The effect of varied NAA concentration agains growth and flavonoid content of Gynura procumbens (Lour) Merr. callus

Abstract

Abstract. The aim of this research is to study the influence of naphthalene acetic acid (NAA) concentration variation in the callus growth and flavonoid content of Daun dewa [Gynura procumbens (Lour) Merr.]. The research framework relied on the benefit of daun dewa as an anti-cancer drug. This matter is enabled by its compound cytotoxic from compound flavonoid contained. Culture in vitro technique serves as the medium to produce the secondary metabolism. The addition of NAA as a treatment for the induction of callus will promote cell enlargement and the growth of the callus so that callus which later will be analyzed by its flavonoid content. This research was conducted in two-phase. The first phase represented the phase of antecedent attempt in the form of callus induction to get the good callus. The addition of NAA and kinetin was conducted to promote cell proliferation. The parameter of this phase is texture, color, and moment of callus appearance. The second phase is the form of NAA concentration variation treatment phase. At this research, it conducted with the random device complete with one factor that is 5 concentration NAA is 0 mg/L; 0,5 mg/L; 1,0 mg/L; 1,5 mg/L and 2,0 mg/L. The parameter of this phase is the callus's wet weight, the callus's dry weight, the callus's texture, the color of the callus, and flavonoid content. This research indicated that the addition of NAA does not influence callus growth and the flavonoid content of callus daun dewa.