Antibacterial activity test of Lactobacillus plantarum isolated from passion fruits (Passiflora edulis) and its relation to plantarisin gene (plnA)

Abstract

Yulinery T, Nurhidayat N. 2015. Antibacterial activity test of Lactobacillus plantarum isolated from passion fruits (Passiflora edulis) and its relation to plantarisin gene (plnA).Pros Sem Nas Masy Biodiv Indon 1 (2): 270-277. Lactobacillus plantarum is able to inhibit the growth of pathogenic bacteria because it produces bacteriocin, known as plantaricin. This study is aimed to test the antibacterial activity and detect the plnA gene of L. plantarum isolated from passion fruits (Passiflora edulis Sims). Ten isolates of L. plantarum were tested for the level of inhibitory activity to bacterial pathogen Escherichia coli and Bacillus cereus using diffusion method. DNA was extracted from selected isolates and the plnA gene was PCR-amplified with its specific primers for plnA. Analysis of plnA gene was done by comparing the level of homology in the Genebank’s data sources. The results showed that L. plantarum isolates MarB4, Mar8, MarA7 and MarA5 exhibited larger inhibition zone than the other isolates. The PCR amplification showed a positive indication of gene plnA with product size about 400-500 bp. Sequencing analysis showed 100% homology with L. plantarum isolates, suggesting that the gene can be identified as plnA gene responsible for the antimicrobial activity.