The antifungal effect of essential oil of Alpinia galanga rhizome on Microsporum gypseum in vitro

Abstract

Abstract. Indrasari A, Dharmawan R, Djumarga S. 2013. The antifungal effect of essential oil of Alpinia galanga rhizome on Microsporum gypseum in vitro. Biofarmasi 11: 24-30. This study aimed to determine the effect of essential oil of ginger rhizome on Microsporum gypseum in vitro. The study was performed as an experimental kuasi laboratory. The object of study was M. gypseum which took by a purposive sampling standardized by McFarland technique (equivalent with 0.5 McFarland turbidity). The study used the colonies of M. gypseum on 21 Sabouraud Dextrose Agar plates which containing cloramphenikol. Each plate had two or four holes. Every hole was filled by etanol 70% as a negative control (K1), miconazole 5 mg as a positive control (K2) and various concentrations of ginger rhizome extract by K3-K10 (0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5% and 4%). The plates were incubated on the temperature of 30^oC in an incubator for 6 days and measured the diameter of inhibition zone. The data were collected and analyzed by Kruskal-Wallis Test and Mann-Whitney Test by the program of SPSS 16.0 for Windows. The results of study showed that the means of the diameter of inhibition zone (K1) was 6 mm, K2 = 24.4 mm, K3 = 27.2 mm, K4 = 23.5 mm, K5 = 25.3 mm, K6 = 22.7 mm, K7 = 23.1 mm, K8 = 21.14 mm, K9 = 21.14 mm and K10 = 19 mm. The result of Kruskal-Wallis Test showed that there was the difference of the diameter of inhibition zone means between all of group (K1-K10) significantly (p<0.05). The result of Mann-Whitney Test showed that there was the difference between a negative control and all of various concentrations of ginger rhizome extract (p<0.05). The study was concluded that there was an antifungal effect of essential oil of ginger rhizome on M. gypseum in vitro.