The effect of combination of indigenous phosphate solubilizing bacteria of Riau, Indonesia on the available phosphorus and phosphorus uptake of soybean
##plugins.themes.bootstrap3.article.main##
Abstract
Alfiah L, Zul D, Nelvia N. 2018. The effect of combination of indigenous phosphate solubilizing bacteria of Riau, Indonesia on the available phosphorus and phosphorus uptake of soybean. Nusantara Bioscience 10: 146-150. Despite the abundant amount of phosphorus (P) in the soil, P uptake by the plants is very limited. In acidic soil, phosphorus (P) is bound to aluminum (Al) and iron (Fe), whereas in the alkaline soil, phosphorus (P) is bound to calcium (Ca). The improvement of efficiency and availability of P to plants can be made by utilizing a group of solubilizing phosphate microorganisms. Potency test to investigate the P solubility by Phosphate Solubilizing Bacteria (PSB) has been conducted by isolating the bacteria from peat soil at Biosphere Reserves of Giam Siak Kecil Bukit Batu, Riau, Indonesia. The semi-quantitative test revealed that the PSB were able to dissolve Ca3 (PO4)2, FePo4 and phosphate rock. However, the adaptation ability and potency of PSB from indigenous Riau peat soil inoculated into soybean (Glycine max L. Merr) plants in the mineral soil have not yet been investigated. The present study was carried out from March to June 2015 on the alluvial soil in Babussalam Village, Rambah Sub-district, Rokan Hulu District, Riau. The aim of this study was to determine the effect of PSB inoculation on bacterial population and phosphatase activity. The study also aimed to determine the available P and P uptake and their impact on soybean growth and production. The study employed a factorial experiment laid out in a completely randomized design (CRD) consisted of two factors, i.e., soil treatment and PSB. The first factor comprised of two levels, i.e., T0: non-sterilized soil, T1: sterilized soil. The second factor consisted of 4 levels, i.e., B0: without PSB inoculation, B1: inoculation using 2 isolates of PSB (BB_UB6 and BB_K9), B2: inoculation using 3 isolates of PSB (BB_UB6, BB_K9 and BB_K2), and B3: inoculation using 4 isolates of PSB (BB_UB6, BB_K9, BB_K2, and BB_HS13). The results showed that inoculation of starter 3 had the highest phosphatase activity rate of 12.43 ?g p NP g-1 hour-1. The highest available P was produced by starter 2, while the P uptake on non-sterilized soil was higher than that on the sterilized soil at 2.63 mg plant-1. PSB inoculation and soil sterilization did not significantly affect the population of phosphate solubilizing bacteria.
2019-01-01
##plugins.themes.bootstrap3.article.details##
status, microbiota population, potential of mycorrhizal infection and
plant growth. Soil Science 6 (1): 43-52
De Angelis KM. 2006. Microbial Community Ecology and Bacterial
Quorum Sensing as Control Points in Rhizosphere Nitrogen Cycling.
[Dissertation]. The University of California. Berkeley, CA.
Fitriatin BN, Joy B, Subroto T. 2009. The Influence of Organic
Phosphorous Substrate on Phosphatase Activity of Soil Microbes,
Proceeding International Seminar of Chemistry, Universitas
Padjadjaran, Jatinangor, Indonesia, 30-31 Oktober 2008.
Hallmann J, Quadt-Hallmann A, Mahaffee WF, Kloepper JW. 1997.
Bacterial endophytes in agricultural crops. J Microbiol 43(10): 895-
914.
Jones JB, Wolf B, Mills HA. 1991. Plant Analysis Handbook.MicroMacro Publishing, Inc. Georgia. USA.
Kilara A, Harwalkar VR. 1996. Denaturation. In Food Proteins: Properties
and Characterization. VCH Publishers. New York.
Lestari, Wahyu TM, Linda, Martina A. 2011. The ability of seigaro's
phosphate solubilizing bacteria in provision of dissolved phosphate
and its absorption on soybean plants. Biospecies 4 (2): 1-5.
Lines-Kelly R. 2005. Defend The Rhizosphere and Root Against
Pathogenic Microorganism. Department of Primary Industries, State
of New South Wales, Sydney.
Miller MB, Bassler BL. 2001. Quorum sensing in bacteria. Ann Rev
Microbiol 55: 99-165.
Nelson DL, Cox MM. 2000. Lehninger Principles of Biochemistry. 3rd
ed. Worth Publisher, New York.
Rachmiati Y. 1995. Phosphate solubilizing bacteria from plant rhizosphere
and its ability in dissolving phosphate. Proceedings of the National
Congress VI HITI Volume 1. Land Use as a Spatial Planning Tool to
Improve Human's Welfare, Soil Science Association of Indonesia.
Jakarta, 12-15 December 1995.
Saidi D. 2002. Isolation and characterization of cellulolytic bacteria and
phosphate solubilizer from andisol as biological fertilizer agent.
Habitat 8 (4): 201 - 211.
Santosa E. 2009. The activity of Some Bacteria Solubilizing Phosphate
Isolatesat Various Levels C-Organic in Ultisols Soil. Soil Research
Institute. Bogor.
Suliasih R. 2007. Phosphatase activity and calcium phosphate dissolution
by some Phosphate Solubilizing Bacteria. Biodiversitas 8: 23-26.
Syafi S. 2008. Morphological and Physiological Response of Different
Genotypes Seeds of Jatropha (Jatropha curcas L.) to Drought Stress.
[Thesis]. Bogor Agricultural University, Bogor. [Indonesia]
Tamad, Ma’as A, Radjagukguk B, Hanudin E, Widada J. 2013.
Availability of phosphorus in andisol soil for maize (Zea mays L.) by
phosphate solubilizing bacterial inoculum. J Agron. 41 (2): 112-117
Widawati S, Suliasih. 2006. Augmentation of potential Phosphate
Solubilizing Bacteria (PSB) for growth of spur caysim (Brasicaca
ventis Oed.) in marginal land. Biodiversitas 7 (1): 10-14.
Widyati E. 2007. Utilization of sulfate-reducing bacteria for
bioremediation of coal mine former land. Biodiversitas 8 (4): 283-
286.