The effect of meniran (Phyllanthus niruri) extract protection on mice gastric histology damage induced by aspirin

Abstract

Rachmawati P, Suparyanti EL, Isdariyanto. 2010. The effect of meniran (Phyllanthus niruri) extract protection on mice gastric histology damage induced by aspirin. Biofarmasi 8: 41-46. This experiment aimed to determine the effect of meniran (Phyllanthus niruri Linn.) extract protection on mice (Mus musculus) gastric histology damage induced by aspirin. This study belongs to an experimental laboratory research with post-test only controlled group design. The tested animals used were 25 Swiss Webster male mice in 6-8 weeks age and ±20 g body weight, divided into 5 groups: (i) control group (K) that given with 0.2 mL CMC, Na 0.5% and 0.1 mL aquadest, (ii) treatment group I (P1) that given with 0.2 mL aquadest and 0.1 mL aspirin with a dosage of 1.7 mg/20 g BW mice per oral, (iii) treatment group II (PII) that given with 0.2 mL meniran extract with a dosage of 1.3 mg/20 g BW per oral and 0.1 mL aspirin with a dosage of 1.7 mg/20 g BW mice per oral, (iv) treatment group III (PIII) that given with 0.2 mL meniran extract with a dosage of 2.6 mg/20 g BW per oral and 0.1 mL aspirin with a dosage of 1.7 mg/20 g BW mice per oral, and (v) a positive control group (PIV) that given with 0.2 Cimetidine with a dosage of 2.6 mg/20 g BW per oral and 0.1 mL aspirin with a dosage of 1.7 mg/20 g BW mice per oral. The gastric histological description could be seen with a bright light microscope with 100x magnification followed by 400x magnification. Then, the gastric histological description was divided into normal, light damage, and heavy damage. The data obtained were analyzed by using Kruskal Wallis and Mann Whitney statistical tests at α = 0.05. The results of Kruskal Wallis statistical test showed that there was at least one population with a value that higher than other population. The result of Mann Whitney statistical test showed that there was a significant difference between groups K-PI, PI-PII, PI-PIII, and PI-PIV, and no significant difference between K-PII, K-PIII, K-PIV, PII-PIII, PII-PIV, and PIII-PIV. The conclusion of the research was the meniran (Phyllanthus niruri Linn.) extract can give a protection on mice (Mus musculus) gastric histological damage induced by aspirin.