glycoconjugates. J Androl 29: 431-439. https://doi.org/10.2164/jandrol.107.004259
Rashid A, Qistina MNN. 2015 Preliminary evaluation on the use of coconut water as semen diluent for local Kampung cockerel. In: Yaakub HB, Saad WZ, Akit H, Alimon AR (eds). 2nd Asean Regional Conference on Animal Production (ARCAP) and 36th Malaysian Society of Animal Production (MSAP) Annual Conference. MSAP, Port Dicson, Negeri Sembilan, 1-3 June 2015. [Malaysia]
Seshoka MM , Mphaphathi ML, Nedambale TL. 2016. Comparison of four different permitting and combination of two best cryoprotectants on freezing Nguni sperm evaluated with the aid of computer aided sperm analysis. Cryobiology 72 :232-238. http://dx.doi.org/10.1016/j.cryobiol.2016.04.001
Ushiyama A, Ishikawa N, Tajima A, Asano A. 2016. Comparison of membrane characteristics between freshly ejaculated and cryopreserved sperm in the chicken. J Poult Sci 53: 305-312. https://doi.org/10.2141/ jpsa.0160043
Feyisa SG, Park YH, Kim YM, Lee BR, Jung KM, Choi SB, Cho CY, Han JY. 2018. Morphological defects of sperm and their association with motility, fertility, and hatchability in four Korean native chicken breeds. Asian-Australas J Anim Sci 31(8):1160-1168. https://doi.org/10.5713/ajas.17.0626
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