The difference of caffeic acid phenethyl ester level in ethanol extract propolis and water extract propolis
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Abstract
Hastuti RD, Prasetyo D, Hadinoto SH. 2013. The difference of caffeic acid phenethyl ester level in ethanol extract propolis and water extract propolis. Biofarmasi 11: 43-47. Propolis is a natural product derived from plant resin collected by honeybeen and its composition has wide range of biological activity. CAPE is the ester of caffeic acid, a derivative of phenolic acid, a flavonoid-like compound, one of the major components of propolis. CAPE is reported can protect nephrotoxicity due to cisplatin induction, inhibiting the growth of various types of abnormal cells, having an anti-inflammation activity, antioxidant activity, cyclooxygenase activity, vasodilatation activity in rat aorta, potential inhibitor of nitric oxidant, inhibitor oxidative process, and effectively suppress the growth of cataract in rat. Propolis can not be used as raw material, and it must be purified by extraction with solvent. Different solvents may extract different compounds, influencing its activity. The purpose of this research was to determine the difference levels of CAPE in ethanol extract propolis and water extract propolis. This research was a pure experimental research using the post-test only design. The sample of this research was raw propolis taken from bees farming in Gejen RT. 03 RW. 02, Kerjo, Karanganyar. Sampling was performed by a purposive sampling. Sample was extracted with a maserasion method with the solvents of ethanol and water. In each extract propolis was made five samples. The determination of the level of CAPE on propolis was performed by using UV-Vis spectrophotometry with Prussian Blue method. The data of the level of CAPE was analyzed by the statistical test of independent t-test. The results showed that the average of CAPE level in ethanol extract propolis was 12.268±0.658 μg/mL and the average of CAPE level in water extract propolis was 5.564±0.332 μg/mL, with p=0.125 (p>0.05). There was no significant difference between extract ethanol propolis and water extract propolis.