Isolation and identification of flavonoid compounds from Curcuma’s rhizome (Curcuma aeruginosa Roxb.)

Abstract

Abstract. This research was aimed to isolate and identify the flavonoid compounds from the Curcuma rhizome (Curcuma aeruginosa Roxb: Zingiberaceae). The extraction was carried out by the Soxhlet method using petroleum ether, chloroform, n-butanol, and methanol as the solvent agent. Those extracts were then qualitatively tested to identify the presence of flavonoids. Flavonoid is then isolated from the extract of petroleum ether by column chromatography. The fraction resulting from chromatographic was analyzed by the thin-layer chromatography (TLC) method. Flavonoid identification was tested by color, spectrophotometer UV-Vis, IR, and GC-MS. The color test result showed that extract of petroleum ether, chloroform, and n-butanol contain flavonoids. The analyzed single fraction from column chromatography showed that f2 contains isoflavone with 2 methoxy and 1 ethyl substitutes, f4 contains isoflavone with 2 methoxy substitutes, then f9 contains isoflavone with 1 hydroxy and 2 methoxy substitutes.