Selection and identification of cellulolitic and lignocellulolitic fungi from organic waste of cajuput oil (Melaleuca leucadendron L.) from KPH Gundih, Regency of Grobogan

Abstract

Abstract. The aims of this research were to isolate and select fungi degrading cellulose and lignocellulose of organic waste from cajuput oil distillation. The research outline is organic waste from cajuput oil distillation containing cellulose and lignocellulose that cause naturally composting process takes a long time. The difficulty of organic waste decomposition depends on the matter in the tissue. To speed up the decomposition process, it needs microorganisms. Fungi have more ability to degrade cellulose and lignocellulose than bacteria. Because of that, it was necessary to isolate and select fungi that could degrade cellulose and lignocellulose of organic waste from cajuput oil distillation. This research isolated and identified fungi from organic waste from cajuput oil distillation and soil where organic waste from cajuput oil distillation was thrown away. The selection of cellulolytic fungi by inoculating fungi on CMC agar medium and selecting lignocellulolytic fungi by inoculating soil dilution 10-4 to agar medium, added to organic waste from cajuput oil distillation. The result showed that a total of 6 fungi was isolated from organic waste from cajuput oil distillation and soil, where organic waste from cajuput oil distillation was thrown away. Aspergillus parasiticus, A. nidulans, and Trichoderma harzianum can degrade cellulose. Fungi that can degrade lignocellulose of organic waste from cajuput oil distillation is A. parasiticus. A. parasiticus potentially degrade cellulose and lignocellulose organic waste from cajuput oil distillation than other fungi found.