The antifungal effect of ethanolic extract of Aloe vera leaves on the growth of Trichophyton rubrum in vitro

Abstract

Abstract. Salim FS, Djumarga S, Sari Y. 2019. The antifungal effect of ethanolic extract of Aloe vera leaves on the growth of Trichophyton rubrum in vitro. Biofarmasi J Nat Prod Biochem 17: 81-85. This study aims to determine if there is an antifungal effect of the ethanolic extract of Aloe vera L. leaves on the growth of Trichophyton rubrum in vitro. This study is a quasi-experimental study with a posttest-only-control-group design. Pure T. rubrum cultures were obtained from the Microbiology Laboratory, Universitas Setia Budi, Surakarta, Central Java, Indonesia. Sampling was inoculated by simple random sampling with the criteria of a 2-day-old mushroom. Colonies of T. rubrum on Sabouraud Dextrose Agar were taken and then diluted with 0.9% NaCl until it reached a turbidity equivalent to 0.5 Mc Farland standard, then smeared evenly on a petri dish containing Sabouraud agar. Samples were divided into 6 groups: negative control in distilled water, extracts with concentrations of 10%, 20%, 40%, 80%, and 100%. Each group consisted of 5 wells. All petri dishes were incubated for 4 days at room temperature. Data in the form of the diameter of the inhibition zone were analyzed using the Kruskal-Wallis test and then continued with post hoc Mann-Whitney analysis. The results showed at least an antifungal effect on the ethanolic extract of A. vera leaves on the growth of T. rubrum in vitro between the two groups. There were significant differences between the treatment groups except in the concentration group of 10% with 20% and the concentration group of 20% with 40%. There is an antifungal effect on the ethanolic extract of A. vera leaves on the growth of T. rubrum in vitro, with a concentration of 100% having sensitive results, 80% concentration having intermediate results, and concentrations of 10%, 20%, and 40% having positive results.