Effect of IAA and GA3 toward the growing and saponin content of purwaceng (Pimpinella alpina)

Abstract

Abstract. Fathonah D, Sugiyarto. 2009. Effect of IAA and GA₃ toward the growing and saponin content of purwaceng (Pimpinella alpina). Nusantara Bioscience 1: 17-22. The aims of this research are to examine (i) the effect of IAA and GA₃ in different concentrations on the growth of the plants and (ii) the saponin contained inside the P. alpina leaves. The research was done in Sikunang Village, Kejajar Subdistrict, Wonosobo District, Central Java, from July to November 2007. The Completely Random Design used the experimental methods with two factors to analyze this experiment. The first treatment gives IAA and GA₃; the second provides different IAA and GA₃ concentrations. These experiments were repeated three times. Variables measured in this research were plant growth consisting of the number of leaves, their height, width, wet weight, and dry weight. The chemical compound of the secondary metabolite in the form of leave saponin was employed. Analysis of Variance (ANOVA) analyzed the result, then continued to Duncan Multiple Range Test in 5% level to analyze the real difference between those treatments. The result showed that giving IAA and GA₃ differently affects the growth of P. alpina. In variable of the height, the optimal wet weight and dry weight in GA₃ treatment was 50 ppm; an optimum number of leaves in GA₃ treatment was 50 ppm, whereas the leaves width in IAA treatment was 200 ppm and GA₃ treatment was 75 ppm. Optimum saponin treatment was IAA 200 ppm and GA₃ 25 ppm.