The growth and reserpine content of callus culture of Rauvolfia verticillata on the variation of sucrose concentration in MS medium

Abstract

Abstract. Irmawati, Solichatun, Anggarwulan E. 2007. The growth and reserpine content of callus culture of Rauvolfia verticillata on the variation of sucrose concentration in MS medium. Biofarmasi 5: 38-46. The aim of this research was to study the effect of the variation of sucrose concentration on the growth and reserpine content on callus culture of Rauvolfia verticillata (Lour.) Baillon. The research was conducted with the callus culture method consisting of two stages. The first stage was callus initiation to induce callus from leaf explant of R. verticillata, and the second stage was the reserpine production on the treatment medium. This research used a Completely Randomized Design (CRD) by one factor, i.e., the variation of sucrose concentration. The sucrose concentration consisted of five levels, i.e., 0 g/L, 10 g/L, 20 g/L, 30 g/L, and 40 g/L, each concentration in five replicates. The collected data consisted of qualitative and quantitative data. The qualitative data, callus morphology, were presented descriptively. The quantitative data, including fresh weight callus, dry weight callus, and reserpine content, were analyzed using ANOVA and followed by DMRT at a 5% significance level. The result of the research showed that the variation of sucrose concentration influenced fresh weight callus, dry weight callus, and reserpine content. The increase of sucrose concentration tended to raise callus growth, which could be seen from the fresh and dry weight callus. The highest fresh weight callus was found in medium with a sucrose concentration of 20 g/L, while the highest dry weight callus was found in medium with a sucrose concentration of 40 g/L. Increasing sucrose concentration until 30 g/L raised reserpine content, but the sucrose concentration over 30 g/L decreased the reserpine content.