An examination of various concentrations of mahkota dewa, meniran extract, and liquid organic fertilizer in Centella asiatica shoot growth in vitro

Abstract

Abstract. Siskayanti V, Arniputri RB, Praswanto. 2016. An examination of various concentrations of mahkota dewa, meniran extract, and liquid organic fertilizer in Centella asiatica shoot growth in vitro. Biofarmasi 14: 47-55. Centella (Centella asiatica L) is a plant utilized in traditional medicine because it has many benefits, one of which is to fulfill the brain requirement. This research aimed to examine Centella asiatica shoot growth in vitro on the various media types. The concentrations of meniran, mahkota dewa, and liquid organic fertilizer used by tissue culture and filtering (2k design). This method could be screened for the primary media types, organic material types (mahkota dewa and meniran extract), and the expected concentrations (meniran, mahkota dewa, and liquid organic fertilizer). This research was taken place in the Plant Physiology and Biotechnology Laboratory of Agriculture Faculty of Sebelas Maret University from November 2010 to June 2011. This research used a 2k design with a RAL environment. Factor 1: Media type (½ MS and MS); Factor 2: Concentration of liquid organic fertilizer (2 mL/L and 20 mL/L); Factor 3: Concentration of meniran 5 mL/L and 25 mL/L); and Factor 4: Concentration of mahkota dewa (5 mL/L and 25 mL/L). Thus, there are 24 = 16 treatment combinations, each of which is repeated three times. The variables observed are shoot emerging duration, shoot number, shoot height, and leaves a number. The data were analyzed descriptively. The research showed that basic media MS, that basic media MS, POC 20 mL/L, and meniran extract 5 mL/L provided the best explants growth of Centella through in vitro.