The effect of ethanolic and petroleum ether fractions of bawang dayak (Eleutherine palmifolia) bulb extract on expression of p53 mutant in breast cancer cell line T47D

Abstract

Sudarmawan IH, Dlilir D, Mudigdo A, Budiani DR. 2010. The effect of ethanolic and petroleum ether fractions of bawang dayak (Eleutherine palmifolia) bulb extract on expression of p53 mutant in breast cancer cell line T47D. Biofarmasi 8: 17-26. Breast cancer was still to be the most popular disease. The second highest morbidity and mortality stages after cervix cancer which need to be involved in alternative therapy. Bawang dayak (Eleutherine palmifolia (L.) Merr ) had been used as anti-cancer in empiric therapy by urban society. Therefore, it was needed to determine the influence of etanolic and petroleum eter fractions of bawang dayak extract on the p53 mutant expression in T47D breast cancer cell in vitro. Epithelial cell in ductal mammae breast cancer T47D which had a malignancy and p53 mutant, with ER/PR positive status. As a comparation, it was used MCF7 cell specimen (negative control). They were cultured on 60 wells for T47D and MCF7 in RPMI 1640 media. Each well was filled with 2x105 cells/200 µl media. Thirty wells with a size of 1.5 cm/T47D cell diameter, cultured in etanolic and petroleum eter fractions with under concentration of LC50. The number of sample was determined by using “Rule of Thumbâ€. The result of LC50 on T47D breast cancer cell were etanolic fraction 125 Î¼g/mL, while petroleum eter fraction 31.25 μg/mL. The result of expression percentage of p53 mutant given by the extract of etanolic fraction of bawang dayak were: 0 μg/mL = 36.11%, 15.625 μg/mL = 28.32%, 31.25 μg/mL = 27.46%, 62.5 μg/mL = 19.67%, and 125 Î¼g/mL = 11.02%. Other result of bawang dayak extract percentage given in petroleum eter fraction were: 0 μg/mL = 26.16%, 3.90625 Î¼g/mL = 25.29%, 7.8125 μg/mL = 22.70%, 15.625 μg/mL = 22.27%, and 31.25 μg/mL =15.78%. The treatment of bawang dayak extract in ethanolic and petroleum eter fractions were able to inhibit the expression of p53 mutant in vitro. The result of this research showed no significant difference in the inhibition expression of p53 mutant in breast cancer cell T47D.