Stability test of probiotic Lactobacillus plantarum Mar8 encapsulated in oralit using viability analyses

Abstract

Triana E, Yulinery T. 2015. Stability test of probiotic Lactobacillus plantarum Mar8 encapsulated in oralit using viability analyses. Pros Sem Nas Masy Biodiv Indon 1 (2): 278-282. Diarrhea should be quickly and appropriately treated. Otherwise, excessive loss of fluids and minerals from the body can cause dehydration. Dehydration is a very critical condition, because it may lead to hypovolemic shock, even death. When dehydrated, the loss of fluids and electrolytes should be replaced immediately by oral rehydration solution (ORS) that contains electrolytes, such as oralit. In addition, probiotics consumption is very important because probiotics could recover the balance of ‘good’ microbes and ‘bad’ microbes in the digestive system that is once disturbed by diarrhea. However, nowadays, oralit products which contain probiotics in one package are commercially unavailable. Both supplements are usually available separately. Therefore, this research was aimed to develop an oralit product which contains probiotic Lactobacillus plantarum Mar8 as its added value. Stability test of this probiotics in various temperature was done using viability analyses. The results showed that L. plantarum Mar8 in oralit could meet the standard of minimum probiotic amounts, in various storage temperature after various time periods. Its viability was 4.8 x 106 CFU/g in 4oC, 1.25 x 106 CFU/g in 25o C, 8.5 x 106 CFU/g in 37o C, and 2.5 x 107 CFU/g in 42o C. Lactobacillus plantarum Mar8 survived in various time duration and in various storage temperature. It may last for up to 17.77 days, 14.42 days, 9.85 days, and 12.14 days in 4o C, 25oC, 37oC and 42o C, respectively. Based on these data, the most stable of probiotic L. plantarum Mar8 in oralit would be in 4o C-storage, lasting for 17.77 days.