Goramy spermatozoa quality after sub-zero freezing: The role of coconut water as the cryoprotectant

Abstract

Abstract. Abinawanto, Putri PE. 2017. Goramy spermatozoa quality after sub-zero freezing: The role of coconut water as the cryoprotectant. Cell Biol Dev 1: 1-5. The coconut water effect combined with 5% of glycerol for preserving goramy spermatozoa at -34 °C for 48 hours has been studied. The study's objective is to find the best combination among concentrations of coconut water on 0%, 21%, 23%, 25%, 27%, and 29%, respectively, combined with 5% of glycerol for maintaining good spermatozoa motility, viability and minimizing spermatozoa abnormality. One part of semen/sperm was mixed with three parts of solvent (5% glycerol + fish ringer + coconut water) and equilibrated at 4°C for 45 min. The diluted sperm were then frozen at -34 °C for 48 h. Finally, cryopreserved sperms were thawed at 30 °C for 3-5 min. Spermatozoa quality was evaluated before and after sub-zero freezing. Based on the Kruskal-Wallis test, spermatozoa motility and viability were higher than control (P<0.05), while the spermatozoa abnormality was not significantly different compared to control (P>0.05). Twenty-five percent of coconut water combined with 5% of glycerol was the best combination for preserving spermatozoa motility (80.36±1.54)% and spermatozoa viability (82±1.86)%, and also minimized spermatozoa abnormality (10±1.03)%.