Comparative wound healing potential of Mitracarpus hirtus ointment and honey in diabetic albino rats by collagen assessment
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BAWA INALEGWU
JACOB AONDONGUSHA JATO
♥
https://orcid.org/0000-0003-3011-6605
OVYE AKYENGO
JOHN AKIGHIR
Abstract
Abstract. Inalegwu B, Jato JA, Akyengo O, Akighir J. 2021. Comparative wound healing potential of Mitracarpus hirtus ointment and honey in diabetic albino rats by collagen assessment. Asian J Nat Prod Biochem 19: 39-44. All humans will experience some type of wound in every lifetime. Most wounds heal quickly with little or no attention, but many people suffer from complex and/or persistent wounds, therefore posing a burden. This study was designed to assess the efficacy of Mitracarpus hirtus (L.) DC. ointment against honey in diabetic rats. To achieve this, percentage wound closure and collagen assessments were used to express treatment efficacy. Results show that on day 21, rats treated with M. hirtus ointment had the highest percentage closure (94.5%), while honey treated and non-treated recorded 90.0% and 83.3%, respectively. Similarly, a significant difference (p < 0.05) was observed on day 21 in the total collagen deposited in wounds of diabetic rats (10.57 ± 0.7) and M. hirtus ointment treated wounds (11.77 ± 0.4) as compared with the non-treated diabetic rats. M. hirtus ointment was efficacious in healing wounds in diabetic rats and healed wounds faster than honey and may hold potential for wound healing in diabetes mellitus sufferers. However, the wound healing mechanism of this ointment needs further investigation.
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References
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[24] Lembong, J, Sabass B, Stone HA. 2017. Calcium oscillations in wounded fibroblast monolayers are spatially regulated through substrate mechanics bioRxiv; 1-16. http://dx.doi.org/10.1101/116426
[25] Cunningham L, Levine G, Bozkurt B, 2018. Heart Failure: Long-term Management. In: Cardiol Sec 5th ed. 241-252. New York: Elsevier
[26] Wang T Gu Q, Zhao J, Mei J, Shao M, Pan Y Zhang J, Wu H, Zhang Z. Liu F. 2015. Calcium alginate enhances wound healing by up-regulating the ratio of collagen types I/III in diabetic rats. Int J Clin Exp Path; 8(6): 6636-6645. PMID: 26261545
[27] Weston AR, Althen TG. 2002. Review: The Role of Collagen in Meat Tenderness. The pro Ani Scit. 18(2): 107-111. https://doi.org/10.15232/S1080-7446(15)31497-2
[28] Ponrasu T. Suguna L. 2014. Efficacy of Annona Squamosa L in the Synthesis of Glycosaminoglycans and Collagen during Wound Repair in Streptozotocin-¬Induced Diabetic Rats. BioMed Res Int? (9) 1–10. http://dx.doi.org/10.1155/2014/124352
[29] Jato JA, Inalegwu B, Onyezili FN. 2021 Antioxidant enzymes in assessment of wound healing efficacy of Mitracarpus villosus ointment in diabetic state. Chron Dis J; 9(1): 7-13 http://dx.doi.org/10.22122/cdj.v9i1.581
[30] Yang J, Chen Z, Ye R, Li J, Lin Y, Gao J, et al. 2018. Touch-actuated microneedle array patch for closedloop transdermal drug delivery. Drug Deliv; 25(1): 1728-39
[31] Ponrasu T, Madhukumar KN, Ganeshkumar M, Iyappan K, Sangeethapriya V, Gayathri VS, Suguna L. 2014. Efficacy of Acorus calamus on collagen maturation on full thickness cutaneous wounds in rats Pharmacogen Mag. 10(2) S299–S305. http://www.phcog.com/text.asp?2014/10/38/299/133283
[32] Kirubanandan S. Swethkamal K, Renganathan S. 2013. Activities of Triphala towards Promoting the Synthesis at wound Site and Inhibiting Methicillin-Resistant Staphylococcus aureus and its Enzymes. Int J Pharm Pharmaceut Sci 5(2): 54-62.
[2] Masharani U, German MS. Pancreatic Hormones and Diabetes mellitus. In: Gardner DG, Shoback D. (eds.) Greenspan's basic & clinical endocrinology 9th ed. New York: McGraw- Hill Medical; 2011. p. 605 Chapter 17.
[3] IDF Diabetes Atlas. Fifth Edition 2011.
[4] Yamany AA, Sayed HM. 2012. Effect of low level laser therapy on neurovascular function of diabetic peripheral neuropathy J. Adv Res. 3: 21–28. http://dx.doi.org/10.1016/j.jare.2011.02.009.
[5] Shankar M, Ramesh B, Roopa KD, Niranjan BM. 2014. Wound Healing and it’s Importance- A Review. Der Pharmacologia Sinica. 1(1): 24-30.
[6] Guo S, DiPietro LA. 2010. Factors Affecting Wound Healing. J Dent Res 89 (3):219-229. Doi: https://doi.org/10.1177/0022034509359125
[7] Damir A. 2011.Why Diabetic Foot Ulcers do not heal? JIMSA 24(4):205-206
[8] Kurahashi T, Fujii J. 2015. Roles of Antioxidative Enzymes in Wound Healing J Dev Biol. 3: 57-70. https://doi.org/10.3390/jdb3020057
[9] Wong VW, Geoffrey C, Gurtner GC, Longaker MT. 2013. Wound Healing: A Paradigm for Regeneration. Mayo Foundation for Medical Education and Research. Mayo Clin Proc. 88(9): 1022-1031. http://dx.doi.org/10.1016/j.mayocp.2013.04.012
[10] Olczyk P, Mencner A, Komosinska-Vassev K. 2014. The Role of the Extracellular Matrix Components in Cutaneous Wound Healing BioMed Res Int, 1-8. http://dx.doi.org/10.1155/2014/747584
[11] Zaja-Milatovic S, Richmond A. 2008. CXC chemokines and their receptors: A case for a significant biological role in cutaneous wound healing Histol. Histopathol. 23(11): 1399-1407. DOI: 10.14670/HH-23.1399
[12] Kim YS, Cho I, Jeong M, Jeong S, Nah SY, Cho Y, Kim SH, Go A, Kim SE, Kang SS, et al, 2011. Therapeutic E?ect of Total Ginseng Saponin on Skin Wound Healing J. Gin Res 35(3): 360-367. http://dx.doi.org/10.5142/jgr.2011.35.3.360.
[13] Upadhyay A, Chattopadhyay P, Goyary D, Mazumder PM, Veer V. 2014. Ixora coccinea Enhances Cutaneous Wound Healing by Upregulating the Expression of Collagen and Basic Fibroblast Growth Factor. Pharmacology; 1-9. http://dx.doi.org/10.1155/2014/751824.
[14] Jato JA, Bawa I, Onyezili FN. 2018b. Phytochemical Analysis of Mitracarpus villosus, and Comparative Toxicity of Mitracarpus villosus Ointment and Honey. Int J Mod Sci and Tech. 3(11): 220-227
[15] Institute for Laboratory Animal Research. Guide for care and use of Laboratory Animal. Washington (DC): National Academies Press [PubMed]. 1996
[16] Jato JA, Bawa I, Onyezili FN. 2018a. Diabetes Induction with Streptozotocin and Insulin Action on Blood Glucose Levels in Albino Rats. Int J Mod Sci and Tech. 3(10): 208-212.
[17] Mendes JJ, Leandro CI, Bonaparte DP, Pinto AL. 2012. A Rat Model of Diabetic Wound Infection for the Evaluation of Topical Antimicrobial Therapies Amr Assoc Lab Ani Sci, 62(1): 37–48. PMID: 22330650
[18] Wong VW, Sorkin M, Glotzbach JP, Longaker MT, Gurtner GC. 2011. Surgical approaches to create murine models of human wound healing. J Biomed Biotechnol.: 969618: 1-8. http://dx.doi.org/10.1155/2011/969618.
[19] Desallais L, Avouac J, Fréchet M, Elhai, M, Ratsimandresy R, Montes M, Mouhsine H, Do H, Zagury J, Allanore Y. 2014. Targeting IL-6 by both passive or active immunization strategies prevents bleomycin-induced skin fibrosis Arth Res & Ther, 16:R157. http://arthritis-research.com/content/16/4/R157
[20] Dwivedi VK, Chaudhary M. 2012.Comparative wound healing efficacy of ampucare and becaplermin in diabetic rat. Afr J Pharm Pharmacol. 6(1): 883-892. http://www.academicjournals.org/AJPP
[21] Barua, CC, Begum SS, Pathak DC, Bora RS. 2013 Wound healing activity of Alternanthera brasiliana Kuntze and its antioxidant profiles in experimentally induced diabetic rats J App Pharm Sci 3(10): 161-166, http://www.japsonline.com. DOI: http://dx.doi.org/10.7324/JAPS.2013.31029.
[22] Sazegar A, Reza A, Hosseini A, Behravan E. 2011. The Effects of Supplemental Zinc and Honey on Wound Healing in Rats. Ira J Bas Med Sci; 14(4): 391-398. PMID: 23493488
[23] Nisbet HO, Nisbet C, Yarim M, Guler, A, Ahmet O. 2010. Effects of Three Types of Honey on Cutaneous Wound Healing WOUNDS; 22(11): 275–283
[24] Lembong, J, Sabass B, Stone HA. 2017. Calcium oscillations in wounded fibroblast monolayers are spatially regulated through substrate mechanics bioRxiv; 1-16. http://dx.doi.org/10.1101/116426
[25] Cunningham L, Levine G, Bozkurt B, 2018. Heart Failure: Long-term Management. In: Cardiol Sec 5th ed. 241-252. New York: Elsevier
[26] Wang T Gu Q, Zhao J, Mei J, Shao M, Pan Y Zhang J, Wu H, Zhang Z. Liu F. 2015. Calcium alginate enhances wound healing by up-regulating the ratio of collagen types I/III in diabetic rats. Int J Clin Exp Path; 8(6): 6636-6645. PMID: 26261545
[27] Weston AR, Althen TG. 2002. Review: The Role of Collagen in Meat Tenderness. The pro Ani Scit. 18(2): 107-111. https://doi.org/10.15232/S1080-7446(15)31497-2
[28] Ponrasu T. Suguna L. 2014. Efficacy of Annona Squamosa L in the Synthesis of Glycosaminoglycans and Collagen during Wound Repair in Streptozotocin-¬Induced Diabetic Rats. BioMed Res Int? (9) 1–10. http://dx.doi.org/10.1155/2014/124352
[29] Jato JA, Inalegwu B, Onyezili FN. 2021 Antioxidant enzymes in assessment of wound healing efficacy of Mitracarpus villosus ointment in diabetic state. Chron Dis J; 9(1): 7-13 http://dx.doi.org/10.22122/cdj.v9i1.581
[30] Yang J, Chen Z, Ye R, Li J, Lin Y, Gao J, et al. 2018. Touch-actuated microneedle array patch for closedloop transdermal drug delivery. Drug Deliv; 25(1): 1728-39
[31] Ponrasu T, Madhukumar KN, Ganeshkumar M, Iyappan K, Sangeethapriya V, Gayathri VS, Suguna L. 2014. Efficacy of Acorus calamus on collagen maturation on full thickness cutaneous wounds in rats Pharmacogen Mag. 10(2) S299–S305. http://www.phcog.com/text.asp?2014/10/38/299/133283
[32] Kirubanandan S. Swethkamal K, Renganathan S. 2013. Activities of Triphala towards Promoting the Synthesis at wound Site and Inhibiting Methicillin-Resistant Staphylococcus aureus and its Enzymes. Int J Pharm Pharmaceut Sci 5(2): 54-62.