Diversity of endophytic bacteria and microfungi in Syzygium cumini fruit from West Java, Indonesia

##plugins.themes.bootstrap3.article.main##

IDA INDRAWATI
NIA ROSSIANA
MUHAMMAD FAIZAL FATHURROHIM

Abstract

Abstract. Rossiana N, Fathurrohim MF, Indrawati I. 2021. Diversity of endophytic bacteria and microfungi in Syzygium cumini fruit from West Java, Indonesia. Biodiversitas 22: 3943-3948. Syzygium cumini L. Skells is a native evergreen tropical tree in Southeast Asia belong to the Myrtaceae family, known as the Java plum, jambul, jambolan, jamblang, or jamun. The bacterial and fungal endophytes associated with fruit have not been determined and functionally characterized. The endophytic microbes live inside the surface-sterilized fruits and have no visibly harmful effects on the plants. The purposes of the study were to isolate, characterize, and determine the diversity of endophytic bacteria and fungi in S. cumini fruit. The endophytes from S. cumini fruit were observed morphologically for identification. The result of isolation and identification showed there are four bacterial isolate endophytes (B. cereus, B. subtilis, B. megaterium, and Bacillus sp.) and four fungal endophytes (Candida guilliermondii, Penicillium sp., Mycelia sterilia, and Aspergillus sp.) isolated from S. cumini fruit.


 


Keywords:Biodiversity, Bacterial endophyte, Fungi endophyte, Jamblang

##plugins.themes.bootstrap3.article.details##

References
Alina SO, Constantinscu F and Petruta CC. 2015. Biodiversity of Bacillus subtilis group and Benefical Traits of
Bacillus species Useful In Plant Protection. Romanian Biotechnological Latters. Vol. 20. No. 5:
– 10750.
Anjum N and Chandra N. 2015. Endophytic Bacteria: Optimization of Isolation Procedure From Various
Medicinal Plants and TheirPreliminary Characterization. Asian Journal of Pharmaceutial and Clinical
Research. Vol. 8. Issue. 4: 233 – 238.
Ariyanto EF, Abadi, AL. and Djauhari. S. 2013. Keanekaragaman Jamur Endofit Pada Daun Tanaman Padi
(Oryza sativa .L) Dengan Sistem Pengelolaan Hama Terpadu (PHT) Dan Konvensional Di Desa
Bayem, Kecamatan Kasembon, Kabupaten Malang. Jurnal HPT. Vol. 1. No. 2: 37 – 51.
Desriani, Ukhradia Maharaniq Safira P, Maria Bintang, Akhmad Rivai, Puspita Lisdiyanti. 2014. Isolasi dan
Karakterisasi Bakteri Endofit dari Tanaman Binahong dan Katepeng China. Jurnal FK Unand. Vol. 3
No. 2: 89 – 93.
Hermanto C, Indrianti. NLP, dan Hardiati S. 2013. Keragaman dan Kekayaan Buah Tropika Nusantara.
Badan Penelitian dan Pengembangan Pertanian. Kementrian Pertanian. IAARD Press. Jakarta.
Gimernia C, Pizzarelli G, Cristini F, Barchiesi F, Sperghini E, Scalise and Martino P. 2006. Candida
guilliermondii Fungemia in Patients with Hematologic Malignancies. Journal of Clinical
Microbiology. Vol. 44. No. 07: 2458 – 2464.
Goryluk A, Burlaga HB and Blaszczyk M. 2009. Isolation and Charecterization of Bacterial Endophytes of
Chelidonium maju L. Polish Journal of Microbiology. Vol. 58. No. 4: 355 – 361.
Marliani L, Kusriani H dan Sari, NI. 2014. Aktivitas Antioksidan Daun dan Buah Jamblang (Sygizium
cumini L.) Skeel. Prosiding SNaPP2014 Sains, Teknologi, dan Kesehatan: 201-206. [Indonesia].
Mobitec. 2008. Bacillus Megaterium Protein Expression System. Molecular Bioteknology. Germany.
Munif A, Johannes H, and Richard S. 2012. Isolation of Endophytic Bacteria from Tomato
and Their Biocontrol Activities against Fungal Disease. Journal Microbiology Indonesia.Vol. 6. No. 4:
– 156.
Nicoletti R and Fiorentino. A. 2015. Plant Bioactive Metabolites and Drugs Produced by Endophytic Fungi of
Spermatophyta. Agriculture: 918 – 970.
Nyongesa BW, Okoth S and Ayugi V. 2015. Identification Key for Aspergillus Species Isolated from Maize
and Soil of Nandi County, Kenya. Advances in Microbiology. Vol. 5: 205 – 229.
Periago PM, ConesaR, Delgado B, Fernandez PS, and Palop A. 2006. Bacillus megaterium Spore
Germination and Growth Inhibition by a Treatment Combining Heat with Natural Antimicrobials.
Food Technol. Biotechnol. No. 44. Vol. 1: 17 – 23.
Petit P, Lucas EMF, Abreu ML, Pfenning LH, and Takahashi JA. 2009. Novel antimicrobial
secondary metabolites from a Penicillium sp. isolated from Brazilian cerrado soil. Electronic Journal of
Biotechnology. Vol.12. No.4: 1 – 9. Public Health England. 2015. Identification of Bacillus Species.
NHS. London.
Saleh F, Kheirandish F, Azizi H, and Azizi M. 2014. Molecular Diagnosis and Characterization of Bacillus
subtilis Isolated From Burn Wound in Iran. Research in Molecular Medicine. Vol. 2. Issue. 2: 40 – 44.
Shekhawat KK, Rao. DV and Batra A. 2010. Morphological Study of Endophytic Fungi Inhabiting Leaves
of Melia azedarach L. International Journal of Pharmaceutical Sciences Review and Research.
Volume 5. Issue 3: Article-032: 117-180.
Subowo YB. 2015. Pengujian aktifitas jamur Penicillium sp. R7.5 dan Aspergillus niger NK pada media
tumbuh untuk mendukung pertumbuhan tanaman padi di lahan salin. Prosiding Seminar Nasional
Masy Biodiversity Indonesia. Vo. 1. No. 5: 1136 – 1141. [Indonesia].
Srinivas RP, Nigam A, Aruna J, Silva WCD, and Chikkaswanny BK. 2015. An Investigation of
Biodiversity of Endophytic Fungi Associated With Some Medical plants. International Journal of
Advanced Research in Engineering and Applied Sciences. Vol. 4. No. 2: 27 – 44.
Sumardi, Ekowati. CN, Handayani K. and Nurhayati. 2012. Isolasi dan Karekteristik Bacillus sp. Penghasil
Antimikroba dari Saluran Penernaan Ayam Kampung (Gallus domesticus). Prosiding SNSMAIP III:
– 311. [Indonesia].
Zhang. HS, et. al. 2016. Two New Secondary Metabolites from the Endophytic Fungus Endomelanconiopsis
endophytica. Molecules. Vol. 21. No. 943: 1 – 6.