Isolation and characterization of mannanase, xylanase, and cellulase from marine bacteria Bacillus sp.

Abstract

Abstract. Yopi, Djohan AC, Rahmani N, Jannah AM. 2017. Isolation and characterization of mannanase, xylanase, and cellulase from marine bacteria Bacillus sp. Biofarmasi J Nat Prod Biochem 15: 15-20. Isolation, identification, and characterization of mannanase, xylanase, and cellulase-producing indigenous marine bacteria have been conducted from a total of 20 isolates. Based on 16S rDNA sequence analysis, three potential isolates are obtained and identified Bacillus subtilis (M8), Bacillus tequilensis (X4), and Bacillus cereus (C9). The potential strains M8, X4 and C9 can produce mannanase, xylanase and cellulase activities such as 9.5 U/mL; 0.36U/mL;0.56U/mL with optimum pH and temperature 6.0;50^oC, 5.5;70^oC and 8;50^oC, respectively. Based on the TLC analysis, mannanase from M8 and xylanase from X4 has the potential to hydrolyze mannan and xylan for producing oligosaccharides with the size around tri-hexasaccharide as the main product.