Diuretic effect of milk coffee on white rat (Rattus norvegicus) with variation of milk types

Abstract

Abstract. Bistani DA, Listyawati S, Setyawan AD. 2007. The diuretic effect of milk coffee on white rats (Rattus norvegicus) with various milk kinds. Biofarmasi 5: 8-15. The caffeine content in coffee is a mild diuretic that increases glomerule filtration and reduces natrium reabsorption in the renal tubule. Some people mix milk into the coffee because they do not like the bitter taste of caffeine. Milk contains glucose which can cause osmotic diuretic and increase urine excretion. This research aimed to determine the diuretic effects of oral intakes of coffee milk on white male rats (Rattus norvegicus) with various milk kinds. This research was done in Biology Sub Laboratory, Central Laboratory of MIPA UNS, Surakarta, Central Java. A Completely Randomized Design with five groups and four replications to each group was used in this study. The treatments applied for those groups were: (i) aqua dest (group I), (ii) coffee solution (group II), (iii) coffee + sweetened condensed milk solution (group III), (iv) coffee + soymilk solution (group IV), and (v) coffee + skim milk solution (group V). The parameters used for the physical characteristics of urine were volume, color, clearness, pH value, and density. The parameters used for the chemical elements were the glucose analysis by Benedict test for qualitative and spectrophotometry for quantitative, and the analysis of NaCl content by Fantus method. The data were analyzed using variance (ANOVA) and continued with Duncan Multiple Range Test (DMRT) at a significance level of 5%. The results showed that various milk kinds did not affect volume, color, clearness, pH value, density, and glucose content after 4 hours of treatments. Still, NaCl content was affected after 4 hours of treatments.