The activity test of synbiotic between extract of noni leaf (Morinda citrifolia) and lactic acid bacteria against pathogenic bacteria in broiler chicken in vitro

Abstract

Abstract. Zen A, Purwoko T, Julendra H. 2013. The activity test of synbiotic between extract of noni leaf (Morinda citrifolia) and lactic acid bacteria against pathogenic bacteria in broiler chicken in vitro. Biofarmasi 11: 19-23. The breeders of broiler chicken usually provide the commercial rations to increase the business of chicken livestock because the commercial rations have met the standard needs of the substances of feed that been appointed. The commercial rations that often used as broiler feed are currently using a low-dose antibiotic that will give a harmful effect on human health in the long-term if it continues to be consumed. Therefore, it needs to use bioaditive to replace the antibiotic. One bioadditive is a synbiosis between probiotic and phytophotic that it is expected to inhibit the growth of pathogenic bacteria in poultry. This study was conducted to determine the presence of inhibition of noni leaf extract against BAL and the pathogenic bacteria, and to determine the effect of synbiosis between the noni leaf extract and BAL against the pathogenic bacteria in vitro. This study included the selection of noni leaf extract based on the highest yield and the water content measurement. Thereafter, an antibacterial test was performed by using the selected leaf extract, the neutral supernatant from Pseudococcus acidilactici as BAL, and the synbiotics of both against Escherichia coli, Staphylococcus aureus and Salmonella pullorum bacteria by using the Kirby-Bauer diffusion method. From the results, it was selected the noni leaf extract by ethanol 40% which had a yield value of 20.8% with the water content of 18.9%. The selected noni leaf extract was then tested on BAL and the pathogenic bacteria. The results of antibacterial test showed the smallest inhibition on P. acidilactici occurred in the noni leaf extract with the concentration of 250 mg/mL, while the highest inhibition of noni leaf extract to E. coli was 5.42 mm, S. aureus 3.76 mm and S. pullorum 3.45 mm. The inhibition after the synbiotic against E. coli was 4.75 mm, S. aureus was 3.83 mm, while S. pullorum was 3.58 mm.