Evaluation of the antiproliferative activity of red propolis hydroalcoholic extract and its fractions obtained by partition

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DOI https://doi.org/10.13057/biofar/f180203
Issue
Vol. 18 No. 2 (2020)
Section
Articles

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IARA SILVA SQUARISI
Universidade de Franca. Avenida Dr. Armando Salles de Oliveira, 201, 14404-600, Franca, São Paulo, Brazil
KAROLINE SOARES DE FREITAS
Universidade de Franca. Avenida Dr. Armando Salles de Oliveira, 201, 14404-600, Franca, São Paulo, Brazil
DANIELI CRISTINA LEMES
Universidade de Franca. Avenida Dr. Armando Salles de Oliveira, 201, 14404-600, Franca, São Paulo, Brazil
GARI VIDAL CCANA-CCAPATINTA
School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo. Av. do Café, s/n, 14040-903, Ribeirão Preto, São Paulo, Brazil
JENNYFER ANDREA ALDANA MEJÍA
School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo. Av. do Café, s/n, 14040-903, Ribeirão Preto, São Paulo, Brazil
JAIRO KENUPP BASTOS
School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo. Av. do Café, s/n, 14040-903, Ribeirão Preto, São Paulo, Brazil
RODRIGO CASSIO SOLA VENEZIANI
Universidade de Franca. Avenida Dr. Armando Salles de Oliveira, 201, 14404-600, Franca, São Paulo, Brazil
SERGIO RICARDO AMBROSIO
Universidade de Franca. Avenida Dr. Armando Salles de Oliveira, 201, 14404-600, Franca, São Paulo, Brazil
DENISE CRISPIM TAVARES ♥
Universidade de Franca. Avenida Dr. Armando Salles de Oliveira, 201, 14404-600, Franca, São Paulo, Brazil

Abstract

Abstract. Squarisi IS, De Freitas KS, Lemes DC, Ccana-Ccapatinta GV, Mejia JAA, Bastos JK, Veneziani RCS, Ambrosio SR, Tavares DC. 2020. Evaluation of the antiproliferative activity of red propolis hydroalcoholic extract and its fractions obtained by partition. Biofarmasi J Nat Prod Biochem 18: 70-73. The present study aimed to evaluate the cytotoxicity of red propolis hydroalcoholic extract (RPHE) and its fractions obtained by partition, hexanes (HF), dichloromethane (DF), ethyl acetate (AF), and n-butanol (BF) on tumor and non-tumor cell lines. For this purpose, the XTT colorimetric assay was performed on human lung fibroblasts (GM07492A, non-tumor cell), breast adenocarcinoma (MCF-7), glioblastoma (U343), and cervix adenocarcinoma (HeLa) cells. The results showed that RPHE, HF, and DF presented not only cytotoxic potential to all tumor cell lines but also to normal cell lines, indicating selectivity absence. HF presented the lowest IC50 (half minimal inhibitory concentration; 33.8-133.3 µg/mL), with a significant difference from those observed for RPHE (137.0-262.7 µg/mL). BF and AF revealed an IC50 higher than 1250 µg/mL in all cell lines. The results showed that red propolis has substances with antiproliferative activity, indicating that its hexane fraction may have substances with antitumor potential.

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