Bioprospecting of cow's ruminal microbiota from a slaughterhouse in Ambarawa, Central Java, Indonesia
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Murwani R, Sibero MT, Silitonga PRA, Ambariyanto A. 2021. Bioprospecting of cow's ruminal microbiota from a slaughterhouse in Ambarawa, Central Java, Indonesia. Biodiversitas 22: 5030-5038. Ruminal microorganisms play essential roles in maintaining ruminant health. However, most studies focused only on ruminal lactic acid bacteria (LAB), although other ruminal microorganisms might have biological properties for biotechnological purposes. Therefore, the current study aimed to isolate ruminal bacteria (LAB and non-LAB) and fungi from ruminal material and conducted a bioprospecting study to understand their ability to produce antibacterial compounds and polysaccharide-degrading enzymes. The ruminal bacteria were isolated on MRS and ISP4 agar, while PDA was used to isolate the different fungi. The antibacterial property was tested against multidrug-resistant Escherichia coli and Salmonella enterica ser. Typhi. The ability to produce agarase, alginate-lyase, and carrageenase was screened. Prospective isolates were identified using DNA barcoding approach. Twelve bacteria were isolated using MRS agar, six from ISP4 agar, and four fungi from PDA. Among twelve bacteria from MRS agar, eleven were considered LAB, which consisted of Lactobacillus plantarum and Pediococcus acidilactici. Several classes of bacteria such as actinobacteria, firmicutes, ?-proteobacteria, and ?-proteobacteria were isolated during this study. In addition, three fungal classes, namely Saccharomycetes, Eurotiomycetes, and Mucoromycetes were also isolated. All bacteria from MRS agar were suggested to have potential compounds with antimicrobial activity, while all ruminal fungi exhibited potential sources of polysaccharide-degrading enzymes.
##plugins.themes.bootstrap3.article.details##
Most read articles by the same author(s)
- MADA TRIANDALA SIBERO, ANGGUN PUSPITARINI SISWANTO, RETNO MURWANI, EVAN HANSEL FREDERICK, ALDI PRATAMA WIJAYA, EMMY SYAFITRI, KINDI FARABI, SHUN SAITO, YASUHIRO IGARASHI, Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 9 (2020)
- RHESI KRISTIANA, MADA TRIANDALA SIBERO, MACELLYNE YOHANNA FARISA, DIAH AYUNINGRUM, DIO DIRGANTARA, MUHAMMAD HANAFI, OCKY KARNA RADJASA, AGUS SABDONO, AGUS TRIANTO, Antibacterial potential of nudibranch-associated bacteria from Saparua and Nusa Laut Islands, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 7 (2019)
- MADA TRIANDALA SIBERO, RUDHI PRIBADI, STEFANIE JESSICA HENNY LARASATI, MARK S CALABON, AGUS SABDONO, SUBAGIYO, EVAN HANSEL FREDERICK, Diversity of sponge-associated fungi from a mangrove forest in Kemujan Island, Karimunjawa National Park, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 12 (2021)
- ABDUL HAMID A. TOHA, MUHAMMAD DAILAMI, SAIFUL ANWAR, JUSWONO B. SETIAWAN, YUSUP JENTEWO, IDA LAPADI, SANNY SUTANTO, RATIH ARYASARI, AMBARIYANTO, FERAWATI RUNTUBOI, HAWIS MADDUPPA, The genetic relationships and Indo-Pacific connectivity of whale sharks (Rhincodon typus) with particular reference to mitochondrial COI gene sequences from Cendrawasih Bay, Papua, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 5 (2020)
- ALDI PRATAMA WIJAYA, AGUS SABDONO, MADA TRIANDALA SIBERO, AGUS TRIANTO, OCKY KARNA RADJASA, Antimicrobial activity of nudibranch Chromodoris lineolata associated bacteria against skin diseases pathogens from Jepara Coastal Waters, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 4 (2022)
- RETNO HARTATI, WIDIANINGSIH WIDIANINGSIH, AGUS TRIANTO, MUHAMMAD ZAINURI, AMBARIYANTO AMBARIYANTO, The abundance of prospective natural food for sea cucumber Holothuria atra at Karimunjawa Island waters, Jepara, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 18 No. 3 (2017)
- MADA TRIANDALA SIBERO, EVAN HANSEL FREDERICK, AGUS SABDONO, DIAH PERMATA WIJAYANTI, DELIANIS PRINGGENIES, OCKY KARNA RADJASA, DEWI SESWITA ZILDA, RETNO MURWANI, First report of seaweed-associated yeast from Indonesia: Species composition and screening of their polysaccharides-degrading enzymes , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 3 (2022)
- JIBRIEL FIRMAN SOFYAN, AMBARIYANTO AMBARIYANTO , KEN SUWARTIMAH, ABDUL HAMID A. TOHA, Relationship between the biomass of reef shark and fish in South Morotai Waters, North Maluku, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 12 (2020)
- ADITYA IRAWAN, SUPRIHARYONO SUPRIHARYONO, JOHANNES HUTABARAT, AMBARIYANTO AMBARIYANTO, Seagrass beds as the buffer zone for fish biodiversity in coastal water of Bontang City, East Kalimantan, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 19 No. 3 (2018)