Short Communication: The potential of Sulfate Reducing Bacteria of ex-coal mine sediment pond as sulfate reducing agents of acid land in Samarinda, Indonesia

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DOI https://doi.org/10.13057/bonorowo/w070204
Issue
Vol. 7 No. 2 (2017)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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EKO KUSUMAWATI ♥
Laboratory of Microbiology and Molecular Genetics, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman. Samarinda 75123, East Kalimantan, Indonesia
SUDRAJAT SUDRAJAT
Laboratory of Animal Anatomy and Microtechnique, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman. Samarinda 75123, East Kalimantan, Indonesia
IKA PURNAMASARI
Laboratory of Economics and Business, Department of Mathematics, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman. Samarinda 75123, East Kalimantan, Indonesia
BINA CRISTYANTI PANGGABEAN
Laboratory of Microbiology and Molecular Genetics, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman. Samarinda 75123, East Kalimantan, Indonesia
MAIDA APRIYANTI
Laboratory of Microbiology and Molecular Genetics, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman. Samarinda 75123, East Kalimantan, Indonesia

Abstract

Abstract. Kusumawati E, Sudrajat, Purnamasari I, Panggabean BC, Apriyanti M. 2017. Short Communication: The potential of Sulfate Reducing Bacteria of ex-coal mine sediment pond as sulfate-reducing agents of acid land in Samarinda, Indonesia. Bonorowo Wetlands 7: 79-82. The study aims to determine the effect of pH medium on the growth of sulfate-reducing bacteria taken from the e-coal mine sediment pond and determine its potential as a reducing sulfate agent of acid ex-coal mine land in Samarinda East Kalimantan, Indonesia. This study used six SRB isolated from an ex-coal mine sediment pond in Samarinda. The SRB potency test in reducing sulfate was conducted by growing the SRB on Postgate liquid medium at different pHs of 2, 4, and 6 by adding acid soils on each treatment. The results showed that sulfate reducing bacteria isolated from ex-coal mine sediment pond in Samarinda, i.e., sp.1 (Desulfococcus sp.), sp.2 (Desulfotomaculum sp.), sp.3 (Desulfobacter sp.), sp.4 (Desulfobulbus sp.), sp.5 (Desulfobacterium sp.) and sp.6 (Desulfotomaculum sp.) had potential as sulfate reducing agent of acid land. The efficiency of sulfate reduction was 89%, 91%, and 91% in the pH of 2, 4, and 6, respectively. This indicated that the highest sulfate reduction is in the medium with pH 4 and 6. In addition, sp.5 (Desulfobacterium sp.) growing on medium at pH 4 had the best sulfate reduction efficiency (93%) compared with other SRB isolates.

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References
Atlas RM, Bartha R. 1992. Hydrocarbon biodegradation and oil spill
bioremediation. In: Marshall KC (ed.). Microbial Ecology. Vol. 12.
Plenum Press. New York.

Djurle C. 2004. Development of Model for Simulation of Biological
Sulphate Reduction with Hidrogen as Energy Source. [Thesis].
Department of Chemical Engineering, Lund Institute of Technology,
The Netherlands

Doshi SM. 2006. Bioremediation of Acid Rock Drainage Using Sulfate
Reducing Bacteria. U.S. Environmental Protection Agency Office of
Solid Waste and Emergency Response Office of Superfund
Remediation and Technology Innovation, Washington, D.C.

Fahruddin. 2010. Bioteknologi Lingkungan. Penerbit Alfabeta, Bandung.
[Indonesian]

Frank F. 2000. Bioremediation by sulfate reducing bacteria of acid mine
drainage. Department of Environmental Sciences,UC Berkeley, CA,
USA.

Groudev SN, Komnitsas K, Spasova II, Paspaliaris I. 2001. Treatment of
AMD by a natural wetland. Minerals Eng 12: 261-270.

Hards BC, Higgins JP. 2004. Bioremediation of Acid Rock Drainage
Using SRB. Jacques Whit Environment Limited, Ontario.

Havlin JL, Beaton JD, Tisdale SL, Nelson WL. 1999. Soil Fertility and
Fertilizers An Introduction to Nutrient Management. 6 th ed. Prentice
Hall, Upper Saddle River, New Jersey.

Lieberg EW, Cutright TJ. 1999. The investigation of enhanced
biormediation through the addition of macro dan micro nutrisis in
PAHs contaminated soil. J Intl Biodet Biodegrad 44: 55-64.

Tala’ohu SH, Moersidi S, Sukristiyonubowo, Gunawan S. 1995. Sifat
fisikokimia tanah timbunan batubara (PT BA) di Tanjung Enim,
Sumatera Selatan. Dalam Prosiding Pertemuan Pembahasan dan
Komunikasi Hasil Penelitian Tanah dan Agroklimat. Buku IV. Bidang
Konservasi Tanah dan Air serta Agroklimat. Puslitbangtanak.
Cisarua, 26-28 September 1995. [Indonesian]

van Houten RT, Hulshoff Pol LW, Lettinga G. 1994. Biological sulphate
reduction using gas-lift reactors fed with hydrogen and carbon
dioxide as energy and carbon source. Biotechnol Bioeng 44: 586-594.

van Houten RT, van der Spoel H, van Aelst AC, Hulshoff Pol LW,
Lettinga G. 1995b. Biological Sulfate Reduction Using Synthesis Gas
as Energy and Carbon Source. Biotechnology and Bioengineering 50:
136-144.

Widyati E. 2006. Bioremediasi Tanah Pasca tambang Batubara dengan
Sludge Industri Kertas untuk Memacu Revegetasi Lahan. [Disertasi].
Sekolah Pascasarjana IPB. Bogor. [Indonesian]

Widyati E. 2007. Pemanfaatna bakteri pereduksi sulfat untuk bioremediasi
tanah bekas tambang batubara. Biodiversitas 8 (4): 283-286.
[Indonesian]