An analgetic activity of sedges (Cyperus rotundus L.) extract on white male mice (Mus musculus L.)

Abstract

Abstract. The objectives of the research were to find out the effect of giving sedges root extract orally on the number of writhing after chemical pain induction and time reaction after thermal pain induction of mice and also to find out the extract dosage which influenced a decreasing number of writhing after chemical pain induction and length of reaction time after thermal pain induction of mice. The Complete Random Design (CRD) with 6 treatment groups and each treatment used 5 repetitions were used in this study. The groups were: Group I , control group, treated with sedges root extract of 0 mg/ 20 g BW , 0,5 ml; Group II treated with sedges root extract of 1 mg/ 20 g BW, 0,5 ml; Group III treated with sedges root extract of 3 mg/ 20 g BW, 0,5 ml; Group IV treated with sedges root extract of 5 mg/ 20 g BW, 0,5 ml; Group V treated with sedges root extract of 7 mg/ 20 g BW, 0,5 ml; Group VI treated with asetosal 200 mg/ kg BW , 0,5 ml/ 20 g BW and for the activity test, the sedges root extract was suspended in CMC 1%. Induction of chemical pain was done according to Witkin et al. (1965) in Pudjiastuti et al. (2000), in which 0,1 ml 3% of Acetic Acid/ 20 g BB was injected intraperitoneally 30 minutes after giving the oral-material test. The mouse gave a response in the way of writhing. Thermal pain induction was done by placing the mouse on a hot plate with a constant temperature of 55^oC. The mouse responded by licking its back foot or even jumping. The data collected was analyzed using one direction ANOVA model, and it was continued with the LSD test to find out the difference in every treatment group. The analysis showed that the sedges root extract dosage of 7 mg/ 20 g BB decreased the number of writhing after chemical pain induction and length of mouse time reaction after thermal pain induction. The sedges root extract dosage was 7 mg/ 20 g BB had an analgetic function.