The inhibiting effect of Sumbawa honey bee to the growth of Staphylococcus aureus isolated from surgical wound infection

Abstract

Zulhawa DJ, Maryani, Dewi NH. 2014. The inhibiting effect of Sumbawa honey bee to the growth of Staphylococcus aureus isolated from surgical wound infection. Biofarmasi 12: 40-44. Sumbawa Honey contains a high sugar, acid pH, inhibine, glycosides, polyphenols, and H2O2, which could inhibit bacterial growth. This study aimed to determine whether there Sumbawa Honey inhibition effect to the growth of Staphylococcus aureus isolated from surgical wound infection of Amal Sehat Hospital Sragen. This research is laboratory research using post test design only. The sampling technique used was quota sampling. The subject of this research are S. aureus isolated from surgical wound infection of Amal Sehat Islamic Hospital Sragen. The method used is pitting diffusion method. The pit on Muller Hinton agar was made using tools that created pit 6 mm in diameter. S. aureus was obtained standardized with 0.5 Mc Farland standard and then applied using a sterile cotton stick on Muller Hinton media and each of pit filled with aquabides as a negative control, amikacin disc as a positive control, Sumbawa Honey with the concentration of 20%, 40%, 60%, 80%, 100%. Incubated for 18- 24 hours, then inhibition zone formed was measured. The data obtained were processed with the Statistical Product and Service Solution (SPSS). 15.0 for windows. Data analysis using One-Sample Test and T-Test. The results showed a significant inhibiting power of Sumbawa Honey on the growth of S. aureus clinical isolates and standard isolates. However, these effects do not have a statistically significant difference. As the conclusion, Sumbawa Honey has an inhibiting power at a concentration of 20%, 40%, 60%, 80%, 100% on the growth of S. aureus bacteria.