Short Communication: In vitro antimicrobial and antimalarial screening of a crude extract of Streptomyces sp. AB8 isolated from Lapindo Mud Volcano Area, Sidoarjo, Indonesia
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ACHMAD ARIFIYANTO
♥
https://orcid.org/0000-0002-0224-6309
ENDAH SETYANINGRUM
NISMAH NUKMAL
TITIK NUR AENY
Abstract
Abstract. Arifiyanto A, Setyaningrum E, Nukmal N, Aeny TN. 2021. Short Communication: In vitro antimicrobial and antimalarial screening of a crude extract of Streptomyces sp. AB8 isolated from Lapindo Mud Volcano Area, Sidoarjo, Indonesia. Biodiversitas 22: 2817-2823. Streptomyces is a potential bacterial genus that has been investigated extensively as a source of natural microbial compounds. Its potential metabolites have been widely developed for pharmaceutical, pathogen control, and other applications in agriculture. This study aimed to determine the ability of the Streptomyces sp AB8 crude extract in inhibiting Plasmodium and pathogenic microbes. Streptomyces was cultured on Gause synthetic media for 10 days. The fermented broth culture media has dissolved in a 1:1 mixture of ethyl acetate and methanol. Biochemical characterization of this isolate has carried out using the standard methods. In-vitro antimalarial activity assay was performed using a chloroquine-sensitive Plasmodium falciparum strain 3D7. Fresh type O-positive human erythrocytes were suspended at 4 percent hematocrit in a complete medium to maintain culture. The inhibitory concentration (IC50) was determined using probit analysis. The results showed the extract of Streptomyces sp. AB8 contains phenolic and alkaloids. Streptomyces sp. AB8 extract can inhibit Dickeya zeae N-Unila 5, Dickeya zeae N-Unila 10, Aspergillus sp IK3, and Escherichia coli growth. The results also showed that the ICs0 value of extract against P. falciparum 3D7 was 17.56 ug/mL. Further research was needed to determine the types of purified bioactive compounds and their bioactivity.
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References
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Rani, R., Arora, S., Kaur, J., & Manhas, R. K. (2018). Phenolic compounds as antioxidants and chemopreventive drugs from Streptomyces cellulosae strain TES17 isolated from rhizosphere of Camellia sinensis. BMC Complementary and Alternative Medicine, 18(1), 82. https://doi.org/10.1186/s12906-018-2154-4
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Zin, N. M., Baba, M. S., Zainal-Abidin, A. H., Latip, J., Mazlan, N. W., & Edrada-Ebel, R. A. (2017). Gancidin W, a potential low-toxicity antimalarial agent isolated from an endophytic Streptomyces SUK10. Drug Design, Development and Therapy, 11, 351–363. https://doi.org/10.2147/DDDT.S121283
Aldulaimi, O., Drijfhout, F., Uche, F. I., Horrocks, P., & Li, W.-W. (2019). Discovery, synthesis and antibacterial evaluation of phenolic compounds from Cylicodiscus gabunensis. BMC Complementary and Alternative Medicine, 19(1), 183. https://doi.org/10.1186/s12906-019-2589-2
Alkandahri, M., Berbudi, A., N, vicahyani utami, & A, S. (2019). Antimalarial activity of extract and fractions of Castanopsis costata (Blume) A.DC. Avicenna J Phytomed., 9(5), 474-481.
Arifiyanto, A., Afriani, H., Putri, M. H., Damayanti, B., & Riyanto, C. L. R. (2021). The biological prospective of red-pigmented bacteria cultured from contaminated agar media. Biodiversitas, Journal of Biological Diversity, 22(3), 1152–1159. https://doi.org/10.13057/biodiv/d220310
Arifiyanto, A., Surtiningsih, T., Ni’matuzahroh, Fatimah, Agustina, D., & Alami, N. (2020). Antimicrobial activity of biosurfactants produced by actinomycetes isolated from rhizosphere of Sidoarjo mud region. Biocatalysis and Agricultural Biotechnology, 24. https://doi.org/10.1016/j.bcab.2020.101513
Cui, L., Mharakurwa, S., Ndiaye, D., Rathod, P. K., & Rosenthal, P. J. (2015). Antimalarial drug resistance: Literature review and activities and findings of the ICEMR network. American Journal of Tropical Medicine and Hygiene, 93(Suppl 3), 57–68. https://doi.org/10.4269/ajtmh.15-0007
Cushnie, T. P. T., Cushnie, B., & Lamb, A. J. (2014). Alkaloids: an overview of their antibacterial, antibiotic-enhancing and antivirulence activities. International Journal of Antimicrobial Agents, 44(5), 377–386. https://doi.org/10.1016/j.ijantimicag.2014.06.001
El-Naggar, N. E. A., & El-Ewasy, S. M. (2017). Bioproduction, characterization, anticancer and antioxidant activities of extracellular melanin pigment produced by newly isolated microbial cell factories Streptomyces glaucescens NEAE-H. Scientific Reports, 7(February), 1–19. https://doi.org/10.1038/srep42129
Ezeonu, C. S., & Ejikeme, C. M. (2016). Qualitative and Quantitative Determination of Phytochemical Contents of Indigenous Nigerian Softwoods. New Journal of Science, 2016, 1–9. https://doi.org/10.1155/2016/5601327
Fang, Q., Maglangit, F., Mugat, M., Urwald, C., Kyeremeh, K., & Deng, H. (2020). Targeted Isolation of Indole Alkaloids from Streptomyces sp. CT37. In Molecules (Vol. 25, Issue 5). https://doi.org/10.3390/molecules25051108
Fatmawaty, Rosmalena, Amalia, A., Syafitri, I., & Prasasty, V. D. (2017). Antimalarial effect of flamboyant (Delonix regia) bark and papaya (Carica papaya L) leaf ethanolic extracts against Plasmodium berghei in mice. Biomedical and Pharmacology Journal, 10(3), 1081–1089. https://doi.org/10.13005/bpj/1206
Fitri, L. E., Alkarimah, A., Cahyono, A. W., Wahyudha, Lady, N., Endharti, A. T., Nugraha, & Rivo Yudhinata Brian. (2019). Effect of Metabolite Extract of Streptomyces hygroscopicus subsp. hygroscopicus on Plasmodium falciparum 3D7 in Vitro. Iran J Parasitol, 14(3), 444–452.
Gomathi, A., & Gothandam, K. M. (2019). Investigation of anti-inflammatory and toxicity effects of mangrove-derived Streptomyces rochei strain VITGAP173. Journal of Cellular Biochemistry, 120(10), 17080–17097. https://doi.org/10.1002/jcb.28969
Hay SI, Guerra CA, Tatem AJ, Noor AM, S. R. (2004). The global distribution and population at risk of malaria: past, present, and future. Lancet Infect Dis., 4(6), 327–336. https://doi.org/10.1016/S1473-3099(04)01043-6
Isaka, M., Jaturapat, A., Kramyu, J., Tanticharoen, M., & Thebtaranonth, Y. (2002). Potent in vitro antimalarial activity of metacycloprodigiosin isolated from Streptomyces spectabilis BCC 4785. Antimicrobial Agents and Chemotherapy, 46(4), 1112–1113. https://doi.org/10.1128/AAC.46.4.1112-1113.2002
Li, C., Ji, C., & Tang, B. (2018). Purification, characterisation and biological activity of melanin from Streptomyces sp. FEMS Microbiology Letters, 365(19), 1–8. https://doi.org/10.1093/femsle/fny077
Lin, Y. B., Wang, X. Y., Fang, H., Ma, Y. N., Tang, J., Tang, M., & Wei, G. H. (2012). Streptomyces shaanxiensis sp. nov., a blue pigment-producing streptomycete from sewage irrigation soil. International Journal of Systematic and Evolutionary Microbiology, 62(8), 1725–1730. https://doi.org/10.1099/ijs.0.029959-0
Notarte, K. I. R., Devanadera, M. K. P., Mayor, A. B. R., Cada, M. C. A., Pecundo, M. H., & Macabeo, A. P. G. (2019). Toxicity , Antibacterial , and Antioxidant Activities of Fungal Endophytes Colletotrichum and Nigrospora spp . Isolated from Uvaria grandiflora. Philippine Journal of Science, 148(September), 505–512.
Nugraha, R. Y., Faratisha, I. F., Mardhiyyah, K., Ariel, D. G., Putri, F. F. N., Winarsih, S., Sardjono, T. W., & Fitri, L. E. (2020). Antimalarial Properties of Isoquinoline Derivative from Streptomyces hygroscopicus subsp. Hygroscopicus: An In Silico Approach. BioMed Research International. https://doi.org/10.1155/2020/6135696
Okokon, J., Antia, B., MOHANAKRISHNAN, D., & SAHAL, D. (2017). Antimalarial and antiplasmodial activity of husk extract and fractions of Zea mays. Pharmaceutical Biology, 55(1), 1394–1400. https://doi.org/10.1080/13880209.2017.1302966
Orabuezea, C. I., Ota, D. A., & Coker, H. A. (2020). Antimalarial potentials of Stemonocoleus micranthus Harms (leguminoseae) stem bark in Plasmodium berghei infected mice. Journal of Traditional and Complementary Medicine, 10(1), 70–78. https://doi.org/10.1016/j.jtcme.2019.03.001
Pereira, D. M., Valentão, P., Pereira, J. A., & Andrade, P. B. (2009). Phenolics: From chemistry to biology. Molecules, 14(6), 2202–2211. https://doi.org/10.3390/molecules14062202
Rani, R., Arora, S., Kaur, J., & Manhas, R. K. (2018). Phenolic compounds as antioxidants and chemopreventive drugs from Streptomyces cellulosae strain TES17 isolated from rhizosphere of Camellia sinensis. BMC Complementary and Alternative Medicine, 18(1), 82. https://doi.org/10.1186/s12906-018-2154-4
Singh B, D. C. (2013). Human infections and detection of Plasmodium knowlesi. Clinical. Microbiology Reviews, 26(2), 165–84.
Zeleke, G., Kebebe, D., Mulisa, E., & Gashe, F. (2017). In Vivo Antimalarial Activity of the Solvent Fractions of Fruit Rind and Root of Carica papaya Linn (Caricaceae) against Plasmodium berghei in Mice. Journal of Parasitology Research. https://doi.org/https://doi.org/10.1155/2017/3121050
Zhou, H., Yang, Y., Zhang, J., Peng, T., Zhao, L., Xu, L., & Ding, Z. (2013). Alkaloids from an endophytic streptomyces sp. YIM66017. Natural Product Communications, 8(10), 1393–1396. https://doi.org/10.1177/1934578x1300801012
Zin, N. M., Baba, M. S., Zainal-Abidin, A. H., Latip, J., Mazlan, N. W., & Edrada-Ebel, R. A. (2017). Gancidin W, a potential low-toxicity antimalarial agent isolated from an endophytic Streptomyces SUK10. Drug Design, Development and Therapy, 11, 351–363. https://doi.org/10.2147/DDDT.S121283
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